[Histonet] test
DeGuzman, Maria
mdeguzman <@t> lifecell.com
Wed May 5 12:11:21 CDT 2010
test
Maria V. De Guzman| Histology Technician I
Main 908.947.1100 Fax 908.947.1085
Direct 908.947.1482 Email mdeguzman <@t> lifecell.com
Mobile 732.688.1386 www.Lifecell.com
LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, May 04, 2010 1:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 78, Issue 4
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Today's Topics:
1. Region II Symposium (Barone, Carol )
2. Switching Back to Formalin (Jones, Laura)
3. RE: Switching Back to Formalin (Bauer, Karen L.)
4. RE: Switching Back to Formalin (WILLIAM DESALVO)
5. ? about a Leica RM2155 microtome (jstaruk)
6. mouse kidney frozen sectioning (Andrew Burgeson)
7.
C1FE5960057C084CA389CE97779062904EBD096D <@t> TCDMSG01.ad.TexasChildrensHospital.org
(Andrew Burgeson)
8. Re: ? about a Leica RM2155 microtome (Jack Ratliff)
9. Trichrome Help (Drew Meyer)
10. RE: Trichrome Help (Monfils, Paul)
11. Re: mouse kidney frozen sectioning (Michelle MacVeigh-Aloni)
12. Re: ? about a Leica RM2155 microtome (Pat Laurie)
13. specimen disposal (Gervaip <@t> aol.com)
14. Re: ? about a Leica RM2155 microtome
(MariAnn.Mailhiot <@t> leica-microsystems.com)
15. Formalin and Kylen recycling (mbbarra)
16. waterbath residue (path lab)
17. CBG BIOTECH RECYCLER (srishan <@t> mail.holyname.org)
18. grossing qualifications (Donna M. Nolan)
19. RE: waterbath residue (Mahoney,Janice A)
20. RE: CBG BIOTECH RECYCLER (Liz Chlipala)
21. Re: waterbath residue (Lynette Pavelich)
22. Re: waterbath residue (Rene J Buesa)
----------------------------------------------------------------------
Message: 1
Date: Mon, 3 May 2010 13:29:28 -0400
From: "Barone, Carol " <cbarone <@t> NEMOURS.ORG>
Subject: [Histonet] Region II Symposium
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<37E4BAC017F57141AF64FAA5AEB04CE8033A7CA6 <@t> wlmmsx01.nemours.org>
Content-Type: text/plain; charset="us-ascii"
Histonetter's: For anyone planning to attend the Region II Sympoium....I
wanted to give you a quick reminder that Monday May 10th, 2010 is the
final day to get the discounted room rate at the Clarion Hotel for the
Region II.. If you need information on the meeting you can find it
through the NJ Society or any state society in the region ( DE, MD, PA,
NJ, VA, W.VA amd District of Columbia)...and also NSH. The vendors
attending will be many, and this will present a rare opportunity to get
thos needed CEUs and network as well. Check out the program...make your
selections...but, hurry on those discounted room rates....time is
running out for that one!
As the Region Director, I look forward to touching base with you all on
the questions and suggestions you have for our Region! See you soon!
Carol Barone
------------------------------
Message: 2
Date: Mon, 3 May 2010 14:15:45 -0400
From: "Jones, Laura" <lpjones <@t> srhs-pa.org>
Subject: [Histonet] Switching Back to Formalin
To: "Histonet (E-mail)" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4AE8039AEA096143B965CBC6D0921668022CA806BA <@t> EXCH2007.srhs-pa.org>
Content-Type: text/plain; charset="iso-8859-1"
After years of working to get away from the use of Formalin in our lab, it seems as though we are going to be bringing it back. I'm seeking advice concerning what everyone is using to neutralize their formalin prior to disposal. Any recommendations welcome! Thanks in advance! Laura
------------------------------
Message: 3
Date: Mon, 3 May 2010 13:34:35 -0500
From: "Bauer, Karen L." <Bauer.Karen <@t> mayo.edu>
Subject: RE: [Histonet] Switching Back to Formalin
To: "Jones, Laura" <lpjones <@t> srhs-pa.org>, "Histonet (E-mail)"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <53FC421CC200C5429929EDE6C3676F30E355FA <@t> msgebe34>
Content-Type: text/plain; charset="us-ascii"
Don't dispose of it... Recycle it. Creative Waste Solutions has a nice
"Green" recycler for smaller labs, but we're using the BR Instrument for
our lab. I never imagined that we would recycle our formalin, but when
we tried out the BR, I was very impressed. We've saved so much by not
having to constantly purchase formalin and our surgery department has
quit ordering it as well. Since we have much more recycled than we can
use, surgery now gets their formalin from us.
