[Histonet] New CAP question ANP.22760

[Horn, Hazel V]

So CAP answered the pathology question with a person who is a med tech
with a specialty in blood banking...

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202
 
phone   501.364.4240
fax        501.364.3155
 
visit us on the web at:    www.archildrens.org
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mark
Tarango
Sent: Friday, June 18, 2010 1:47 PM
To: McMahon, Loralee A
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] New CAP question ANP.22760

That's what I thought at first too.  It might be helpful to post this
letter
that I got from the CAP about this.  I tried to argue with them, but
this is
the answer I got.


Dear Mark,

Your questions were forwarded to me for response.



During the Audio-conference, the idea of comparing a previously stained
slide (that had used the "old" lot) to one stained with the new lot was
deemed acceptable, but not optimal. Doing a simultaneous staining using
old
and new lots, better demonstrates the performance characteristics of the
reagent.  The reason parallel staining is considered best practice is
that
all other variables, such as variations in the lot of detection reagent
or
instrument function, are eliminated from consideration when the slides
are
stained contemporaneously.



The antibody "getting weak over time" should not happen to a significant
degree if the antibody is used within its expiration date.  If the lab
is
having this kind of trouble, it should look carefully at its storage
conditions.



Demonstrating acceptable performance of the new lot, before being place
into
service, is *required* for all accredited laboratories.



To answer the last question, the key is to order the new reagent well
before
you run out of the old lot so that the parallel stain can be performed
before the old lot is consumed. One multi-tissue slide control slide
would
suffice to evaluate a primary antibody lot in most cases, which helps to
minimize the impact on the lab.



I hope that this information is helpful.  Thank you for your
participation
in the Laboratory Accreditation Program.



Sincerely,



*Kathy Passarelli, MT(ASCP)SBB*

*Technical Specialist*

*Laboratory Accreditation Program*

*College** of American** Pathologists*

*Phone: 1-(800)-323-4040 ext 7486*

*e-mail:  **kpassar <@t> cap.org* <kpassar <@t> cap.org>



On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A <
Loralee_Mcmahon <@t> urmc.rochester.edu> wrote:

> I think that CAP means that you need to save the slide that you ran
from
> the previous lot and compare it to the slide that you have stained
with the
> new lot number.  To see if they are sufficient diagnostic quality.
Not put
> both lot numbers on the machine at the same time and then compare the
> slides?   We run Dako machines and it would be tricky to put both
numbers on
> the same machine.
>
> Although this is my interpretation.
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence [
> mpence <@t> grhs.net]
> Sent: Friday, June 18, 2010 12:41 PM
> To: Ellen Yee; Laurie Colbert
>  Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] New CAP question ANP.22760
>
> I don't think I can do this with the automated system we are currently
> using. Ventana. Does any other Ventana users know if you can do this
in
> "parallel"
>
> Mike
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ellen
> Yee
> Sent: Thursday, June 17, 2010 7:21 PM
> To: Laurie Colbert
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] New CAP question ANP.22760
>
>
> Sorry, I should have included it.
>
> ANP.22760  Are new lots of antibody and detection system reagents
tested
> in parallel with old lots?  (NOTE: New lots of primary antibody and
> detection system reagents must be compared to the previous lot using
an
> appropriate panel of control tissues.)
>
> Ellen Yee
>      _____
>
>  From: Laurie Colbert [mailto:laurie.colbert <@t> huntingtonhospital.com]
> To: Ellen Yee [mailto:eyee <@t> dpmginc.com]
> Sent: Thu, 17 Jun 2010 08:47:38 -0700
> Subject: RE: [Histonet] New CAP question ANP.22760
>
> Can you give us the wording of that question/checklist item? Laurie
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ellen
> Yee
> Sent: Wednesday, June 16, 2010 10:10 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] New CAP question ANP.22760
>
> How are IHC labs complying with this question? What is considered an
> appropriate panel of control tissues? What do you stain to test your
> detection systems?
>
> Ellen Yee
>
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