[Histonet] Immunofloresence

[Thotakura, Anil Kumar]

Dear All,

I have a problem with Immunoflorescence.(IF)

I made a monoclonal antibody, which works fine for western blot. But when I
was doing IF I had a problem. I can see the staining in my KO and WT mice, I
don't expect to see any staining in the KO mice. This same monoclonal
antibody works fine for western blot, I don't see any band in KO cell
lysates.

Can you guys please help me in this. Does antigen retrival help?

Thank you very much in advance.

Many Thanks,
Anil Kumar.