[Histonet] IF staining on peritoneal macrophages

Mauricio Avigdor bitesizellama <@t> gmail.com
Wed Mar 3 14:18:47 CST 2010

Hi Andrea,

Jackson lists a couple of FITC-conjugated donkey anti-rat secondaries:

Whole Donkey Anti-Rat IgG (H+L).


- F(ab')2 fragment Donkey Anti-Rat IgG (H+L).

Do you recognize which is the one you have ben using.

I have been using Serotec's STAR80F antibody. They suggest using 1:10 Normal
Mouse Serum in PBS to make the necessary dilutions. Is this something you do
with the Jackson antibodies as well? I've never heard of this technique

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