[Histonet] Reticulin Stain

[Malika Benatti]

Hi Alison,

Do you have a problem with all type of tissue ( Liver, Lung, Lymph Nodes,
Trephine ... ) or just Trephine specimens. ?

If you only have problems with Retic on BMT, if you use Formic Formaldehyde
Decal fluid, see if EDTA make a difference.

If you experience issue with all type of tissue. The ammoniacal silver may
be at fault, make a new batch and test it with liver or reactive Lymph Node
hopefully it will work.


In my lab we use the Gordon & Sweet Retic.  Staining protocol copy can be
found in any copy of Bancroft & Stevens or the newer version Bancroft &
Gamble, and Bancroft & Cook if you don'g have access to any of these book
you can download the staining protocol GORDON & SWEET'S
RETIC<http://library.med.utah.edu/WebPath/HISTHTML/MANUALS/RETIC.PDF>


Hope this help.

Malika

Malika Benatti

Specialist BMS
Camelia Botnar Laboratories
Histopathology Department
Great Ormond Street Hospital
London WC1N 3JH
United Kingdom


Tel:  +44 20 7405 9200 ext 5475
Fax:  +44 20 7829 7875

benatm <@t> gosh.nhs.uk


On Tue, Mar 23, 2010 at 8:35 PM, Scott, Allison D <
Allison_Scott <@t> hchd.tmc.edu> wrote:

> Hello to all in histoland.  We are having a problem with our reticulin
> stain.  It is not showing a real delination of the reticulin fibers.
> They are there but it tends to fade off.    We do the brown and hopps
> stain.  Any help would be appreciated.
>
> Allison Scott HT(ASCP)
> Histology Supervisor
> LBJ Hospital
> 5656 Kelley
> Houston, Texas 77026
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