Fw: [Histonet] frozen sections of cartilage

[Kim Merriam]

Hi Guys,

I never received a response and I was hoping that someone would have some tips.  We are embedding and sectioning rat and mouse knee cartilage and I am having trouble keeping it on the slides.  Even when I do my IHC by hand, the sections are still lifting off.  Any suggestions?

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 



----- Forwarded Message ----
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Mon, February 22, 2010 2:37:58 PM
Subject: [Histonet] frozen sections of cartilage

Hi Everyone,

Any tips for keeping frozen sections of cartilage from falling off the slides during IHC staining?  We should not have to do HIER, but they still fall of the slides quite easily.

Kim
 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA 



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