[Histonet] Contamination on Processor

Bryan Hewlett bhewlett <@t> cogeco.ca
Mon Mar 15 12:21:46 CDT 2010


Kristen,
It's more likely due to the presence of lipids extracted from the tissues.
How often do you replenish/change alcohols?

Bryan

----- Original Message ----- 
From: "kristen arvidson" <arvidsonkristen <@t> yahoo.com>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, March 15, 2010 1:06 PM
Subject: [Histonet] Contamination on Processor


We are experiencing cloudiness in all of our alcohols on our processor. We 
test them before we dump them by mixing them with water and they cloud up. 
To me this says there is some clear-rite/xylene (we use both on our 
processors) getting in them somehow. I was wondering if anyone else has 
experienced this or if there is a possibility it could be some other 
contaminant or is the cloudiness normal after so many processing runs. We 
have newer processors, leica asp300s. Any help would be so much appreciated. 
thank you.



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