: [Histonet] frozen sections of cartilage
DeGuzman, Maria
mdeguzman <@t> lifecell.com
Tue Mar 2 11:45:20 CST 2010
Hi Kim,
Try probe on plus slides by fisherbrand for frozen samples. We use it for sectioning frozen tendon.
Maria V. De Guzman| Histology Technician I
Main 908.947.1100 Fax 908.947.1085
Direct 908.947.1482 Email mdeguzman <@t> lifecell.com
Mobile 732.688.1386 www.Lifecell.com
LifeCell Corporation | One Millennium Way | Branchburg, NJ | 08876
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, March 02, 2010 10:05 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 2
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Today's Topics:
1. RE: CD31 in rats (Hernandez, Anna)
2. CD 31 on Pig (Reuel Cornelia)
3. Re: CD 31 on Pig (Alexandra Meinl)
4. IgG4 (Martha Ward)
5. RE: Histonet Digest, Vol 76, Issue 1 (Madary, Joseph)
6. Herpes Simplex Virus Types 1 & 2 for IHC (Sebree Linda A)
7. Job opening (Kim.Donadio <@t> bhcpns.org)
8. Ab for Cartilage (Tamasi, Joseph)
9. HSV I & II (Sebree Linda A)
10. inquiry about Arthur Bolles Lee (LINDA MARGRAF)
11. Microwave processing and IHCs (Green JumpyOne)
12. RE: Microwave processing and IHCs (WILLIAM DESALVO)
13. Re: RE: [Histonet] CD31 in rats (TF)
14. RE: HSV I & II (Hoekert, W.E.J.)
15. RE: Microwave processing and IHCs (Mike Pence)
16. Re: Microwave processing and IHCs (Rene J Buesa)
17. RE: Microwave processing and IHCs (McMahon, Loralee A)
18. FW: [Histonet] Microwave processing and IHCs (Vickroy, Jim)
19. RE: inquiry about Arthur Bolles Lee (Horn, Hazel V)
----------------------------------------------------------------------
Message: 1
Date: Mon, 1 Mar 2010 13:01:03 -0500
From: "Hernandez, Anna" <Anna.Hernandez <@t> crl.com>
Subject: RE: [Histonet] CD31 in rats
To: "Annette Featherstone" <af46 <@t> buffalo.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<63EC5E7C7346EB4BA09D6DC5651E061BEA5E1E <@t> ent-pr-xch-02.na01.crl.com>
Content-Type: text/plain; charset="us-ascii"
Hello Annette,
We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog#
555025). We use on frozen sections fixed with acetone for 10 minutes
with the following steps:
1X Morphosave (Ventana) (15 min)
0.3% H202 quench(Sigma) (20 min)
Avidin and Biotin block (Vector) (15 min each)
Covance blocking reagent (25 min)
Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL
Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0
ug/mL
Covance Labeling Reagent (25 min)
Covance Dab (2 x 5 min)
(Each step has PBS washes in between and is performed at RT)
Thanks,
Anna
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Annette
Featherstone
Sent: Monday, March 01, 2010 7:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD31 in rats
Is anyone using CD31 antibody in rats, if so, what vendor and what
protocol.
thanks
Annette Featherstone
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------------------------------
Message: 2
Date: Mon, 01 Mar 2010 12:10:13 -0600
From: "Reuel Cornelia" <Reuel.Cornelia <@t> tsrh.org>
Subject: [Histonet] CD 31 on Pig
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4B8BAEA5020000C5000719EC <@t> mail.TSRH.ORG>
Content-Type: text/plain; charset=US-ASCII
Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank you.
Reuel
------------------------------
Message: 3
Date: Mon, 1 Mar 2010 19:37:31 +0100
From: Alexandra Meinl <alexandra.meinl <@t> gmail.com>
Subject: Re: [Histonet] CD 31 on Pig
To: Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org>, af46 <@t> buffalo.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<b7eff7a1003011037o3f785045l5040524c802f43b9 <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
We use the Rabbit anti-PECAM-1 (sc-1506-R, Santa Cruz) 1:100 on paraffin
sections (HIER with citrate buffer pH 6.0 required).
It works on pig and rat tissue.
Alexandra
2010/3/1 Reuel Cornelia <Reuel.Cornelia <@t> tsrh.org>
> Is anyone using CD 31 on Pigs. Where do you purchase your antibody. Thank
> you.
