[Histonet] mouse perfusion rate
saby_joseph_a <@t> yahoo.com
Sat Mar 27 19:03:53 CDT 2010
From previous work with rat perfusions, the flow rate was about 10 ml/minute. If I had to guess, the equivalent flow rate for a mouse would be closer to 1-3 mls/10 minutes. If you go 10 ml/minute, you will definitely cause blowout artefacts.
Joe Saby, BA HT
From: Merced M Leiker <leiker <@t> buffalo.edu>
To: Charles.Scouten <@t> leica-microsystems.com; making <@t> ufl.edu; histonet <@t> lists.utsouthwestern.edu
Sent: Fri, March 19, 2010 9:21:38 AM
Subject: RE: [Histonet] mouse perfusion rate
The vasculature will leak too much and the mouse will get bloated - you'll
see it first in either the intestines blowing up like a balloon or fluid
coming out of the nose. Just not the same as the heart pumping when the
mouse is alive with intact physiology and normal functioning. Don't know
exactly why, but that's what happens when you go too fast. Perhaps the
vasculature has lost its control to compensate for the pressure? I'm not a
physiologist so I'm not sure why...maybe someone on the Histonet can answer
--On Thursday, March 18, 2010 5:49 PM -0500
Charles.Scouten <@t> leica-microsystems.com wrote:
> Why not? What happens? One would think the mammalian cardiovascular
> system could withstand physiological pressures and flow rates, at least
> for one lifetime?
> Charles W. Scouten, Ph.D
> Product Manager, MNL
> Biosystems Division
> Leica Biosystems Richmond, Inc.
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> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Merced M
> Leiker <leiker <@t> buffalo.edu>
> Sent: Thursday, March 18, 2010 12:38 PM
> To: MKing <making <@t> ufl.edu>; histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] mouse perfusion rate
> That may be mouse cardiac output, but I can assure you, from experience,
> you do not want to perfuse at 17ml/min.
> --On Thursday, March 18, 2010 1:32 PM -0400 MKing < making <@t> ufl.edu>
>> Mouse cardiac output seems to be about 17 ml/min (e.g.
>> www.transonic.com/mice1.shtml), you probably want to try for that to
>> keep pressures close to physiological.
>> A syringe pump is pretty inexpensive and probably all you need.
>> ----- Original Message -----
>> From: Li Zhang < dancingwing <@t> yahoo.com>
>> Date: Wednesday, March 17, 2010 14:59
>> Subject: [Histonet] question about mouse perfusion
>> To: histonet <@t> lists.utsouthwestern.edu
>> > > My question is: can anyone give me a rough idea of how fast I
>> > > should inject ( like ml/min). I think I've tried like 30 ml in 3
>> > > min, and I suspect that it's too fast because I do observe
>> > > tissue swelling sometimes.
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
> Merced M Leiker
> Research Technician III
> Cardiovascular Medicine
> 348 Biomedical Research Building
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214 USA
> leiker <@t> buffalo.edu
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Merced M Leiker
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State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
leiker <@t> buffalo.edu
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