[Histonet] mouse perfusion rate

[Joseph Saby]

All-

From previous work with rat perfusions, the flow rate was about 10 ml/minute.  If I had to guess, the equivalent flow rate for a mouse would be closer to 1-3 mls/10 minutes.  If you go 10 ml/minute, you will definitely cause blowout artefacts.

Joe Saby, BA HT




________________________________
From: Merced M Leiker <leiker <@t> buffalo.edu>
To: Charles.Scouten <@t> leica-microsystems.com; making <@t> ufl.edu; histonet <@t> lists.utsouthwestern.edu
Sent: Fri, March 19, 2010 9:21:38 AM
Subject: RE: [Histonet] mouse perfusion rate

The vasculature will leak too much and the mouse will get bloated - you'll 
see it first in either the intestines blowing up like a balloon or fluid 
coming out of the nose. Just not the same as the heart pumping when the 
mouse is alive with intact physiology and normal functioning.  Don't know 
exactly why, but that's what happens when you go too fast.  Perhaps the 
vasculature has lost its control to compensate for the pressure? I'm not a 
physiologist so I'm not sure why...maybe someone on the Histonet can answer 
that?

Regards,
Merced

--On Thursday, March 18, 2010 5:49 PM -0500 
Charles.Scouten <@t> leica-microsystems.com wrote:

>
>
> Why not?  What happens?  One would think the mammalian cardiovascular
> system could withstand physiological pressures and flow rates, at least
> for one lifetime?
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> Cordially,
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> Charles W. Scouten, Ph.D
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> Product Manager, MNL
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> Biosystems Division
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> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M
> Leiker <leiker <@t> buffalo.edu>
> Sent: Thursday, March 18, 2010 12:38 PM
> To: MKing <making <@t> ufl.edu>; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] mouse perfusion rate
>
>
>
> That may be mouse cardiac output, but I can assure you, from experience,
> you do not want to perfuse at 17ml/min.
>
> Regards,
> Merced
>
> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making <@t> ufl.edu>
> wrote:
>
>> Li,
>>
>> Mouse cardiac output seems to be about 17 ml/min (e.g.
>> www.transonic.com/mice1.shtml), you probably want to try for that to
>> keep  pressures close to physiological.
>> A syringe pump is pretty inexpensive and probably all you need.
>>
>> Mike
>>
>> ----- Original Message -----
>> From: Li Zhang < dancingwing <@t> yahoo.com>
>> Date: Wednesday, March 17, 2010 14:59
>> Subject: [Histonet] question about mouse perfusion
>> To: histonet <@t> lists.utsouthwestern.edu
>>
>> > > My question is: can anyone give me a rough idea of how fast I
>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3
>> > > min, and I suspect that it's too fast because I do observe
>> > > tissue swelling sometimes.
>>
>>
>>
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>
>
>
> Merced M Leiker
> Research Technician III
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214 USA
> leiker <@t> buffalo.edu
> 716-829-6118 (Ph)
> 716-829-2665 (Fx)
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
leiker <@t> buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)

No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.


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