>
> Reuel
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
************************************************
Dr. Alexandra Meinl
Ludwig Boltzmann Institute
for Experimental and Clinical Traumatology
Austrian Cluster for Tissue Regeneration
Histology
Donaueschingenstrasse 13
1200 Vienna - Austria
Contact @ Bernhard Gottlieb University School of Dentistry,
Waehringerstr. 25a, A-1090 Vienna
tel: +43 1 4277 67026
fax: +43 1 4277 67019
email: alexandra.meinl <@t> trauma.lbg.ac.at
------------------------------
Message: 4
Date: Mon, 1 Mar 2010 13:56:17 -0500
From: "Martha Ward" <mward <@t> wfubmc.edu>
Subject: [Histonet] IgG4
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<61135F0455D33347B5AAE209B903A30433DEB713 <@t> EXCHVS2.medctr.ad.wfubmc.edu>
Content-Type: text/plain; charset="us-ascii"
Hello all,
I am looking for a lab that offers this antibody on ffpe tissues. One
of my Pathologists is interested in it and has a surgical case she has
been asked to stain for IgG4. Thanks in advance for your help!
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104
------------------------------
Message: 5
Date: Mon, 1 Mar 2010 14:02:01 -0500
From: "Madary, Joseph" <MadaryJ <@t> MedImmune.com>
Subject: [Histonet] RE: Histonet Digest, Vol 76, Issue 1
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<29A3CB81288E6F4BA2C9B3C8015A9A1301459274 <@t> MD1EV002.medimmune.com>
Content-Type: text/plain; charset="iso-8859-1"
I recall may years ago using the alcohol burner to heat my forceps and I was startled when the ice I had my blox on broke away from the pan and made a loud sound in an otherwise very quiet lab. I knocked over the alcohol burner and started a major lab fire. I used the extinguisher and all was fine but it took a while to get it all out. The biggest fear was the waste containers(good reason to recycle.
Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Mgr,
OMW, Area 4, Lab 2438
301.398.4745(vm)
301.398.6360(lab)
301.398.9745(fax)
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Monday, March 01, 2010 1:05 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 76, Issue 1
Send Histonet mailing list submissions to
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You can reach the person managing the list at
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Fire in the lab (Cheryl)
2. Fw: [Histonet] frozen sections of cartilage (Kim Merriam)
3. Pathologist Assistant Jobs (Alisha Dynan)
4. RELIA Histology Careers Bulletin 03/01/10 Are you into
Facebook? MySpace? Twitter? or Linkedin? (Pam Barker)
5. CD31 in rats (Annette Featherstone)
----------------------------------------------------------------------
Message: 1
Date: Sun, 28 Feb 2010 17:14:17 -0800 (PST)
From: Cheryl <tkngflght <@t> yahoo.com>
Subject: [Histonet] Fire in the lab
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <595933.44936.qm <@t> web50901.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Amazing that your tech had the presence of mind to do ALL of those things!
?
Used to work with a couple of old school techs back in the days when folks smoked in the lab.? One tech would embed with a xylene soaked rag to wipe the plate and a cigarette hanging out of her mouth.? She also used an open bunsen burner to keep her forceps hot....cannot tell you how many times she set fire to that rag...used her coffee to put it out most times...
?
As Louise says--in another time and dimension.
?
Cheryl
--- On Sun, 2/28/10, histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu> wrote:
From: histonet-request <@t> lists.utsouthwestern.edu <histonet-request <@t> lists.utsouthwestern.edu>
Subject: Histonet Digest, Vol 75, Issue 39
To: histonet <@t> lists.utsouthwestern.edu
Date: Sunday, February 28, 2010, 10:01 AM
Send Histonet mailing list submissions to
??? histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
??? http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
??? histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
??? histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
???1. Re: Fire in the lab (louise renton)
???2. RE: SPAM-LOW:? [Histonet] ruo antibodies (Patsy Ruegg)
???3. Fire in the lab (Jeffrey Silverman)
???4. RELIA Histology Careers Bulletin 2/28/10 Are you into
? ? ? Facebook? MySpace? Twitter? or Linkedin? (Pam Barker)
----------------------------------------------------------------------
Message: 1
Date: Sat, 27 Feb 2010 20:51:00 +0200
From: louise renton <louise.renton <@t> gmail.com>
Subject: Re: [Histonet] Fire in the lab
To: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
??? <e483362e1002271051l711a19e9y45fc3bbf47142a6a <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
hey, I once set my hair on fire in the lab...singed off my eyebrows and
burnt my nostril hair. It took several days for the burnt hair smell to get
out of my nose!!.
This too was in a time long long ago in another time dimension
On Sat, Feb 27, 2010 at 12:51 AM, Joe Nocito <jnocito <@t> satx.rr.com> wrote:
> Once upon a time in a far away land, we used to boil our embedding molds in
> boiling soapy water, over an open Bunsen burner, followed by an alcohol
> rinse then air dry. One time the fire alarm was activated and we had to
> evacuate the hospital. We were out there quit awhile. When we received the
> all clear to go back into the hospital, I was the first one back in the lab
> and the fire department was there, looking into our pot that had boiled out
> and was smoking up the lab. This wasn't the cause of the first alarm, but it
> did set off the second.
