[Histonet] Forwarding Request for Control Mehtod

[Tony Henwood]

Ian,

What I have done in the past is to raid the Staff Fridge (or your son's
bedroom!).
There is usually old food left there that has gone moldy - bread or even
strawberries.
Wearing gloves & in a fume hood slice the bread or fruit into slices
(ensuring visible growth of fungi is present), fix for two or more days
in NBF, process as usual.

Voila - fungi controls

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead 
Cnr Hawkesbury Road and Hainsworth Street, Westmead 
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of WILLIAM
DESALVO
Sent: Wednesday, 12 May 2010 4:01 AM
To: histonet
Subject: [Histonet] Forwarding Request for Control Mehtod



> I wonder if you could help me find someone who would kindly donate a 
> paraffin tissue block containing candida or other fungi to be used as 
> a control block. We have had difficulty in obtaining such tissue and 
> have tried all the hospitals in northern Ireland for a tissue block 
> suitable enough to act as a control. Many of the pathology departments

> are in the same position as ourselves in that the control tissue being

> used is getting in short supply.
> 
> I tried to make one using a culture from microbiology with fresh lambs

> lung from a local abattoir but unfortunately the tissue seems to stain

> with PAS as well and the candida where obscured although visible 
> probably due to post mortem changes in the tissue.
 
> Alternatively have any of your members successfully produced fungal 
> control material by other means.
> 
> Thanking you in advance
 
> Ian Clarke
> Biomedical Scientist
> Cellular Pathology Department
> Craigavon Area Hospital
> Sothern Health and Social Services Trust.
> 66 Lurgan Road
> Craigavon
> BT63 5QQ
> Northern Ireland


William DeSalvo, B.S., HTL(ASCP)



 		 	   		  
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