[Histonet] Special stain to demonstrate Silver
DeGuzman, Maria
mdeguzman <@t> lifecell.com
Fri May 7 07:00:25 CDT 2010
Hello Histoneters,
Is there a special stain to detect silver in tissue? If there is please share the procedure.
Thanks,
Fanny
Maria V. De Guzman| Histology Technician I
Main 908.947.1100 Fax 908.947.1085
Direct 908.947.1482 Email mdeguzman <@t> lifecell.com
Mobile 732.688.1386 www.Lifecell.com
LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Thursday, May 06, 2010 2:16 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 78, Issue 7
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Today's Topics:
1. clo test (Tench, Bill)
2. IDEA FOR A NEW RECYCLER!!!! (Madary, Joseph)
3. RE: RE: Barcode and Tracking Information (Rae Staskiewicz)
4. CD 133 (Ingles Claire )
5. Disposable blade holder - amateur microscopist looking for a
cheap one! (Gordon Brown)
6. Disposable blade holder - amateur microscopist looking for a
cheap one! (Gordon Brown)
7. NSH Region II Meeting-discount hotel rate deadline is coming
up! (Goodwin, Diana)
8. dehydration of hydrated slide.... (Eva Permaul)
9. RE: dehydration of hydrated slide.... (Sebree Linda A)
10. RE: dehydration of hydrated slide.... (Mauger, Joanne)
11. B5 fixative (histotech <@t> imagesbyhopper.com)
12. myocyte damage (Bartlett, Jeanine (CDC/OID/NCZVED))
13. RE: Responses to IHC CAP Validation question
(tonia.richmond <@t> gracepathology.com)
14. Starting up new lab (Shaw, Sharon)
15. Formalin fixation time for breast specimens (Richard Cartun)
16. RE: Responses to IHC CAP Validation question
(BSullivan <@t> shorememorial.org)
17. Re: myocyte damage (Merced M Leiker)
----------------------------------------------------------------------
Message: 1
Date: Wed, 5 May 2010 13:57:20 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] clo test
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863055 <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii
The Clo test is a clinical lab test. You need to go to that part of the
CPT coding book (sorry I don't have it available). 88300 is an anatomic
code (gross only, ie, it requires examination of a piece of tissue or
foreign body) and is entirely inappropriate for this test.
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histonet-request <@t> lists.utsouthwestern.edu
Sent: Wednesday, May 05, 2010 1:36 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [BULK] Histonet Digest, Vol 78, Issue 6
Hi everyone,
I am looking to see what CPT code everyone is using for reading Clo
Tests in the pathology department. I have heard of using 87081 but I am
not sure if this is accurate as this is for culture and the CLO is
biochemical reaction not a culture. Currently I have been using 88300
gross only.
Any help would be appreciated.
Thank you,
Amy Farnan
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Message: 2
Date: Wed, 5 May 2010 17:22:22 -0400
From: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>
Subject: [Histonet] IDEA FOR A NEW RECYCLER!!!!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29A3CB81288E6F4BA2C9B3C8015A9A1301A37FFA <@t> MD1EV002.medimmune.com>
Content-Type: text/plain; charset="us-ascii"
Some manufacturer should find a way where you can add all of the
chemicals together in one container and when the recycler runs it spits
out formalin in one, xylene/hemo-de/f83/ in another and alcohol in
another all from one run.
To opine on another histnet query from earlier
I think both have their place. I use CBG for xylene, form, hemo de and
alcohol using the hemo on the processor and depar, alcohol on processor
for low grade alc, and xyle for all but the last xylen on the stainer
using frsh for that. Creative waste is good and simple but the only
thing is mixing old and new formalin can't be good long term. What I
was thinking about doing was using creative waste gravimetric for the
first run, and then after that use the CBG to redistill NBF for round 2,
3, 4 etc. Hey where am I getting all this money and space? Still like
BR recycler too, just do not have one anymore, but liked it. All
recycling is good as long as people know which ones to use. Still seems
to be an issue for some people. I worked in a place where the techs
thought you could throw everyting in one container and the rcycler would
spit out clean alcohol, xylene and formalin from one collective run.