>
> Joe
> ----- Original Message ----- From: "CHRISTIE GOWAN" <christiegowan <@t> msn.com>
>
>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Friday, February 26, 2010 8:20 AM
>
> Subject: [Histonet] Fire in the lab
>
>
>
>
>
>? Dear Histonet Friends,
>
> I just wanted to share an incident we recently had with an old paraffin
> pot. One of my techs came in on Sunday to embed some tissues, went into the
> processor room and smelled something burning. He noticed our old paraffin
> pot had charred looking labels on the outside so he went over, opened the
> lid and poof!!! the pot went up in flames. The thermostat had gone haywire
> and heated the paraffin to flash point. Opening the lid gave it the oxygen
> it needed to ignite. He triggered the alarm, made the appropriate call and
> then put it out with an extinguisher. Of course it kept re-igniting because
> he could not get behind it to pull the plug. The fire dept finally was able
> to get it pulled out and unplugged. Needless to say the tech was shaken and
> the room was a mess. I applaud his courage and am not sure I would have done
> the same. There was enough xylene and alcohol on the 4 processors to cause
> quite an explosion but everything else was in a flammable cabinet. I was
> wondering if this type of thing had ever happened to anyone else?? Needless
> to say, we have de-comissioned all old paraffin pots and will order only
> those with over temp safety features. I guess I just wanted to remind
> everyone that fires can happen in the lab and do probably more often than we
> hear about. This was the first time for me and I have been in this business
> for over 20 years. Take care and be safe.
>
> Christie Gowan HT (ASCP)
>? ? ? _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
--
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
------------------------------
Message: 2
Date: Sat, 27 Feb 2010 12:47:01 -0700
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: SPAM-LOW:? [Histonet] ruo antibodies
To: "'Vickroy, Jim'" <Vickroy.Jim <@t> mhsil.com>,
??? <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <5E6E0AB0ED28430797B6300065C77E6D <@t> prueggihctechlt>
Content-Type: text/plain;??? charset="us-ascii"
Yea we were just looking at this yesterday.? There is no question on the CAP
check list about using RUO's anymore, but it is still in the discussion
section with ASR's and it is stated as you mentioned to include a disclaimer
and statement that you did try to find an IVD or ASR antibody.? Our question
was about billing the patient for RUO use.? CAP does not really address
billing issues like that, but CMS does as far as we could tell, and we have
been told that if you bill for RUO's CMS could come back and deny payment of
the claim and perhaps all bills from your lab.
We were wondering if anyone is billing patients for RUO antibodies, but of
course if you are you may not want to say so to bring attention to yourself.
Patsy Ruegg, HT(ASCP)QIHC
IHCtech
12635 Montview Blvd. Ste.215
Aurora, CO 80045
720-859-4060
fax 720-859-4110
www.ihctech.net
www.ihcrg.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, Jim
Sent: Friday, February 26, 2010 10:03 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: SPAM-LOW: [Histonet] ruo antibodies
Our new CAP checklist does not mention the use of RUO antibodies anymore.
This was under the question using ASR antibodies in the past.???I believe
the requirement was that if we wanted to use an RUO antibody we had to have
a disclaimer similar to the ASR disclaimer but we also had to have a
statement stating that we had searched for an IVD or ASR antibody.? Does
anyone know if this is still the practice or am I missing something????I do
know of course than when using either an ASR or RUO antibody we have to
establish and verify the performance.???Any thoughts about the RUO(s)?
James Vickroy BS, HT(ASCP)
Surgical? and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
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Message: 3
Date: Sat, 27 Feb 2010 12:30:35 -0800 (PST)
From: Jeffrey Silverman <pathmaster <@t> yahoo.com>
Subject: [Histonet] Fire in the lab
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <442926.94178.qm <@t> web111115.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Here's mine. Had a per diem tech who?didn't like the electric forceps warmer.? She worked over Saturdays alone and brought in an alcohol lamp to heat her forceps and then knocked it over spilling flaming alcohol all over the thermal and dispensing consoles. Then, even worse, she poured water all over both plugged- in electic appliances. We got away with just melted facade?face plates on both instruments.
?
Jeff Silverman
------------------------------
Message: 4
Date: Sat, 27 Feb 2010 22:12:50 -0500
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Histology Careers Bulletin 2/28/10 Are you
??? into??? Facebook? MySpace? Twitter? or Linkedin?