If you can't get a recycler, we used to use D'Formalizer from Surgipath
to neutralize our formalin.
Good luck,
Karen
Karen L. Bauer HT/HTL (ASCP)
Histology Section Chief
Department of Pathology - Luther Hospital
Luther Midelfort - Mayo Health System
715-838-3205
bauer.karen <@t> mayo.edu
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jones,
Laura
Sent: Monday, May 03, 2010 1:16 PM
To: Histonet (E-mail)
Subject: [Histonet] Switching Back to Formalin
After years of working to get away from the use of Formalin in our lab,
it seems as though we are going to be bringing it back. I'm seeking
advice concerning what everyone is using to neutralize their formalin
prior to disposal. Any recommendations welcome! Thanks in advance!
Laura
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 3 May 2010 13:06:40 -0600
From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Subject: RE: [Histonet] Switching Back to Formalin
To: <bauer.karen <@t> mayo.edu>, <lpjones <@t> srhs-pa.org>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU103-W27523D9EB5481DF137469C91F20 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
I suggest Creative Waste Solutions for your Formalin recycling (actually refilering). We are a large volume lab and have been using multiple sytems (3 gallon to 20 gallon) for 5+ yrs without issue. By refiltering, you can reduce your purchase of new solution by at least 70%. There is no end point for re-use and there is no fish ordor as you get from heat distilation. Very cost effective and very user friendly.
William DeSalvo, B.S., HTL(ASCP)
> Date: Mon, 3 May 2010 13:34:35 -0500
> From: Bauer.Karen <@t> mayo.edu
> To: lpjones <@t> srhs-pa.org; Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Switching Back to Formalin
> CC:
>
> Don't dispose of it... Recycle it. Creative Waste Solutions has a nice
> "Green" recycler for smaller labs, but we're using the BR Instrument for
> our lab. I never imagined that we would recycle our formalin, but when
> we tried out the BR, I was very impressed. We've saved so much by not
> having to constantly purchase formalin and our surgery department has
> quit ordering it as well. Since we have much more recycled than we can
> use, surgery now gets their formalin from us.
>
> If you can't get a recycler, we used to use D'Formalizer from Surgipath
> to neutralize our formalin.
>
> Good luck,
>
> Karen
>
> Karen L. Bauer HT/HTL (ASCP)
> Histology Section Chief
> Department of Pathology - Luther Hospital
> Luther Midelfort - Mayo Health System
> 715-838-3205
> bauer.karen <@t> mayo.edu
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jones,
> Laura
> Sent: Monday, May 03, 2010 1:16 PM
> To: Histonet (E-mail)
> Subject: [Histonet] Switching Back to Formalin
>
> After years of working to get away from the use of Formalin in our lab,
> it seems as though we are going to be bringing it back. I'm seeking
> advice concerning what everyone is using to neutralize their formalin
> prior to disposal. Any recommendations welcome! Thanks in advance!
> Laura
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_________________________________________________________________
The New Busy think 9 to 5 is a cute idea. Combine multiple calendars with Hotmail.
http://www.windowslive.com/campaign/thenewbusy?tile=multicalendar&ocid=PID28326::T:WLMTAGL:ON:WL:en-US:WM_HMP:042010_5
------------------------------
Message: 5
Date: Mon, 3 May 2010 15:30:36 -0400
From: "jstaruk" <jstaruk <@t> masshistology.com>
Subject: [Histonet] ? about a Leica RM2155 microtome
To: "'histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <214D2296EFB74FBDA1E06471374B2D35 <@t> JimPC>
Content-Type: text/plain; charset="us-ascii"
Is anyone out there using this machine? I was offered one without the foot
switch. I was told this microtome will only work with the foot switch and
cannot be operated manually. Can someone confirm this for me?
