[Histonet] CD31 in rats

[Hernandez, Anna]

Hello Annette,

We use Purified Mouse anti-Rat CD31 from BD Biosciences (Catalog#
555025).  We use on frozen sections fixed with acetone for 10 minutes
with the following steps:

1X Morphosave (Ventana) (15 min)
0.3% H202 quench(Sigma) (20 min)
Avidin and Biotin block (Vector) (15 min each)
Covance blocking reagent (25 min)
Mouse anti-Rat CD31 (25 min) at 2.0 ug/mL
Biotinylated Horse anti-Mouse IgG (Vector-BA-2001) (25 minutes) at 5.0
ug/mL
Covance Labeling Reagent (25 min)
Covance Dab (2 x 5 min)

(Each step has PBS washes in between and is performed at RT)


Thanks,

Anna 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Annette
Featherstone
Sent: Monday, March 01, 2010 7:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD31 in rats

Is anyone using CD31 antibody in rats, if so, what vendor and what
protocol.
thanks
 
Annette Featherstone
 
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