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1NlZaM-00088F-PF <@t> elasmtp-spurfowl.atl.sa.earthlink.net>
Content-Type: text/plain;??? charset="us-ascii"
Hi Histonetters!!
Facebook, Myspace, Linkedin, Twitter;? Have you joined the Social Networking
Craze?
If so then you know how much fun it can be reconnecting with old friends,
making new ones.? If you do have an account on one of these networks I would
love to connect with you.? Please shoot me back an e-mail with your id on
the site or an invitation to connect/friend/follow and I will respond right
away.
If that was like a foreign language to you and you would like some help
using any of these sites please let me know.? I would be happy to help you
join in.
I also wanted to tell you about my current job openings.? All of these jobs
are full time permanent positions and my clients offer excellent
compensation, benefits and relocation assistance.?
Need help with your resume or following up after the interview or
negotiating salary?? Give me a call or shoot me an e-mail.? I would be happy
to help out.? This is a complimentary service of RELIA of course.
Here is a list of my current open positions
HISTOLOGY/PATHOLOGY? MANAGEMENT
NY- Orange/Rockland County Histology Supervisor
MA - Cape Cod Pathology Supervisor
OR-Portland-Pathology Manager
WA-Spokane-Histology Supervisor-Hospital
CA - Central CA - Pathology Supervisor
HISTOTECHS
FL-Miami Histotechnologist needed for growing private lab
GA-Grossing Histotechnologist Night Shift Great Shift diff
MA-Boston - Immunohistochemistry Specialist
MA-Boston - Histotechs day and evening shift
NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift.
CA-Los Angeles Histotechnologist afternoon shift
PATHOLOGY/PATHOLOGIST'S ASSISTANTS
NC - Charlotte PA grad from NAACLES program required
FL- Miami Growing private lab
MARKETING AND SALES
Marketing Rep - Histo/Cyto Products Pacific NW Region
CYTOLOGY
PA-Pittsburgh Cytology Supervisor
If you or any of your friends would like more information on any of the
positions listed or help with a job search in another area please contact
me.? I would be more than happy to assist you.? You can reach me at
866-607-3542 or? <blocked::mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
Remember it never hurts to look.
Hope to hear from you soon. Thanks-Pam
Thank You!
Pam M. Barker
President
Relia
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell:? ? (407)353-5070
FAX:? ? (407)678-2788
Toll Free: (866)607-3542
e-mail:? <blocked::mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
<blocked::http://home.earthlink.net/~relia1>
http://home.earthlink.net/~relia1
<blocked::http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia
<blocked::http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia
------------------------------
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End of Histonet Digest, Vol 75, Issue 39
****************************************
------------------------------
Message: 2
Date: Mon, 1 Mar 2010 04:46:09 -0800 (PST)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: Fw: [Histonet] frozen sections of cartilage
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <836501.4247.qm <@t> web50303.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Hi Guys,
I never received a response and I was?hoping that someone would have some tips.? We?are embedding and sectioning rat and mouse knee cartilage and I?am having trouble keeping it on the slides.? Even when I do my IHC by hand, the sections are still?lifting off.? Any suggestions?
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
----- Forwarded Message ----
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Mon, February 22, 2010 2:37:58 PM
Subject: [Histonet] frozen sections of cartilage
Hi Everyone,
Any tips for keeping frozen sections of cartilage from falling off the slides?during?IHC staining?? We should not have to do HIER, but they still fall of the slides quite easily.
Kim
?Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
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------------------------------
Message: 3
Date: 1 Mar 2010 10:11:11 -0500
From: Alisha Dynan <alisha <@t> ka-recruiting.com>
Subject: [Histonet] Pathologist Assistant Jobs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1496304024.1267456271654.JavaMail.cfservice <@t> webserver53>
Content-Type: text/plain; charset="utf-8"
Dear Histonet Subscribers,
I hope you are doing well. I am a recruiter at a highly successful and well respected Healthcare recruiting firm. I help place Laboratory Professionals in permanent positions across the country and I wanted to see if you are interested in exploring other career opportunities? We are completely free of charge to candidates and and we work on quite a few laboratory positions across the country. Our clients typically assist with relocation expenses.
One particular client I am working with is one of the top hospital systems in the country. This hospital in Tennessee is looking for a Pathologist Assistant for their Pathology Department. My client is looking for someone who is PA(ASCP) certified and has at least 5 years experience as a pathologist assistant. This position has a very competitive base salary, great benefits, and an unparalleled retirement package. My client will also assist with relocation expenses.
We are also working on a number of other opportunities. See the titles below and let me know if you would like more details!