Thanks
Jim
_______________________
James E. Staruk HT(ASCP)
www.masshistology.com
www.nehorselabs.com
------------------------------
Message: 6
Date: Mon, 03 May 2010 15:31:31 -0400
From: "Andrew Burgeson" <napoli <@t> siscom.net>
Subject: [Histonet] mouse kidney frozen sectioning
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4bdf2493.18f.2fc0.192170036 <@t> siscom.net>
Content-Type: text/plain; charset="iso-8859-1"
Having trouble with freezing artifact in the form of tiny
fissures or cracks in mouse kidney on frozen section.
Tissue is paraformaldehyde fixed and infiltrated w 70%
aqueous sucrose OCT solution.
Anyone else seen this and know how to deal with it?
Thx
------------------------------
Message: 7
Date: Mon, 03 May 2010 15:37:20 -0400
From: "Andrew Burgeson" <napoli <@t> siscom.net>
Subject: [Histonet]
C1FE5960057C084CA389CE97779062904EBD096D <@t> TCDMSG01.ad.TexasChildrensHospital.org
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4bdf25f0.10a.329b.1625663379 <@t> siscom.net>
Content-Type: text/plain; charset="iso-8859-1"
What procedures do you need to know?
------------------------------
Message: 8
Date: Mon, 3 May 2010 14:42:06 -0500
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: Re: [Histonet] ? about a Leica RM2155 microtome
To: jstaruk <jstaruk <@t> masshistology.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU0-SMTP7508B9E2FB494ABC7E1CBAEF20 <@t> phx.gbl>
Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
This is not true. You have to press the two blue buttons at the same
time on the control panel to engage the motor. One button says run
stop and the other says run enable. Remember that if you are in
continuous mode you have to press the run stop button to stop the motor.
Jack
On May 3, 2010, at 2:30 PM, "jstaruk" <jstaruk <@t> masshistology.com> wrote:
> Is anyone out there using this machine? I was offered one without
> the foot
> switch. I was told this microtome will only work with the foot
> switch and
> cannot be operated manually. Can someone confirm this for me?
>
> Thanks
>
> Jim
>
> _______________________
> James E. Staruk HT(ASCP)
> www.masshistology.com
> www.nehorselabs.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 9
Date: Mon, 3 May 2010 16:45:30 -0400
From: Drew Meyer <41dmb41 <@t> gmail.com>
Subject: [Histonet] Trichrome Help
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<q2tebcbe38a1005031345u2694c215g118c2c677a99026a <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
I'm trying to troubleshoot a problem we had today with a Trichrome stain and
I was wondering if anyone out there could help. We do Gomori Blue Collagen
Trichrome Stain... 10 minutes in Weigert's Hematoxylin and then 15 min in
the Gomori Blue Collagen stain followed by a quick change through acetic
acid, alcohol and xylene. When it's all done, there is this weird "film"
that is present over the entire surface of the slide... almost like the thin
gray film that you sometimes see when you leave slides too long in the
silver solution for a GMS. Anyways, I'm not sure what's causing it and
putting the slide in xylene or alcohol for a long time doesn't get rid of
it. It can easily be wiped off, but obviously we can't wipe the section
off. If anyone could help me narrow down what step might be causing this,
I'd appreciate it!
Thanks,
Drew
------------------------------
Message: 10
Date: Mon, 3 May 2010 18:22:22 -0400
From: "Monfils, Paul" <PMonfils <@t> Lifespan.org>
Subject: RE: [Histonet] Trichrome Help
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4EBFF65383B74D49995298C4976D1D5E06942F94 <@t> LSRIEXCH1.lsmaster.lifespan.org>
Content-Type: text/plain; charset="us-ascii"
Hi Drew,
I don't know precisely what causes this, but I have seen it happen when
using a freshly made batch of the dye solution. Also, a very fine
whitish precipitate often collects in the bottom of the bottle in which
the dye solution is stored, requiring filtration every day before using
the solution - but only when the solution is fresh. Once the solution
is a couple of weeks old, both problems disappear. Wish I had a better
explanation.
