[Histonet] IF staining on peritoneal macrophages

[Mauricio Avigdor]

Hi Andrea,

Jackson lists a couple of FITC-conjugated donkey anti-rat secondaries:

712-095-150<http://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PROD&Product_Code=712-095-150>
-
Whole Donkey Anti-Rat IgG (H+L).

  712-096-150<http://www.jacksonimmuno.com/MERCHANT2/merchant.mv?Screen=PROD&Product_Code=712-096-150>

- F(ab')2 fragment Donkey Anti-Rat IgG (H+L).

Do you recognize which is the one you have ben using.

I have been using Serotec's STAR80F antibody. They suggest using 1:10 Normal
Mouse Serum in PBS to make the necessary dilutions. Is this something you do
with the Jackson antibodies as well? I've never heard of this technique
before.