Other Current Opportunities:
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TN - Pathologist's Assistant 1st shift (must have Master's)
Midwest - Pathologist's Assistant 1st shift
Southern CA - Pathologist Assistant Supervisor
If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right fit for you please let me know who you can recommend and give me an idea of what types of positions you'd be interested in hearing about in the future. I cover the entire US and have am working on Laboratory positions at all levels. We offer a very generous referral bonus for anyone you refer to us that we place into any position across the country. To view some additional opportunities please visit our website at www.ka-recruiting.com.
Sincerely,
Alisha (Taylor) Dynan, Founder
K.A. Recruiting, Inc.
Your Partner in Healthcare Recruiting
10 Post Office Square 8th Floor SOUTH
Boston, MA 02109
P: (617) 692-2949
F: (617) 507-8009
alisha <@t> ka-recruiting.com
www.ka-recruiting.com
------------------------------
Message: 4
Date: Mon, 1 Mar 2010 10:28:38 -0500
From: "Pam Barker" <relia1 <@t> earthlink.net>
Subject: [Histonet] RELIA Histology Careers Bulletin 03/01/10 Are you
into Facebook? MySpace? Twitter? or Linkedin?
To: "'Histonet'" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E1Nm7Xz-0007hv-CR <@t> elasmtp-spurfowl.atl.sa.earthlink.net>
Content-Type: text/plain; charset="iso-8859-1"
Hello Histonetters!!
Facebook, Myspace, Linkedin, Twitter; Have you joined the Social
Networking Craze?
If so then you know how much fun it can be reconnecting with old
friends, making new ones. If you do have an account on one of these
networks I would love to connect with you. Please shoot me back an
e-mail with your id on the site or an invitation to
connect/friend/follow and I will respond right away.
If that was like a foreign language to you and you would like some help
using any of these sites please let me know. I would be happy to help
you join in.
I also wanted to tell you about my current job openings. All of these
jobs are full time permanent positions and my clients offer excellent
compensation, benefits and relocation assistance.
Need help with your resume or following up after the interview or
negotiating salary? Give me a call or shoot me an e-mail. I would be
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Here is a list of my current open positions
HISTOLOGY/PATHOLOGY MANAGEMENT
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FL-Miami Histotechnologist needed for growing private lab
GA-Grossing Histotechnologist Night Shift Great Shift diff
MA-Boston - Immunohistochemistry Specialist
MA-Boston - Histotechs day and evening shift
NY-Orange/Rockland County Brand New Lab NYS license or Elig 2nd shift.
CA-Los Angeles Histotechnologist afternoon shift
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NC - Charlotte PA grad from NAACLES program required
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If you or any of your friends would like more information on any of the
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contact me. I would be more than happy to assist you. You can reach me
at 866-607-3542 or <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
Remember it never hurts to look...
Hope to hear from you soon. Thanks-Pam
Thank You!
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President
Relia
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
Toll Free: (866)607-3542
e-mail: <mailto:relia1 <@t> earthlink.net> relia1 <@t> earthlink.net
<http://home.earthlink.net/~relia1> http://home.earthlink.net/~relia1
<http://www.myspace.com/pamatrelia> www.myspace.com/pamatrelia
<http://www.twitter.com/pamatrelia> www.twitter.com/pamatrelia
------------------------------
Message: 5
Date: Mon, 1 Mar 2010 10:44:51 -0500
From: "Annette Featherstone" <af46 <@t> buffalo.edu>
Subject: [Histonet] CD31 in rats
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000001cab956$2215a3d0$6640eb70$@edu>
Content-Type: text/plain; charset="us-ascii"
Is anyone using CD31 antibody in rats, if so, what vendor and what protocol.
thanks
Annette Featherstone
------------------------------
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
End of Histonet Digest, Vol 76, Issue 1
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To the extent this electronic communication or any of its attachments contain information that is not in the public domain, such information is considered by MedImmune to be confidential and proprietary. This communication is expected to be read and/or used only by the individual(s) for whom it is intended. If you have received this electronic communication in error, please reply to the sender advising of the error in transmission and delete the original message and any accompanying documents from your system immediately, without copying, reviewing or otherwise using them for any purpose. Thank you for your cooperation.
------------------------------
Message: 6
Date: Mon, 1 Mar 2010 13:10:34 -0600
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: [Histonet] Herpes Simplex Virus Types 1 & 2 for IHC
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<8C023B4AB999614BA4791BAEB26E27381BF7BD <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Good afternoon,
Does anyone have a "nice" HSV I & II concentrate antibody for IHC on
FFPE that they use. Biocare recently discontinued their concentrate and
we don't do enough of these to warrant buying a predilute. We'd like a
cocktail of the 2 types.
