[Histonet] Anybody running PIN 4 on Bond

[Dana Settembre]

Is anybody running the PIN 4 cocktail on Leica's Bond Max on human
tissue?
We are trying to bring PIN 4 into our lab.
I can't seem to get consistent staining.
I am using BioCare's PIN 4 antibodies, Mach 2 and Leica's RED Refine
detection kit.
I have used Leica's recommended times.
We have a great positive control that worked wonderfully when first 
optimized, so I thought this Leica protocol was great.
But could not duplicate results. The RED has always been fine.
 I have had BioCare replace their antibody.(nuclear staining very
light, if there)
Have changed protocols times as per BioCare's Tech Services.
I am at my wits end.
Help.
What is your protocol?
My director really wants this up and running.
Thank you,


Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA