[Histonet] RE: Feulgen/BrdU Dual labeling

[Thurby, Christina]

Hi Group,
I am re-posting my question from last week, I didn't not have many responses.  If anyone has experience with this method, please don't hesitate to e-mail or call me.

Is anyone out there doing Feulgen/BrdU Dual labeling?  I have the procedure working (Blue Feulgen kit from Scytek) first and then commercially available BrdU antibody using standard ABC detection method.  I am using a post antigen retrieval step after the Feulgen staining method is completed using Trilogy in the rice steamer.  I do not let the antigen retrieval temp exceed 95 C in the rice steamer as I have been able to reproduce staining artifact when exceeding 95 C by just 2-3 C.  I am having problems with reproducibility and labeling consistency within liver sections (1+ to 4+ zones).  Please contact me if you have experience with this procedure and can help me troubleshoot!
Thanks,
Christina Thurby
christina.thurby <@t> bms.com
Bristol Myers Squibb
812-307-2093



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