Thanks,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
------------------------------
Message: 7
Date: Mon, 1 Mar 2010 13:39:54 -0600
From: Kim.Donadio <@t> bhcpns.org
Subject: [Histonet] Job opening
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF51867145.EDF35F9B-ON862576D9.006A62BF-862576D9.006C0613 <@t> bhcpns.org>
Content-Type: text/plain; charset="US-ASCII"
Hi Histo World,
We have a Histologist position available.
Seeking someone self motivated, personable and team oriented!
Anyone interested please email me.
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
-----------------------------------------
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------------------------------
Message: 8
Date: Mon, 1 Mar 2010 15:00:26 -0500
From: "Tamasi, Joseph" <joseph.tamasi <@t> bms.com>
Subject: [Histonet] Ab for Cartilage
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<845938D0FDD6B54BAD926F60FF10A6A803E12A2DBF <@t> ushpwbmsmmp009.one.ads.bms.com>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
One of my colleagues is interested in demonstrating a marker for terminal chondrocyte differentiation in rat bone. It would be very helpful for her to know what antibodies others have used successfully for IHC. Thank you in advance for your responses.
Joe Tamasi
Senior Research Scientist
Bristol-Myers Squibb
311 Pennington-Rocky-Hill Road
Pennington, NJ 08534
609-818-3288
joseph.tamasi <@t> bms.com
________________________________
This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited.
------------------------------
Message: 9
Date: Mon, 1 Mar 2010 14:24:20 -0600
From: "Sebree Linda A" <LSebree <@t> uwhealth.org>
Subject: [Histonet] HSV I & II
To: "Histonet" <histonet <@t> pathology.swmed.edu>
Message-ID:
<8C023B4AB999614BA4791BAEB26E27381BF7BF <@t> UWHC-MAIL01.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="us-ascii"
Thanks to all who responded to my inquiry about HSV I&II however I'd
like to find it in a cocktail. I have since learned that manufacturers
can no longer premix ASR antibodies to sell . I guess I'll have to find
out if are docs need both or can make do with one.
Thanks again,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
------------------------------
Message: 10
Date: Mon, 01 Mar 2010 16:14:15 -0600
From: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>
Subject: [Histonet] inquiry about Arthur Bolles Lee
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4B8BE7D7.F783.00DA.0 <@t> childrens.com>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters
I got this request which I presume was intended for the Histonet list. Does anyone have information on this gentleman I can pass along?
Dear Linda,
I would appreciate it you could seek or make an inquiry for me. I am looking for an obituary and other biographical information about the histotechnologist Arthur Bolles Lee (1849 - 1927).
Sincerely,
Frederick H. Kasten
Johnson City, TN
Thanks...
Linda M
Histonet administrator
</pre> <span style="color: rgb(0, 160, 0); font-weight: bold;">Please consider the environment before printing this e-mail</span><br />
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------------------------------
Message: 11
Date: Mon, 1 Mar 2010 17:15:23 -0800
From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
Subject: [Histonet] Microwave processing and IHCs
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BAY107-W17E2A9630B5B1116543001AB3B0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues?
We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue.
thanks for any help!
Michelle
_________________________________________________________________
Hotmail: Free, trusted and rich email service.
http://clk.atdmt.com/GBL/go/201469228/direct/01/
------------------------------
Message: 12
Date: Mon, 1 Mar 2010 19:23:00 -0700
From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Subject: RE: [Histonet] Microwave processing and IHCs
To: <greenjumpyone <@t> hotmail.com>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU103-W140460961F87F42A3C3897913B0 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
I have used the Sakura Xpress for 5+ years and have had great success w/ all staining, w/ little to no changes. The Xpress does not use Xylene and that was a change from our conventional processing and reagents, so we did have to collect new control tissues. I say that anytime you change the process, you MUST collect new control tissue and you will ned to document. When CAP comes to visit and they see a MW processor, you will need to show them your validation and control tissue documentation. If you have the possibility of using both conventional or Microwave processd tissue, then make a modified sausage block to include both types of processing protocols, so that work can flow through the lab smoothly.
William DeSalvo, B.S., HTL(ASCP)
> From: greenjumpyone <@t> hotmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 1 Mar 2010 17:15:23 -0800
> Subject: [Histonet] Microwave processing and IHCs
>
>
> Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues?
>
> We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue.
>
> thanks for any help!
>
> Michelle
>
> _________________________________________________________________
> Hotmail: Free, trusted and rich email service.
> http://clk.atdmt.com/GBL/go/201469228/direct/01/_______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 13
Date: Tue, 2 Mar 2010 11:32:29 +0800
From: "TF" <tifei <@t> foxmail.com>
Subject: Re: RE: [Histonet] CD31 in rats
To: "Hernandez, Anna" <Anna.Hernandez <@t> crl.com>, "Annette Featherstone"
<af46 <@t> buffalo.edu>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <201003021132284371600 <@t> foxmail.com>
Content-Type: text/plain; charset="gb2312"
Hi, we use Abcam mouse-anti CD31
also acetone fixed frozen sections.
pretty well.
