[Histonet] Removing Yellow color from slides refixed in Bouin's solution

[Joseph Saby]

Running tap water for 10 minutes should do the trick.

Joe Saby




________________________________
From: Cynthia Pyse <cpyse <@t> x-celllab.com>
To: cscampbe <@t> uci.edu; HistoNet <histonet <@t> lists.utsouthwestern.edu>
Sent: Tue, March 16, 2010 10:52:20 AM
Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution

I just place the slides into 80% ETOH for 2 minutes.
Cindy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
cscampbe <@t> uci.edu
Sent: Monday, March 15, 2010 7:42 PM
To: HistoNet
Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's
solution

Hi Histonet,

After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it
very difficult to remove the yellow coloring. Rinses with water are not
doing the trick. Does anyone have some advice on how to bring the slides
back to a clear color so that I may proceed with the Masson Trichrome
procedure?

Thanks!
-Colin


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