Hey manufactures?
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.
------------------------------
Message: 3
Date: Wed, 5 May 2010 17:32:47 -0500
From: "Rae Staskiewicz" <raestask <@t> grics.net>
Subject: RE: [Histonet] RE: Barcode and Tracking Information
To: "'Mahoney,Janice A'" <Janice.Mahoney <@t> alegent.org>, "'Maggie
Allen'" <maggie.allen <@t> nicewareintl.com>,
<histonet <@t> lists.utsouthwestern.edu>
Cc: histonet-request <@t> lists.utsouthwestern.edu,
":histonet-bounces"@lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID: <926351C712074D27B3AC517297DF0A72 <@t> your4105e587b6>
Content-Type: text/plain; charset="us-ascii"
Jan,
Ditto! Ditto! Ditto! Couldn't have said it better myself!
Rae Staskiewicz
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Mahoney,Janice A
Sent: Wednesday, May 05, 2010 2:53 PM
To: 'Maggie Allen'; histonet <@t> lists.utsouthwestern.edu
Cc: histonet-request <@t> lists.utsouthwestern.edu;
":histonet-bounces"@lists.utsouthwestern.edu;
histonet-bounces <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Barcode and Tracking Information
Blake,
I think I put in my comments about Ventana's Vantage when you first posted
the question, but here goes again.. It is wonderful. Vantage is the best
thing to come along in my lifetime as a histo tech! Wonderful for patient
safety, easy for the techs to use and a manager's dream for all the data it
can provide.
Jan Mahoney
Omaha, NE
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Maggie Allen
Sent: Wednesday, May 05, 2010 2:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Cc: histonet-bounces <@t> lists.utsouthwestern.edu;
":histonet-bounces"@lists.utsouthwestern.edu;
histonet-request <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Barcode and Tracking Information
Hello Blake,
LabelClinic HTS is a tracking system for Histology artifacts within the
Pathology workflow. The product is designed to provide improved quality
control and traceability with the lab. It provides flexible and reliable
labeling and tracking of histology requisitions, containers, specimens,
blocks and slides. Using bar code technology, LabelClinic HTS provides an
optimized workflow that reduces errors and significantly enhances patient
safety. It can be used stand alone, or integrated into an LIS / AP software
system.
Benefits:
* Flexible lab configuration for custom lab workflow
* Reduce errors and increase efficiency
* Just-in-time reporting of artifact locations or last know location
* Alerting for sub-process time violations and missing artifacts
* Deploy to Desktop PC's or mobile hand held's
* Configurable workflow
* Supports color selection for slide and cassette printers
I would be happy to set up an online WebEx demo of the system for anyone who
may be interested. Thank you!
Maggie Allen
Healthcare Business Development Manager
Niceware International, LLC
200 South Executive Drive
Suite 200
Brookfield, Wisconsin 53005
Tel (810) 629-3930
Cell (215) 200-0268
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------------------------------
Message: 4
Date: Wed, 5 May 2010 22:15:03 -0500
From: "Ingles Claire " <CIngles <@t> uwhealth.org>
Subject: [Histonet] CD 133
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<F2F030053F9B7345831BED293A6D57E103A1A5B0 <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="iso-8859-1"
Thought I'd try again...
Anyone know where I can send some slides for a CD133?
