AW: [Histonet] Formalin fixation time for breast specimens

[Gudrun Lang]

I've read only the abstract and some infos regarding this article in the
internet.
This group used a short processing protocol (2:30), didn't they? I think
that hampers the comparability with standard overnight processing. And I
missed the longer fixation tests.
They mentioned, that Goldstein left the tissue in 100% cold ethanol before
processing. I assume, that ethanol-fixation is the cause for weaker staining
in underfixed areas.

How are the commonly known edge-effects in ER-staining with stronger
staining in the outer areas to explain, if not with insufficient fixation?

It could be possible, that the longer incubation with processing reagents
has effects on underfixed areas, that were not obvious in their experiment
because of the short processing. 

Hm, I'm not convinced.
Gudrun



-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Richard
Cartun
Gesendet: Donnerstag, 06. Mai 2010 17:58
An: Histonet
Betreff: [Histonet] Formalin fixation time for breast specimens

There has been a lot of discussion recently regarding recommendations for
formalin fixation of breast specimens.  If you are interested in this topic
please read the following article published in the May 2010 issue of the
American Journal of Clinical Pathology by Ibarra JA, et al., "Fixation time
does not affect the expression of estrogen receptor".

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax



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