[Histonet] alcian blue van gieson stain

Anthony Reilly Tony_Reilly <@t> health.qld.gov.au
Sun Mar 14 18:36:42 CDT 2010

Hi Margaret
You perform the Alcian Blue first but as with Movatt's pentachrome you need to treat the sections after AB with alcoholic ammonia for an hour to convert the AB to Monastral Blue as the AB is soluable in picric acid and the Monastral blue resistant. Let me know if you need a method

Tony Reilly
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
Clinical and Statewide Services Division| QueenslandHealth
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t> health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/

>>> "Perry, Margaret" <Margaret.Perry <@t> sdstate.edu> 13/03/2010 12:30 am >>>
We want to try the double stain alcian blue Van gieson.  Do you stain for the alcian blue first and then the Van gieson?  We do both stains but I am unsure of how to put them together.
Margaret Perry
South Dakota State University

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