[Histonet] her2 validation

Pat Laurie foreightl <@t> gmail.com
Tue Mar 30 15:26:21 CDT 2010

We left it up to our pathologist. Initially we did the 25 slide validation,
our pathologist wasn't 100% convinced that he could read them accurately, so
we did another 25, plus all of the ones we sent out which already had FISH
ran.  It ended up being almost 70 cases, and our pathologist was properly
"educated" on how he should score them.  He felt that there was excessive
pressure put on the pathologists due to having to score these slides, not
just say negative or positive.  I'll say though for the last year or so,
with about 6 her2 cases ran a day, he has been remarkably accurate.  Any
that he scores 2+ and has sent out for FISH he usually indicates if he
thinks that it will be positive or negative.  He has yet to be wrong.
Another Pathologist who I talked to who helped set up the guidelines said
that there isn't a "1 method fits all" process.  Good luck.

On Tue, Mar 30, 2010 at 7:19 AM, anita dudley <azdudley <@t> hotmail.com> wrote:

> sorry!!!  I didn't put a subject in the previous post.  wondering about how
> many slides people are using for her2 validation.  thanks
> anita dudley
> providence hospital
> mobile alabama
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Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com

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