[Histonet] RE: Prolonged FFPE slide and block storage

[Helen Fedor]

Hello, We have done a study and found that storage in small zip-loc bags at -20 is an inexpensive and convenient way to store slides. In fact the slides stored for 5 years at -20 stain better than freshly cut sections from the same blocks. This was also published recently by others. Dr Rimm at Yale does have the storage under nitrogen gas system that is available commercially.


Helen L. Fedor 

Tissue Microarray Lab, Manager
Prostate Spore Lab, Manager
Johns Hopkins University
600 N. Wolfe St, | Marburg Room 406
Baltimore, MD | 21287-7065

410.614.1660




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Saturday, May 15, 2010 9:36 AM
To: SamuelPerry; histonet <@t> lists.utsouthwestern.edu; TimMorken
Subject: Re: [Histonet] RE: Prolonged FFPE slide and block storage

Inexpensive long term anti oxidizing storage? Immerse them in mineral oil!
René J.

--- On Fri, 5/14/10, Morken, Tim <Timothy.Morken <@t> ucsfmedctr.org> wrote:


From: Morken, Tim <Timothy.Morken <@t> ucsfmedctr.org>
Subject: [Histonet] RE: Prolonged FFPE slide and block storage
To: "Perry, Samuel" <Samuel_Perry <@t> DFCI.HARVARD.EDU>, "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Friday, May 14, 2010, 5:05 PM


I have a paper from Yale several years ago about their tissue array facility. They found that storage in nitrogen helped a lot. I will try to find it, but you may be able to google it faster. 

Tim Morken
Supervisor, Histology / IPOX
UCSF Medical Center
San Francisco, CA  


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Perry, Samuel
Sent: Friday, May 14, 2010 1:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Prolonged FFPE slide and block storage

Hi All,
We have a growing need for storing slides. 

They need to be stored in a way that they won't become oxidized and loose their
antigenicity, since we use them for IHC.  

Any suggestions for inexpensive methods for prolonged storage of FFPE slides and
blocks is greatly appreciated.

Thanks!

Sam Perry

Research Technician 
Dana-Farber Cancer Institute
Boston, MA


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