[Histonet] Saffron/Eosin as a counterstain for H&E???

John Kiernan jkiernan <@t> uwo.ca
Tue Mar 16 22:05:55 CDT 2010

There is
Garvey,W (1991): Modification of the Mayer hematoxylin stain. J. Histotechnol. 14, 163-165.
The title is rather confusing. Her method included a modified composition of Mayer's haemalum (ethanol instead of chloral hydrate, and less alum), and instead of eosin Y the counterstain was a solution containing phloxin B (CI45410, Acid red 92) and saffron (which is CI 75100, Natural yellow 6). The author stated that saffron bought from a health food store was OK and much less expensive than that bought from a lab vendor.
     This counterstain was used by Rostoker et al (2001) Nephrology Dialysis Transplantation 16:513-517 in a study of intestinal pathology in glomerulonephritis.

John Kiernan
Anatomy, UWO
London, Canada
= = =
----- Original Message -----
From: sheila adey <sheila_adey <@t> hotmail.com>
Date: Tuesday, March 16, 2010 15:05
Subject: [Histonet] Saffron/Eosin as a counterstain for H&E???
To: histonet <@t> lists.utsouthwestern.edu

> Hello,
> Our pathologist said that he say H&E slides with a counterstain 
> that had some amount of saffron in it?
> Is anyone familiar with this? 
> Sheila Adey HT MLT 
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