Claire
------------------------------
Message: 5
Date: Thu, 6 May 2010 09:16:36 +0100
From: "Gordon Brown" <gordon <@t> 10db.co.uk>
Subject: [Histonet] Disposable blade holder - amateur microscopist
looking for a cheap one!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <PIEPJOGJHCPIEPJKIHPCEEOKEBAA.gordon <@t> 10db.co.uk>
Content-Type: text/plain; charset="iso-8859-1"
I dabble in microscopy - well I say I dabble, my wife says otherwise! - and
I have a reasonably well equipped home lab, which includes the ubiquitous
Cambridge Rocker. I've had reasonably good results with plant tissue
sections but sharpening the blades has always proved to be a pain and I tend
to get mixed results. However, I've recently acquired a bunch of Accu Edge
low profile blades at very low cost but I'm unable to source a holder at an
affordable price. IS there anyone out there in professional histology land
who has for sale a used, even rough condition holder suitable for these
blades? You'd get the grateful thanks of both myself and my wife, who will
be more than pleased to see me spend less time muttering about my poor
sharpening skills!
Many thanks
Gordon (UK)
------------------------------
Message: 6
Date: Thu, 6 May 2010 09:36:41 +0100
From: "Gordon Brown" <gordon <@t> 10db.co.uk>
Subject: [Histonet] Disposable blade holder - amateur microscopist
looking for a cheap one!
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <PIEPJOGJHCPIEPJKIHPCIEOKEBAA.gordon <@t> 10db.co.uk>
Content-Type: text/plain; charset="iso-8859-1"
Perhaps I should point out that despite the date in the UK, my name is
genuinely as given, although my namesake may well be looking for a new hobby
to fill in his spare time after today...........
Gordon
------------------------------
Message: 7
Date: Thu, 6 May 2010 08:18:01 -0400
From: "Goodwin, Diana" <Goodwd2 <@t> LabCorp.com>
Subject: [Histonet] NSH Region II Meeting-discount hotel rate deadline
is coming up!
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EB4103C86804F442B0D76D16CBB09363D73B7D32AA <@t> RTWECS03.lca.labcorp.com>
Content-Type: text/plain; charset="windows-1252"
You don?t want to miss the upcoming Region II Meeting on June 10-12 in Atlantic City NJ! 25 speakers are presenting 30 different topics from wet workshops to short seminars. CEUs will be granted for all sessions. Over 30 vendors are participating in our Exhibit Area. Your registration fee includes free admission to the Vendor Exhibit, AM and PM Breaks, buffet lunch and Friday evening?s reception. The hotel room rate is reduced to $89/night, but you must reserve by Monday, May 10th! The mail-in registration deadline is May 20th. For more information, you can download a meeting brochure from the NSH website under state meetings at www.nsh.org/content/region-ii-meeting<http://www.nsh.org/content/region-ii-meeting>.
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Message: 8
Date: Thu, 06 May 2010 08:56:01 -0400
From: Eva Permaul <eca9 <@t> georgetown.edu>
Subject: [Histonet] dehydration of hydrated slide....
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4BE2BC61.2040701 <@t> georgetown.edu>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed
Good morning,
I accidentally hydrated a FFPE slide that I can not stain today. It is
in water. I did not do antigen retrieval. What do I do? Can I dehydrate
the slide again? Will I be able to stain it later if I do?
Thanks,
Eva Permaul
Georgetown University
------------------------------
Message: 9
Date: Thu, 6 May 2010 08:49:06 -0500
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] dehydration of hydrated slide....
To: "Eva Permaul" <eca9 <@t> georgetown.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8C023B4AB999614BA4791BAEB26E2738399E3B <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="US-ASCII"
Eva,
I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to
stain. You might also refrigerate the slide in buffer if its going to
be overnight.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva
Permaul
Sent: Thursday, May 06, 2010 7:56 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] dehydration of hydrated slide....
Good morning,
I accidentally hydrated a FFPE slide that I can not stain today. It is
in water. I did not do antigen retrieval. What do I do? Can I dehydrate
the slide again? Will I be able to stain it later if I do?
Thanks,
Eva Permaul
Georgetown University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 10
Date: Thu, 6 May 2010 11:02:59 -0400
From: "Mauger, Joanne" <MAUGER <@t> email.chop.edu>
Subject: RE: [Histonet] dehydration of hydrated slide....
