[Histonet] Prepared Solutions

[Rene J Buesa]

The preparation of your solutions should be included in your SOP.
The lot numbers of your chemicals cannot be transferred to the solutions you prepare because they are a different thing.
You should determine the shelf time of your working solutions and write it in the label, along with the date they were prepared.
René J.

--- On Fri, 6/25/10, Brandi Higgins <brandihiggins <@t> gmail.com> wrote:


From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: [Histonet] Prepared Solutions
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, June 25, 2010, 12:33 PM


Hello All,

I work in a small lab where we purchase most of our chemicals already
prepared.  The only solutions we routinely prepare ourselves are our acid
alcohol and acid water for our staining procedures, Carnoy's Fixative (since
it needs to be fresh) and our 50%, 70% etc alcohol solutions for our Pap
stain and for our processing machine.  I would also like to start preparing
our 1% and 3% acetic acid solutions instead of purchasing them since I have
glacial acetic acid on hand anyway for our Carnoy's Fixative  (not that the
acetic acid acid solutions are expensive but every dollar counts, plus the
shipping charges always shock me).

My questions are
1 - in your labs, do you have a policy with instructions on how to prepare
each solution?  (eg 1 ml acetic acid diluted with water to 100 ml to prepate
1% Acetic Acid Aq and is it neccessary to say 500 ml water and 500 ml
reagent alcohol to form 50% alcohol)

2 - when you label the solutions you prepare, do you transfer the lot
numbers and expiration dates of the chemicals that were used to make the
solutions onto the new chemical bottles and use the expiration date of the
concentrated chemical as the expiration date of the prepared chemical?

Thanks in advance for all input.  Im always amazed at how fast, how many,
and how thorough/helpful the responses are!

Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet