[Histonet] MMA Formulation for EXAKT Grinding

[Wahlberg, Nikki]

Hello Everyone,

I was wondering if you could help me with my MMA formulation.  I have been using a formulation that I found in a published paper.  My current embedding formulation is 80ml MMA, 20ml Dibutyl Phthalate and 3g Benzoyl Peroxide.  The samples are embedded after three days of infiltration, one change per day, with the formulation of 80ml MMA and 20ml Dibutyl Phthalate. Lately have noticed that there is a pressure build up in the vials.  I have had a few vials burst almost immediately once placed in the heated waterbath. I am filling the glass vials full with the embedding solution, capping them and then placing them in a water bath in a 37 degree oven.  They are completely polymerized by the next morning. I am also getting bubbles in the plastic when polymerized.

I have two questions: Is there any way to get rid of the bubbles in the plastic and of more concern what do you think is causing the pressure build up?

I would really appreciate any help that you can provide.

Thank you,
Nikki