Paul
------------------------------
Message: 11
Date: Mon, 3 May 2010 15:43:55 -0700
From: "Michelle MacVeigh-Aloni" <macveigh <@t> usc.edu>
Subject: Re: [Histonet] mouse kidney frozen sectioning
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4A84D2E9DB3C4245A40E27531ABD2035 <@t> DFS66DD1>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
Hi Andrew,
You must freeze fast to avoid the needle like crystals forming from the
water during freezing. Best - freeze in OCT in liquid Nitrogen.
It gets worse if you keep changing the temp of the block. For example keep
at -80 then bring to -20 cut than bring the temp down to remove the block
from the chuck and put back at -80. Few of those will make your tissue hard
to even recognize.
Good luck
Michelle HTL
USC Keck School of Medicine
LA, CA
----- Original Message -----
From: "Andrew Burgeson" <napoli <@t> siscom.net>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, May 03, 2010 12:31 PM
Subject: [Histonet] mouse kidney frozen sectioning
> Having trouble with freezing artifact in the form of tiny
> fissures or cracks in mouse kidney on frozen section.
>
> Tissue is paraformaldehyde fixed and infiltrated w 70%
> aqueous sucrose OCT solution.
>
> Anyone else seen this and know how to deal with it?
>
> Thx
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 12
Date: Mon, 3 May 2010 16:02:07 -0700
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] ? about a Leica RM2155 microtome
To: jstaruk <jstaruk <@t> masshistology.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<g2qbdfbc2371005031602rd707ae1cu6b95ad38bb51147e <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
The RM 2155 does need a foot pedal to run. We've had ours where the
connection wasn't secure, it would stop the microtome when it wasn't
enabled. My techs use it as a manual mictotome though. The foot pedal just
sits behind the micotome. The next generation RM 2255 and RM 2265 don't need
the foot pedal to run.
On Mon, May 3, 2010 at 12:30 PM, jstaruk <jstaruk <@t> masshistology.com> wrote:
> Is anyone out there using this machine? I was offered one without the foot
> switch. I was told this microtome will only work with the foot switch and
> cannot be operated manually. Can someone confirm this for me?
>
> Thanks
>
> Jim
>
> _______________________
> James E. Staruk HT(ASCP)
> www.masshistology.com
> www.nehorselabs.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com
------------------------------
Message: 13
Date: Mon, 3 May 2010 22:53:49 EDT
From: Gervaip <@t> aol.com
Subject: [Histonet] specimen disposal
To: Histonet <@t> lists.utsouthwestern.edu, Jason.Burrill <@t> crl.com,
mdoran <@t> siumed.edu
Message-ID: <42ee4.164f7f94.3910e63d <@t> aol.com>
Content-Type: text/plain; charset="US-ASCII"
Hi, can you tell me the correct/safe way to dispose of the formalin fixed
specimens? The fixed specimens should be considered hazardous waste, but
the formalin is a chemical hazard. I think having biohazard waste company
that burns these specimens with formalin . According to MSDS we should be
able to burn the formalin and safely dispose of it that way. The large
volumes of formalin off the processors is neutralized before disposal. But we
are trying to avoid the labor intensive, hazardous job of emptying each
specimen container. That is hazardous exposure since we have no work area to
do this in safely. So, to finally get to the point, why can we not throw
all the specimens in formalin into biohazard waste bags and containers?
Pearl
"Dance like no one is watching"
------------------------------
Message: 14
Date: Tue, 4 May 2010 08:09:45 -0500
From: MariAnn.Mailhiot <@t> leica-microsystems.com
Subject: Re: [Histonet] ? about a Leica RM2155 microtome
To: Pat Laurie <foreightl <@t> gmail.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>, jstaruk
<jstaruk <@t> masshistology.com>, histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OF8461A010.7225974F-ON86257719.004814FA-86257719.00484DEE <@t> leica-microsystems.com>
Content-Type: text/plain; charset=US-ASCII
Hi All
I thought I would respond to the email. If you don't have a foot switch you
can place a dummy plug in the back where the foot pedal goes. You would
then have to get a hand pad to run the instrument.
If you have questions you are welcome to give me a call.
Kind Regards
Mari Ann Mailhiot BA HT ASCP
Application Specialist/Trainer
Leica Microsystems
Biosystems Division
Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com
Pat Laurie
<foreightl <@t> gmail.
com> To
Sent by: jstaruk <jstaruk <@t> masshistology.com>
histonet-bounces@ cc
lists.utsouthwest histonet
ern.edu <histonet <@t> lists.utsouthwestern.edu>
Subject
Re: [Histonet] ? about a Leica
05/03/2010 06:02 RM2155 microtome
PM
The RM 2155 does need a foot pedal to run. We've had ours where the
connection wasn't secure, it would stop the microtome when it wasn't
enabled. My techs use it as a manual mictotome though. The foot pedal
just
sits behind the micotome. The next generation RM 2255 and RM 2265 don't
need
the foot pedal to run.
On Mon, May 3, 2010 at 12:30 PM, jstaruk <jstaruk <@t> masshistology.com> wrote:
> Is anyone out there using this machine? I was offered one without the
foot
> switch. I was told this microtome will only work with the foot switch
and
> cannot be operated manually. Can someone confirm this for me?
>
> Thanks
>
> Jim
>
> _______________________
> James E. Staruk HT(ASCP)
> www.masshistology.com
> www.nehorselabs.com
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
______________________________________________________________________
This email has been scanned by the MessageLabs Email Security System.
For more information please visit http://www.messagelabs.com/email
______________________________________________________________________
------------------------------
Message: 15
Date: Tue, 4 May 2010 10:29:59 -0300
From: mbbarra <mbbarra <@t> uol.com.br>
Subject: [Histonet] Formalin and Kylen recycling
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <4be02157b6ad9_385fdbc1670197 <@t> weasel10.tmail>
Content-Type: text/plain; charset="utf-8"
Some help
Has anyone else use use BP instruments for? recycling. I need some
information about the subject.
Marinez Barra
Laboratory of Pathology
------------------------------
Message: 16
Date: Tue, 4 May 2010 10:56:59 -0400
From: path lab <pathology.histology <@t> gmail.com>
Subject: [Histonet] waterbath residue
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<l2x73841641005040756g4e53067fidebc997d4f820065 <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Our waterbaths leave a good amount of black residue on our paper towels when
we dump the water and wipe them out at the end of the day. Is anyone else
having this problem? It appears that the finish is gradually wearing off.
Could this be the cause of the artifact ( black specs appear to be on top
of the tissue ) that we occassionally see? Thank you in advance for your
replies.
------------------------------
Message: 17
Date: Tue, 4 May 2010 08:05:25 -0700
From: srishan <@t> mail.holyname.org
Subject: [Histonet] CBG BIOTECH RECYCLER
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF91FDD489.FB0973B9-ON85257719.00520504-88257719.0062EA43 <@t> holyname.org>
Content-Type: text/plain; charset="US-ASCII"
Hi All,
Needs some information on waste of xylene and alcohol.
We are currently using a CBG recycler in our lab. We have been told that
the clean alcohols and xylenes should not go in the recycler since it
contains paraffin.
Also the observation is, the alcohols put in the machine is percentage
wise less than what is put in. In other words the recycled alcohols are
used as 95% or less. Our cytology department does not used the recycled
alcohols or xylenes since they claim to have staining issues.
Does any one out there has developed a standard of howmany times the
alcohols and xylene can be recycled? Do you have a company pick up your
waste after a couple of recycles.
Who helps out with the waste disposal and are they poured into 55 gallon
drums and hauled away?
Any assistance will be appreciated.
