[Histonet] Unstained Slides

[Richard Cartun]

We bake our unstains at 60 degrees C. for 30 minutes prior to filing at RT.  There is no set rule for stability.  It all depends on fixation, the nature of the protein target, and storage conditions.  I've seen absolutely spectacular immunoreactivity on unstained slides stored at RT for 20 years (and longer) and I've seen reduced immunoreactivity in unstained slides stored for as little as 4 weeks.  We will always attempt to stain unstained slides when available; however, if the lesion or tumor is negative, and there is no internal  control, you better cut fresh sections.

Richard  

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> <Rick.Garnhart <@t> memorialhealthsystem.com> 6/28/2010 11:54 AM >>>

Histoland, How  is everyone storing/filing unstained slide. And how long are they good for to use for immunohistochemistry.


Rick Garnhart HT(ASCP)
Memorial Health System
Histology Supervisor
1400 E. Boulder St.
Colorado Springs, CO 80909
Cell: 719-365-8357
Ph:  719-365-6926
Fax: 719-365-6373
rick.garnhart <@t> memorialhealthsystem.com 



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