[Histonet] Re: Acetone Fixation

Lynette Pavelich lpaveli1 <@t> hurleymc.com
Thu Mar 25 12:39:59 CDT 2010


What is your goal?  A routine H&E or immuno's....etc.??

>>> "Atoska Gentry" <gentras <@t> auburn.edu> 3/25/2010 12:21 PM >>>
hello, if any of you have acetone fixation incorporated into you
frozen
section H& E staining protocol will you please advise me on
adjustments
necessary for routine staining protocol. Also, out of curiosity please
enlighten me on the purpose for post sectioning acetone fixation on
tissue samples  initially fixed in   95% ETOH/ Acetic Acid? Your
prompt
replies will be greatly appreciated. ~ Atoska

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