To: Sebree Linda A <LSebree <@t> uwhealth.org>, Eva Permaul
<eca9 <@t> georgetown.edu>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<443F5B475A9BF647AB962E834884EBAD2788770DE6 <@t> EX7CCRPW03V1.chop.edu>
Content-Type: text/plain; charset="us-ascii"
Eva,
If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide.
Joanne Mauger HT(ASCP)QIHC
Children's Hospital of Philadelphia
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [LSebree <@t> uwhealth.org]
Sent: Thursday, May 06, 2010 9:49 AM
To: Eva Permaul; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] dehydration of hydrated slide....
Eva,
I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to
stain. You might also refrigerate the slide in buffer if its going to
be overnight.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva
Permaul
Sent: Thursday, May 06, 2010 7:56 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] dehydration of hydrated slide....
Good morning,
I accidentally hydrated a FFPE slide that I can not stain today. It is
in water. I did not do antigen retrieval. What do I do? Can I dehydrate
the slide again? Will I be able to stain it later if I do?
Thanks,
Eva Permaul
Georgetown University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 11
Date: Thu, 6 May 2010 11:20:01 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: [Histonet] B5 fixative
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <A00E24ADAFD4471DAC50D9718C35368F <@t> hopperPC>
Content-Type: text/plain; charset="US-ASCII"
I was under the impression that B5, because of the mercury content, was
outlawed for use Jan 1, 2005. But now I am not so sure! Can anyone tell me
if there is a federal law regarding this? We no longer use B5, we use B+,
but I know someone in OK who is using B5 (I am in FL). Is he breaking any
laws by using it and/or should he be switched to an alternative like B+?
Thanks!
Michelle
------------------------------
Message: 12
Date: Thu, 6 May 2010 11:16:04 -0400
From: "Bartlett, Jeanine (CDC/OID/NCZVED)" <jqb7 <@t> cdc.gov>
Subject: [Histonet] myocyte damage
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<68510B12184E45498EABD4CB6F3868FE012870BC <@t> LTA3VS001.ees.hhs.gov>
Content-Type: text/plain; charset=us-ascii
Hello everyone,
I am in need of a special stain or an IHC that will demonstrate myocyte
damage in muscle tissue...esp. cardiac.
Any help will be greatly appreciated.
Thanks!
Jeanine Bartlett
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA 30333
(404) 639-3590
jeanine.bartlett <@t> cdc.hhs.gov
------------------------------
Message: 13
Date: Thu, 6 May 2010 10:27:50 -0500
From: tonia.richmond <@t> gracepathology.com
Subject: RE: [Histonet] Responses to IHC CAP Validation question
To: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "thisisann <@t> aol.com"
<thisisann <@t> aol.com>
Message-ID:
<OF2B2D84A8.322A1680-ON8625771B.0054F24C-8625771B.0054F24D <@t> gracepathology.com>
Content-Type: text/plain; charset="UTF-8"
If you are a brand new lab, how do you validate IHC if you are no yet receiving patient specimens? Can the validation be done on cont rol tissues only?
Sincerely,
Tonia Richmond, AS, HT (ASCP)
Chief Operations Officer Grace Pathology
PH: (501) 765-7367
Email:
-----histonet-bounces <@t> lists.utsouthwestern.edu wrote: -----
<
To: "thisisann@ "histonet <@t> lists.utsouthwestern.edu" < ;histonet <@t> lists.utsouthwestern.edu>
From: "McMahon, Loralee A" <Lo Sent by: histonet-bounces <@t> lists.u Date: 04/28/2010 02:01PM
Subject: RE: [Histonet] Re Any inspection that I have under case rule. Except for the Er/Pr//Her-2 cases. We also use a TMA to make our live contains known positives and known negatives.
