[Histonet] Immunofloresence

Thotakura, Anil Kumar a.thotakura <@t> imperial.ac.uk
Mon Jun 28 07:11:20 CDT 2010


Dear All,

I have a problem with Immunoflorescence.(IF)

I made a monoclonal antibody, which works fine for western blot. But when I
was doing IF I had a problem. I can see the staining in my KO and WT mice, I
don't expect to see any staining in the KO mice. This same monoclonal
antibody works fine for western blot, I don't see any band in KO cell
lysates.

Can you guys please help me in this. Does antigen retrival help?

Thank you very much in advance.

Many Thanks,
Anil Kumar.





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