Thanks
Nirmala Srishan
Holy Name Medical Center
Teaneck NJ 07666
Holy Name Medical Center is the recipient of:
Magnet Recognition for Excellence in Patient Care, American Nurses
Credentialing Center
100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern
Healthcare
Best Places to Work in New Jersey, NJBIZ
Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D.
Power
Distinguished Hospital Awards for Clinical Excellence, HealthGrades
Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care,
HealthGrades
Best in Value Award, Data Advantage, LLC
Chest Pain Center Accreditation, Society of Chest Pain Centers
Primary Stroke Center Designation, The Joint Commission and NJ Department
of Health and Human Services
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Message: 18
Date: Tue, 4 May 2010 11:12:46 -0400
From: "Donna M. Nolan" <DNolan <@t> evanhospital.com>
Subject: [Histonet] grossing qualifications
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
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<D62A58FA76A99248AEA53FB48CA559610B38180453 <@t> EVANXSMB1.evanhospital.net>
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I was asked to get opinions about grossing personnel. We have a tech with an Associate of Science in Medical Assistant with 8 credits in Anatomy and Physiology, 4 credits in Microbiology, 3 credits in Medical Terminology, and 16 credits in Medical Assistant Theory and Practicum. Would you consider her qualified to gross specimens?
Thanks,
Donna
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Message: 19
Date: Tue, 4 May 2010 10:43:30 -0500
From: "Mahoney,Janice A" <Janice.Mahoney <@t> alegent.org>
Subject: RE: [Histonet] waterbath residue
To: 'path lab' <pathology.histology <@t> gmail.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<8F0EE4144E8E2F4CA1F6B051A2E5BFEEA7C4A146 <@t> EXCHMBC2.ad.ah.local>
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Are you using pencils to mark your slides?
Jan
Omaha
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of path lab
Sent: Tuesday, May 04, 2010 9:57 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] waterbath residue
Our waterbaths leave a good amount of black residue on our paper towels when
we dump the water and wipe them out at the end of the day. Is anyone else
having this problem? It appears that the finish is gradually wearing off.
Could this be the cause of the artifact ( black specs appear to be on top
of the tissue ) that we occassionally see? Thank you in advance for your
replies.
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Message: 20
Date: Tue, 4 May 2010 09:43:58 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] CBG BIOTECH RECYCLER
To: <srishan <@t> mail.holyname.org>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B101026 <@t> server.PremierLab.local>
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Nirmala
We have the CBG recycler also. We initially started recycling both
alcohol and xylene, but we now only recycle alcohol. We ended up with
so much recycled xylene that we could not use. Also you can not use the
recycled xylene on the last xylene station in the tissue processor, or
for cleaning. It just did not work out for us for the xylene. We
rotate our xylenes and alcohols weekly so we are always putting on one
fresh absolute and one fresh xylene, there was never a place for us to
use the recycled xylene, so we ended up with lots of it. If you change
your entire tissue processor then you could put a recycled xylene in
place of your first xylene and then use fresh xylene for the last
station. We do recycle the alcohol. We get about 95% alcohol out of
the recycler - we test it for percentage of alcohol via a hydrometer and
for contamination with xylene by adding water to a small portion of it.
We only use it for 95% or less so we make up our 50%, 70% and 80%
alcohol from the recycled alcohol, these solutions do go on our tissue
processor and in the staining set up and they seem to work just fine.
To be honest we do not keep track of how many times the alcohol has been
recycled we just keep recycling it.
As for disposal we have a really cool flammable cabinet that houses a 55
gallon drum, the drum is on rollers so it can be moved easily. All of
our waste goes in there its picked up whenever it is full. We use
Source/AET Environmental to dispose of all our liquid waste.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
srishan <@t> mail.holyname.org
Sent: Tuesday, May 04, 2010 9:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CBG BIOTECH RECYCLER
Hi All,
Needs some information on waste of xylene and alcohol.
We are currently using a CBG recycler in our lab. We have been told
that
the clean alcohols and xylenes should not go in the recycler since it
contains paraffin.