I cases i markers (SV- in a slide that w then you can say it has spe Any inspector that I have come across is usually understanding this. But I am sure that there are exceptions to this.........esp ecially if they are not familiar with immunohistochemistry.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong M Department of Surgical Pathology
(585) 275-7210
______________________ 5F__ From: histonet-bounces <@t> lis [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf thisisann <@t> aol.com [thisisann <@t> aol.com]
Sent: Wednesday, April 28, 201 To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] R The following is one respone 1. I asked CAP who told me that they do not currentl guideline on
validating but that they
recommend what is in t Quality Management In Anatomic Pathology, Promoting P Safety
Through Systems Improvement and Error
by Raouf E. Nakhl sold by CAP !
Chapter 8- That is what we follow.
I. Get a new antib II. Once optimized you n positive
(how many?)
"a suffien III. Must also be run on cases expected to be negative. (how IV. In a situation where you cannot expect a lot of cases or such a
case has
never been presented in your lab, then you must say just (ex. some of the hormones we just use a pituitary)
___________________ ______________________ 5F__ Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
______________________ 5F__ ______________________
Histo Histonet <@t> lists.utsouthwestern.edu
[3]http://l
References
1. 3D"mailto:tonia.richmond <@t> gracepathology.com"
2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
3. 3D"http://=/
------------------------------
Message: 14
Date: Thu, 6 May 2010 11:42:40 -0400
From: "Shaw, Sharon" <shshaw <@t> WPI.EDU>
Subject: [Histonet] Starting up new lab
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<AA21DA463C7B1143A22FAF799668F05F547ADC781A <@t> EXCHANGEMAIL.admin.wpi.edu>
Content-Type: text/plain; charset="us-ascii"
I'm looking at starting up a new histology lab and need everything, I have a tight budget. Can anybody give me recommendations on where to buy refurbished equipment.
Thanks
Sharon
------------------------------
Message: 15
Date: Thu, 06 May 2010 11:57:43 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Formalin fixation time for breast specimens
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4BE2AEB7.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII
There has been a lot of discussion recently regarding recommendations for formalin fixation of breast specimens. If you are interested in this topic please read the following article published in the May 2010 issue of the American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time does not affect the expression of estrogen receptor".
Richard
Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
(860) 545-1596 Office
(860) 545-2204 Fax
------------------------------
Message: 16
Date: Thu, 6 May 2010 12:16:45 -0400
From: BSullivan <@t> shorememorial.org
Subject: RE: [Histonet] Responses to IHC CAP Validation question
To: tonia.richmond <@t> gracepathology.com
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>, "McMahon, Loralee A"
<Loralee_Mcmahon <@t> URMC.Rochester.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu, "thisisann <@t> aol.com"
<thisisann <@t> aol.com>
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<OF88A1FD4E.77204CC6-ON8525771B.00591B88-8525771B.00599A19 <@t> shorememorial.org>
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One important thing to remember is that you should make sure All material
being used for validation is processed the same way. Will your control
tissue be purchased or processed in your lab? Control tissue is used for
validation but you should use tissue with various levels of positivity.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590
tonia.richmond <@t> gr
acepathology.com
Sent by: To
histonet-bounces@ "McMahon, Loralee A"
lists.utsouthwest <Loralee_Mcmahon <@t> URMC.Rochester.edu
ern.edu >
cc
"histonet <@t> lists.utsouthwestern.edu"
05/06/2010 11:27 <histonet <@t> lists.utsouthwestern.edu>
AM , "thisisann <@t> aol.com"
<thisisann <@t> aol.com>
Subject
RE: [Histonet] Responses to IHC CAP
Validation question
If you are a brand new lab, how do you validate IHC if you are no= t
yet receiving patient specimens? Can the validation be done on cont
rol tissues only?
