[Histonet] YFP staining

[James Dooley]

I trying to do staining of cytospins from CD11c Cre x rYFP. I am getting good staining using the Rabbit anti-GFP from Rockland on tissue sections that have been fixed in 4% PFA, cryoprotected. I have fixed the cytospins with 4% PFA over night and proceeded the the same staining protocol that I used on tissue section but the staining is not working. Any advice would be greatly appreciated. 

Thank you,
James L. Dooley

VIB - Autoimmune Genetics Laboratory

Campus Gasthuisberg - Department of Experimental Medicine

O&N2 bus 1026

Herestraat 49

B-3000 Leuven

Belgium

Tel:+32 16 330583

Fax:+32 16 330591