Also the observation is, the alcohols put in the machine is percentage
wise less than what is put in. In other words the recycled alcohols are
used as 95% or less. Our cytology department does not used the recycled
alcohols or xylenes since they claim to have staining issues.
Does any one out there has developed a standard of howmany times the
alcohols and xylene can be recycled? Do you have a company pick up your
waste after a couple of recycles.
Who helps out with the waste disposal and are they poured into 55 gallon
drums and hauled away?
Any assistance will be appreciated.
Thanks
Nirmala Srishan
Holy Name Medical Center
Teaneck NJ 07666
Holy Name Medical Center is the recipient of:
Magnet Recognition for Excellence in Patient Care, American Nurses
Credentialing Center
100 Best Places to Work in Healthcare, Ranked Fourth Nationally by
Modern
Healthcare
Best Places to Work in New Jersey, NJBIZ
Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D.
Power
Distinguished Hospital Awards for Clinical Excellence, HealthGrades
Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care,
HealthGrades
Best in Value Award, Data Advantage, LLC
Chest Pain Center Accreditation, Society of Chest Pain Centers
Primary Stroke Center Designation, The Joint Commission and NJ
Department
of Health and Human Services
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------------------------------
Message: 21
Date: Tue, 04 May 2010 11:56:58 -0400
From: "Lynette Pavelich" <lpaveli1 <@t> hurleymc.com>
Subject: Re: [Histonet] waterbath residue
To: "path lab" <pathology.histology <@t> gmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4BE00B8A.59CD.00EE.0 <@t> hurleymc.com>
Content-Type: text/plain; charset=US-ASCII
Those black flecks could absolutely be from the waterbaths. I have yet
to find a coated waterbath that does not eventually loose its lining.
In frustration, I've had techs use a SOS pad to scrub off the lining to
finally get rid of it. Waterbath still worked, just no nice teflon
lining. I've tried returning them, but they all still went bad. Maybe
next purchase, you could go the glass liner type.
>>> path lab <pathology.histology <@t> gmail.com> 5/4/2010 10:56 AM >>>
Our waterbaths leave a good amount of black residue on our paper towels
when
we dump the water and wipe them out at the end of the day. Is anyone
else
having this problem? It appears that the finish is gradually wearing
off.
Could this be the cause of the artifact ( black specs appear to be
on top
of the tissue ) that we occassionally see? Thank you in advance for
your
replies.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 22
Date: Tue, 4 May 2010 09:39:54 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] waterbath residue
To: path lab <pathology.histology <@t> gmail.com>,
histonet <@t> lists.utsouthwestern.edu, Lynette Pavelich
<lpaveli1 <@t> hurleymc.com>
Message-ID: <456410.74283.qm <@t> web65712.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I used to glue a black X-ray film separating paraffinized sheet to the bottom of the water bath for a black background and changed it as needed.
Ren? J.
--- On Tue, 5/4/10, Lynette Pavelich <lpaveli1 <@t> hurleymc.com> wrote:
From: Lynette Pavelich <lpaveli1 <@t> hurleymc.com>
Subject: Re: [Histonet] waterbath residue
To: "path lab" <pathology.histology <@t> gmail.com>, histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, May 4, 2010, 11:56 AM
Those black flecks could absolutely be from the waterbaths.? I have yet
to find a coated waterbath that does not eventually loose its lining.
In frustration, I've had techs use a SOS pad to scrub off the lining to
finally get rid of it.? Waterbath still worked, just no nice teflon
lining.? I've tried returning them, but they all still went bad.? Maybe
next purchase, you could go the glass liner type.
>>> path lab <pathology.histology <@t> gmail.com> 5/4/2010 10:56 AM >>>
Our waterbaths leave a good amount of black residue on our paper towels
when
we dump the water and wipe them out at the end of the day.? Is anyone
else
having this problem?? It appears that the finish is gradually wearing
off.
Could this be the cause of the???artifact ( black specs appear to be
on top
of the tissue ) that we occassionally see?? Thank you in advance for
your
replies.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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End of Histonet Digest, Vol 78, Issue 4
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