[Histonet] IF staining on peritoneal macrophages

Wed Mar 3 10:09:06 CST 2010

Rat anti-mouse F480 from serotec works hwell. It is crucial that you use a secondary against rat IgG that is highly cross adsorbed to many species, including mouse. I suggest whole IgG of donkey anti-rat IgG from Jackson Immunoresearch. Just make sure to pick the version adsorbed against mouse. I would give you the catalog number except I am not at my computer.

Andrea T. Hooper