[Histonet] CYP.07680 - cytology cross contamination

[DKBoyd <@t> chs.net]

Brandi,
Body effusions have a high potential for cross contamination during the 
staining procedure.  Body fluids ie:  pleural fluid, peritoneal fluid, 
etc.  For that reason they are not stained with other non-gyn specimens 
ie:  bronch. wash, esoph. brush, CSF etc.  Also after the staining all 
solutions up to the 95% before the OG-6 are dumped and refilled.
The Hematoxylin, OG-6 and EA65 are filtered.  The 95% after the EA-65 is 
dumped and alcohols are rotated up.
This should prevent cross contamination.  Written documentation should be 
your procedure and proof that you are changing/filtering solutions.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical 
Center I 
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F: 
804-765-5582 l dkboyd <@t> chs.net







Brandi Higgins <brandihiggins <@t> gmail.com> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
05/11/2010 03:28 PM

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Subject
[Histonet] CYP.07680 - cytology cross contamination






Hello all,

For CAP policy CYP.07680 for "procedures to prevent cross-contamination of
specimens during processing and staining" - what are your labs doing?

 I work in a small lab so the histotechs process the cytology specimens 
and
the pathologists read the slides (we have no PA's or cytoprep techs or
cytotechs to screen slides).  We also process only non-GYN, so we don't 
have
to worry about GYN/non-GYN cross contamination.

The notes under this policy say procedures must prevent 
cross-contamination
between highly cellular specimens and suggest the screening method of
toluidine blue stain to determing if specimens are highly cellular.

Does anyone use the toluidine blue for this purpose?  If so could you tell
me the procedure for toluidine blue you use?  And how do you determing 
which
specimens you stain with toluidine blue and what qualifies as highly
cellular.  If so do you retain these toluidine blue slides for any period 
of
time?

CAP policy also suggests inserting a clean blank slide in each stain run 
and
examine for contamination.  Is anyone doing this?

We have been inspected before with no problems with this CAP question, but 
I
just want to make sure we are doing everything we can to prevent
cross-contamination.

Thanks in advance for your input!
Brandi Higgins, HT (ASCP)
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