Sincerely,
Tonia Richmond, AS, HT (ASCP)
Chief Operations Officer = / Laboratory
Grace Pathology
PH: (501) 765-7367
Email: = [1]tonia.= richmond <@t> gracepathology.com
-----histonet-bounces <@t> lists.utsouthwestern.edu wrote: -----
<= /FONT>
To: "thisisann@= aol.com" <thisisann <@t> aol.com>,
"histonet <@t> lists.utsouthwestern.edu" <
;histonet <@t> lists.utsouthwestern.edu>
From: "McMahon, Loralee A" <Lo= ralee_Mcmahon <@t> URMC.Rochester.edu>
Sent by: histonet-bounces <@t> lists.u= tsouthwestern.edu
Date: 04/28/2010 02:01PM
Subject: RE: [Histonet] Re= sponses to IHC CAP Validation question
Any inspection that I have under= gone we have used the 25 to 30
case rule. Except for the Er/Pr//Her-2= . We use closer to 50
cases. We also use a TMA to make our live= s easier. The TMA
contains known positives and known negatives.
I= n cases of t-cell or b-cell markers or cytokeratins. 25 to 30
cases i= s easy. But when you are validated for more hard to find
markers (SV-= 40) then fewer cases is acceptable. We always throw
in a slide that w= e know will not stain for sv-40 like a tonsil -
then you can say it has spe= cificity.
Any inspector that I have come across is usually understanding= of
this. But I am sure that there are exceptions to this.........esp
ecially if they are not familiar with immunohistochemistry.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong M= emorial Hospital
Department of Surgical Pathology
(585) 275-7210
______________________ 5F__= _______________
From: histonet-bounces <@t> lis= ts.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf= Of
thisisann <@t> aol.com [thisisann <@t> aol.com]
Sent: Wednesday, April 28, 201= 0 2:47 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] R= esponses to IHC CAP Validation question
The following is one respone= I rec'd:
1. I asked CAP who told me that they do not currentl= y have a
guideline on
validating but that they
recommend what is in t= he following book:
Quality Management In Anatomic Pathology, Promoting P= atient
Safety
Through Systems Improvement and Error
by Raouf E. Nakhl= eh, MD & Patrick Fitzgibbons, MD editors
sold by CAP !
Chapter 8-= Quality Management in IHC
That is what we follow.
I. Get a new antib= ody and optimize it with your positive control.
II. Once optimized you n= eed to run it on cases expected to be
positive
(how many?)
"a suffien= t size ..."
III. Must also be run on cases expected to be negative. (how= many?
IV. In a situation where you cannot expect a lot of cases or such
a
case has
never been presented in your lab, then you must say just = that.
(ex. some of the hormones we just use a pituitary)
___________________ ______________________ 5F__= ___
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
= [2]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
______________________ 5F__ ______________________
Histo= net mailing list
Histonet <@t> lists.utsouthwestern.edu
[3]http://l= ists.utsouthwestern.edu/mailman/listinfo/histonet
References
1. 3D"mailto:tonia.richmond <@t> gracepathology.com"
2. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"
3. 3D"http://=/
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------------------------------
Message: 17
Date: Thu, 06 May 2010 12:30:50 -0400
From: Merced M Leiker <leiker <@t> buffalo.edu>
Subject: Re: [Histonet] myocyte damage
To: "Bartlett, Jeanine (CDC/OID/NCZVED)" <jqb7 <@t> cdc.gov>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5AFA0A6D83365A1F044D4E16 <@t> CDYwxp1931.ad.med.buffalo.edu>
Content-Type: text/plain; charset=us-ascii; format=flowed
Stain with cTnI and inspect for damage visually. Just a guess.
Regards,
Merced
--On Thursday, May 06, 2010 11:16 AM -0400 "Bartlett, Jeanine
(CDC/OID/NCZVED)" <jqb7 <@t> cdc.gov> wrote:
> Hello everyone,
>
> I am in need of a special stain or an IHC that will demonstrate myocyte
> damage in muscle tissue...esp. cardiac.
>
> Any help will be greatly appreciated.
>
> Thanks!
> Jeanine Bartlett
> Centers for Disease Control and Prevention
> Infectious Diseases Pathology Branch
> 1600 Clifton Road, MS/G-32
> 18/SB-114
> Atlanta, GA 30333
> (404) 639-3590
> jeanine.bartlett <@t> cdc.hhs.gov
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
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