2010-03-02
TF
???????? Hernandez, Anna
?????????? 2010-03-02 09:50:38
???????? Annette Featherstone; histonet <@t> lists.utsouthwestern.edu
??????
?????? RE: [Histonet] CD31 in rats
Hello Annette,
We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog#
555025). We use on frozen sections fixed with acetone for 10 minutes
with the following steps:
1X Morphosave (Ventana) (15 min)
0.3% H202 quench(Sigma) (20 min)
Avidin and Biotin block (Vector) (15 min each)
Covance blocking reagent (25 min)
Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL
Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0
ug/mL
Covance Labeling Reagent (25 min)
Covance Dab (2 x 5 min)
(Each step has PBS washes in between and is performed at RT)
Thanks,
Anna
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Annette
Featherstone
Sent: Monday, March 01, 2010 7:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD31 in rats
Is anyone using CD31 antibody in rats, if so, what vendor and what
protocol.
thanks
Annette Featherstone
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 14
Date: Tue, 2 Mar 2010 10:37:02 +0100
From: "Hoekert, W.E.J." <W.E.J.Hoekert <@t> olvg.nl>
Subject: RE: [Histonet] HSV I & II
To: "Sebree Linda A" <LSebree <@t> uwhealth.org>, "Histonet"
<histonet <@t> pathology.swmed.edu>
Message-ID: <1190CB05C44B13409483514729C2FC360C0A9E <@t> PAIT42.olvg.nl>
Content-Type: text/plain; charset="iso-8859-1"
Too bad that all these people did not respond to the entire group.
We are using the one from Immunologic (polyclonal, ILP4114-C1). Is this one also not available anymore?
Willem Hoekert
OLVG, Netherlands
________________________________
Van: histonet-bounces <@t> lists.utsouthwestern.edu namens Sebree Linda A
Verzonden: ma 1-3-2010 21:24
Aan: Histonet
Onderwerp: [Histonet] HSV I & II
Thanks to all who responded to my inquiry about HSV I&II however I'd
like to find it in a cocktail. I have since learned that manufacturers
can no longer premix ASR antibodies to sell . I guess I'll have to find
out if are docs need both or can make do with one.
Thanks again,
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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------------------------------
Message: 15
Date: Tue, 2 Mar 2010 08:03:59 -0600
From: "Mike Pence" <mpence <@t> grhs.net>
Subject: RE: [Histonet] Microwave processing and IHCs
To: "Green JumpyOne" <greenjumpyone <@t> hotmail.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<661949901A768E4F9CC16D8AF8F2838C017A3D79 <@t> is-e2k3.grhs.net>
Content-Type: text/plain; charset="us-ascii"
Your control tissue needs to be processed the same as your patient
samples.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Green
JumpyOne
Sent: Monday, March 01, 2010 7:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave processing and IHCs
Does anyone here have any experience with using microwave tissue
processing and then performing IHC stains on those same tissues?
We have new microwave processors and are trying to determine if we will
need to get all new controls for our IHCs that have been microwave
processed. If yes, we will need to do validations on the controls, but
I am wondering if the MW will have any discernible effect on the
reactivity of the tissue.
thanks for any help!
Michelle
_________________________________________________________________
Hotmail: Free, trusted and rich email service.
http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________
_______________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Tue, 2 Mar 2010 06:03:58 -0800 (PST)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Microwave processing and IHCs
To: histonet <@t> lists.utsouthwestern.edu, Green JumpyOne
<greenjumpyone <@t> hotmail.com>
Message-ID: <458340.86147.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Michelle:
The key in MW tissue processing is to accelerate the process and it is very likely that the quality of the tissues will be the same as if processed in a more "traditional" way, at least that is the claim and the objective.
Having said that this does not mean that you don't have to demonstrate it with a validation process.
You should always have controls processed in the very same way as the patients' tissues so in theory you have to obtain a new set of controls processed with the your MW tissue processor.
The complication develops if you have also a "conventional" tissue processor and then you will need two sets of controls.
Ideally all your cases should be processed with a single method.
Ren? J.
--- On Mon, 3/1/10, Green JumpyOne <greenjumpyone <@t> hotmail.com> wrote:
From: Green JumpyOne <greenjumpyone <@t> hotmail.com>
Subject: [Histonet] Microwave processing and IHCs
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, March 1, 2010, 8:15 PM
Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues?
We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed.? If yes, we will need to do validations on the controls, but I am wondering if the MW will have any discernible effect on the reactivity of the tissue.
thanks for any help!
