[Histonet] mouse kidney frozen sectioning

[Sebree Linda A]

Cracks and fissures can also result from freezing too fast. 


Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Charles.Scouten <@t> leica-microsystems.com
Sent: Wednesday, May 05, 2010 8:34 AM
To: napoli <@t> siscom.net; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] mouse kidney frozen sectioning

Cracks and fissues is not freezing artifact, as from freezing too slow.
You are cutting the tissue too cold, and/or the blade angle is too
steep.
 
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
 
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Telephone 630 964 0501
facsimile +1 630 964 0576
www.MyNeuroLab.com <http://www.myneurolab.com/>
www.leica-microsystems.com <http://www.leica-microsystems.com/> 
 
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From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Andrew
Burgeson <napoli <@t> siscom.net>
Sent: Monday, May 03, 2010 2:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] mouse kidney frozen sectioning
 
Having trouble with freezing artifact in the form of tiny fissures or
cracks in mouse kidney on frozen section. 

Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose
OCT solution. 

Anyone else seen this and know how to deal with it? 

Thx 

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