[Histonet] primate brain

Malika Benatti malbenatti <@t> googlemail.com
Thu Mar 25 16:30:11 CDT 2010

At what thickness do you cut your sections ?

For Human brain we cut section at room temperature moist in Molifex and cut
sections at 7 µm  for standard HE/ 14 µm  for LFB.

Not sure what is the melting point of the wax your use, but if it is around
57 oC you can safely raise the temperature of your water bath to 50 oC.

Hope this help.


On Thu, Mar 25, 2010 at 9:17 PM, Beth A Gray <gray <@t> rarc.wisc.edu> wrote:

> I have some primate brain blocks to do.  When I section the blocks the
> sections look nice.  When I put the ribbon on the water bath(  temp is 47)
> the tissue is wrinkled around the edges.  No amount of time on the water
> makes this better.  Ideas to solve this problem would be appreciated.
>  Thanks.
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