[Histonet] Incubation of Ab more then 1 hour in autostainer

Margaryan, Naira NMargaryan <@t> childrensmemorial.org
Fri Mar 19 09:22:05 CDT 2010

Dear Histonetters,

Thanks everyone for several good advices.

I'll try all suggestions: increase volume from 600ul to 800ul, add more primary antibody step and put hot water dishes inside of machine to keep humidity as high as possible.

Hopefully it will work...........

Many thanks,

-----Original Message-----
From: Liz Chlipala [mailto:liz <@t> premierlab.com] 
Sent: Thursday, March 18, 2010 3:05 PM
To: Margaryan, Naira; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Incubation of Ab more then 1 hour in autostainer

We have done up to an hour but not longer, if I were you I would have
two antibody steps and just to add some more reagent after an hour or
1.5 hours


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Margaryan, Naira
Sent: Thursday, March 18, 2010 1:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Incubation of Ab more then 1 hour in autostainer
Importance: High

Hi Histonetters!

I have a stupid question but I Have to ask.

Does anyone perform an Incubation of Ab that required more then 1 hour
(2-3 hours) in autostainer? Does autostainer keep slides wet or slides
sometimes are getting dry? 

I know that slides should not be dry in any step of IHC.

Thank you very much!


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