[Histonet] dehydration of hydrated slide....

[Mauger, Joanne]

Eva,
If you dehydrate it again to 100% or xylene, it is less likelt to fall off the slide.
Joanne Mauger HT(ASCP)QIHC
Children's Hospital of Philadelphia
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A [LSebree <@t> uwhealth.org]
Sent: Thursday, May 06, 2010 9:49 AM
To: Eva Permaul; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] dehydration of hydrated slide....

Eva,

I would hold it in a buffer, i.e. Tris, PBS, etc. til you're ready to
stain.  You might also refrigerate the slide in buffer if its going to
be overnight.


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva
Permaul
Sent: Thursday, May 06, 2010 7:56 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] dehydration of hydrated slide....

Good morning,
I accidentally hydrated a FFPE slide that I can not stain today. It is
in water. I did not do antigen retrieval. What do I do? Can I dehydrate
the slide again? Will I be able to stain it later if I do?
Thanks,
Eva Permaul
Georgetown University


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