Michelle
??? ???????? ?????? ??? ?
_________________________________________________________________
Hotmail: Free, trusted and rich email service.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 17
Date: Tue, 2 Mar 2010 09:15:11 -0500
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: RE: [Histonet] Microwave processing and IHCs
To: Mike Pence <mpence <@t> grhs.net>, Green JumpyOne
<greenjumpyone <@t> hotmail.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C27AA2A01CEF31469813089E226F582E63759C12 <@t> urmcms7.urmc-sh.rochester.edu>
Content-Type: text/plain; charset="us-ascii"
In my experience I have not seen any change in the MW tissue versus the routine processing. What we have tried to do since we have so many different antibodies and control tissues is create mini sausage blocks with one piece of MW tissue and one piece of non MW tissue. Because we were not always sure what tissue were microwaved and what tissues were not.
For example one small piece of tonsil microwaved and one non microwaved embedded in the same block. That way no matter what your patient tissue sample has gone through you have all the bases covered.
It takes a long time to set up but will save you time in the end.
If you run your tissues when you validate the microwave you'll have half of the process finished.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence [mpence <@t> grhs.net]
Sent: Tuesday, March 02, 2010 9:03 AM
To: Green JumpyOne; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing and IHCs
Your control tissue needs to be processed the same as your patient
samples.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Green
JumpyOne
Sent: Monday, March 01, 2010 7:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave processing and IHCs
Does anyone here have any experience with using microwave tissue
processing and then performing IHC stains on those same tissues?
We have new microwave processors and are trying to determine if we will
need to get all new controls for our IHCs that have been microwave
processed. If yes, we will need to do validations on the controls, but
I am wondering if the MW will have any discernible effect on the
reactivity of the tissue.
thanks for any help!
Michelle
_________________________________________________________________
Hotmail: Free, trusted and rich email service.
http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________
_______________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 18
Date: Tue, 2 Mar 2010 08:37:49 -0600
From: "Vickroy, Jim" <Vickroy.Jim <@t> mhsil.com>
Subject: FW: [Histonet] Microwave processing and IHCs
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<24A4826E8EF0964D86BC5317306F58A54257908A0A <@t> mmc-mail.ad.mhsil.com>
Content-Type: text/plain; charset="us-ascii"
See below
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
-----Original Message-----
From: Vickroy, Jim
Sent: Tuesday, March 02, 2010 8:16 AM
To: 'Mike Pence'
Subject: RE: [Histonet] Microwave processing and IHCs
I understand and agree in principle. However let me ask this:
If a validation study shows that there is no difference between conventional tissue processing and microwave tissue processing in your IHC protocols then why can't you then use controls that are processed either by conventional or microwave. If we can't then are we using controls that have been decaled for tissues that have to be decaled and so forth. I have always believed if you can validate there is no appreciable difference in methods than we can use the same controls.
James Vickroy BS, HT(ASCP)
Surgical and Autopsy Pathology Technical Supervisor
Memorial Medical Center
217-788-4046
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Tuesday, March 02, 2010 8:04 AM
To: Green JumpyOne; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Microwave processing and IHCs
Your control tissue needs to be processed the same as your patient
samples.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Green
JumpyOne
Sent: Monday, March 01, 2010 7:15 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Microwave processing and IHCs
Does anyone here have any experience with using microwave tissue
processing and then performing IHC stains on those same tissues?
We have new microwave processors and are trying to determine if we will
need to get all new controls for our IHCs that have been microwave
processed. If yes, we will need to do validations on the controls, but
I am wondering if the MW will have any discernible effect on the
reactivity of the tissue.
thanks for any help!
Michelle
_________________________________________________________________
Hotmail: Free, trusted and rich email service.
http://clk.atdmt.com/GBL/go/201469228/direct/01/________________________
_______________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 19
Date: Tue, 2 Mar 2010 08:49:50 -0600
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] inquiry about Arthur Bolles Lee
To: "LINDA MARGRAF" <LINDA.MARGRAF <@t> childrens.com>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C6317D83738 <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset="us-ascii"
If you plug his name into google several links appear..
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of LINDA
MARGRAF
Sent: Monday, March 01, 2010 4:14 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] inquiry about Arthur Bolles Lee
Dear Histonetters
I got this request which I presume was intended for the Histonet list.
Does anyone have information on this gentleman I can pass along?
Dear Linda,
I would appreciate it you could seek or make an inquiry for me. I am
looking for an obituary and other biographical information about the
histotechnologist Arthur Bolles Lee (1849 - 1927).
Sincerely,
Frederick H. Kasten
Johnson City, TN
Thanks...
Linda M
Histonet administrator
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