[Histonet] RE: Histonet Digest, Vol 78, Issue 2
Liebig, Tyler K.
tyler.liebig <@t> thermofisher.com
Mon May 3 07:32:20 CDT 2010
That is a funny story, I would like to see the slide that was cut from a "blade holder section" with no blade in the clamp. I have heard it can be done but never see it myself.
Though in old blade holders with loose clamps I have seen users get two blades in there at once. That didn't section to well either.
Tyler
-----Original Message-----
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Sent: Sunday, May 02, 2010 10:04 AM
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Subject: Histonet Digest, Vol 78, Issue 2
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Today's Topics:
1. Revalidation (Tench, Bill)
2. RE-hydrating air dried slides for Pap stain (Jeffrey Silverman)
3. RE: H. D., Vol 78, Issue 1, Message: 7 (Keith)
4. RE: disposable blade holder (histotech <@t> imagesbyhopper.com)
5. RE: Slide and Block Storage (histotech <@t> imagesbyhopper.com)
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Message: 1
Date: Sat, 1 May 2010 10:37:02 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] Revalidation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD02863034 <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii
Kevin, trust your judgment. You have made significant changes in your
protocol. You need to revalidate. (and frankly, most changes in a
standard protocol would require revalidation).
Bill Tench
Associate Dir. Laboratory Services
Chief, Cytology Services
Palomar Medical Center
555 E. Valley Parkway
Escondido, California 92025
Bill.Tench <@t> pph.org
Voice: 760- 739-3037
Fax: 760-739-2604
mail2.pph.org made the following annotations
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Message: 2
Date: Sat, 1 May 2010 11:43:23 -0700 (PDT)
From: Jeffrey Silverman <pathmaster <@t> yahoo.com>
Subject: [Histonet] RE-hydrating air dried slides for Pap stain
To: vrodriguez10 <@t> gmail.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID: <789166.47922.qm <@t> web111111.mail.gq1.yahoo.com>
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Hi Valerie,
It sounds like you are performing a Pap stain using saline baths to RE-hydrate air dried smears prepared at the bedside, I assume for? staining with Diff Quik stains.
The rehydration ( not dehydration) takes place in the sodium chloride baths and restores the morphology of the cells to something closer to a wet fixed state. This takes place in 30 seconds.? You then must actually fix the? now rehydrated cells in 95% alcohol. This step should be of sufficient time to completely fix the cells as one would from the start, up to 20 minutes is needed.
.
Next the cells need to be rehydrated again, just placed in a water rinse, before staining with hematoxylin and proceeding with the Pap cytoplasmic stains.
Awareness of these steps should obviate any problems.
Finally, I wouldn't judge the success or failure of the stain by the color of the slide since different tissue fluid backgrounds and amounts of lysed blood might stain differently.? Check the slide microscoipically to assess the stain.
Jeff Silverman
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Message: 3
Date: Sat, 1 May 2010 12:05:58 -0700
From: "Keith" <mikoff <@t> pacbell.net>
Subject: [Histonet] RE: H. D., Vol 78, Issue 1, Message: 7
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <NLENIJCPKPOMIABJIDGIIEEBGGAB.mikoff <@t> pacbell.net>
Content-Type: text/plain; charset="us-ascii"
Here's a copy of a procedure that provided consistently adequate results
from my old one-man lab histo days...
For your case, perhaps the slides in question were fixed differently or
improperly(?) I would pull the questionable slides, delicately rehydrate
them, run a control slide, and reprocess them with the following procedure.
In this procedure, the staining agents, dehydrants, and clearing agents and
can be modified to your facility and reagent list.
Keith M. Mikoff, HTL/HT (ASCP)
PAPANICOLAOU STAIN (PAP)
PURPOSE: The following staining procedure is to be performed only
on spray fixed slides. It is a routine stain used for any cytol-
ogy (i.e., PAP smears, FNA procedures, or specimens of a fluid
nature where cytology is required).
PROCEDURE:
1. Dehydrate slides in 2 changes of isopropyl alcohol, 5
minutes each.
2. Rinse in tap water for two minutes.
3. Stain in Harris' Hematoxylin for 2 minutes.
4. Rinse in fresh tap water for 3 minutes.
5. Place in ammonia water for 1 minute.
6. Rinse in fresh tap water for 1 minute.
7. Dehydrate in 3 changes of Isopropyl alcohol, 2 minutes each.
8. Stain in O-G 6 for one minute.
9. Dehydrate in Isopropyl alcohol for 2 changes, 2 minutes
each.
10. Stain in EA-50 for 4 minutes.
11. Place in Isopropyl alcohol, 3 changes, at 1 1/2 minutes each
12. To clear the slides, place in 3 changes of Histoclear, for 2
minutes each, and coverslip.
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Message: 4
Date: Sun, 2 May 2010 09:01:04 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: RE: [Histonet] disposable blade holder
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1729E60B0B6D456BB67E686C815CC071 <@t> hopperPC>
Content-Type: text/plain; charset="US-ASCII"
As a comical sidelight to the last sentence that Tyler wrote ...
I have a pathologist who was very upset that our cryostat didn't seem to be
working properly. I rushed up to the frozen room to assist and he said,
"well, nevermind, I got a slide and diagnosis." I looked in the cryostat
and said to him "you actually got a section and were able to render a
diagnosis?" "yes" he said. "WOW!, you must be the ONLY pathologist who is
capable of sectioning tissue without a knife ..." ;o) He sectioned the
tissue and truly had no idea there wasn't a knife in the blade holder!
:o)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Liebig,
Tyler K.
Sent: Friday, April 30, 2010 3:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] disposable blade holder
Hello Lucy,
In regards to your trouble finding an HM310 blade holder we do have one in
our refurbished pool that I could have sent to you. It is used but in
working order so we can let you have it at no charge. If you let me know
where to ship it I will have it sent.
Hope that helps, It's hard to cut without a blade holder.
Regards,
Tyler Liebig
Product Manager
Histology Instrumentation and IHC
Anatomical Pathology
Thermo Fisher Scientific
46360 Fremont Blvd
Fremont, CA 94538
USA
Office: (510) 979-5000
Mobile: (510) 299-1751
Fax: (510) 979-5239
tyler.liebig <@t> thermofisher.com<mailto:tyler.liebig <@t> thermofisher.com>
www.thermo.com<http://www.thermo.com>
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Message: 5
Date: Sun, 2 May 2010 09:04:16 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: RE: [Histonet] Slide and Block Storage
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <7180DF002734414D8FEEE9A4A4600940 <@t> hopperPC>
Content-Type: text/plain; charset="US-ASCII"
In our facility, we maintain both the blocks and slides.
We also store older blocks/slides in a climate controled an off-site
facility.
Michelle
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Kronenberger
Sent: Friday, April 30, 2010 11:00 AM
To: 'Nails, Felton'; 'Daniel Adams'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide and Block Storage
Clients fax orders for deepers and special stains which we perform and send
them out with their next package for them to read and report.
-----Original Message-----
From: Nails, Felton [mailto:flnails <@t> texaschildrens.org]
Sent: Friday, April 30, 2010 10:48 AM
To: 'Elizabeth Kronenberger'; 'Daniel Adams';
histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide and Block Storage
So what if the reporting company need to order special stains, it adds too
much time having to request the blocks or additional unstained slides.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Kronenberger
Sent: Friday, April 30, 2010 9:40 AM
To: 'Daniel Adams'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide and Block Storage
If our lab does slide preps for other clients (Technical component only), we
hold the blocks at our facility. The slides are read and reported by the
client, they hold the slides.
Liz
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Daniel Adams
Sent: Friday, April 30, 2010 12:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Slide and Block Storage
My laboratory provides professional services to some clients and technical
services to others. The question has recently come up, who should store
blocks and slides when the pathologist and histology laboratory are
different companies?
- Dan
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