[Histonet] New CAP question ANP.22760
sgoebel <@t> xbiotech.com
sgoebel <@t> xbiotech.com
Fri Jun 18 16:04:08 CDT 2010
They're taking lessons from our former president of the United St ates!! =)
Sarah Goebel, B.A., HT (ASCP)
<
Histotechnicia
XBiotech USA Inc.
<
Austin, Texas 78744
<
(512)
-------- Original Message --------
Subject: Re: [Histonet] New CAP question ANP.22760
From: [1]annigyg <@t> gmail.com
Date: Fri, June 18, 2010 1:38 pm
To: "Mark Tarango" <[2]marktaran <[3]histonet <@t> lists.u Am following this IHC/CAP thread with great interest, but I have to
ask...w English is my mother tongue but this word is new to me- simultaneous
and co abomination!
Annie.
Anyone?
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Etisa -----Original Message-----
From: Mark Tarango <[4]marktaran Sender: [5]hist Date: Fri, 18 Jun 2010 11:46:53
To: McMahon, Loralee A<[6]Loralee_Mcmahon <@t> urmc.rochester.edu>
Cc: [7]histonet <@t> lists.u tsouthwestern.edu<[8]histonet <@t> lists.utsouthwestern.edu>
Subject: Re: [Histonet] New CAP question ANP.22760
That's what I thought at first too. It might be helpful to post this
lette that I got from the CAP about this. I tried to argue with them, but
this i the answer I got.
Dear Mark,
Your questions were forwarded to me for response.
During the Audio-conference, the idea of comparing a previously
stained
slide (that had used the “old” lot) to one stained
with the deemed acceptable, but not optimal. Doing a simultaneous staining
using old and new lots, better demonstrates the performance characteristics of
the
reagent. The reason parallel staining is considered best practice is
that< detection reagent or< consideration when the slides are< The antibody "getting weak over time" should not happen to a
significant
degree if the antibody is used within its expiration date. If the lab
is having this kind of trouble, it should look carefully at its
storage
conditions.
Demonstrating acceptable performance of the new lot, before being
place int service, is *required* for all accredited laboratories.
To answer the last question, the key is to order the new reagent well
befor you run out of the old lot so that the parallel stain can be performed
before the old lot is consumed. One multi-tissue slide control slide
would< which helps to
minimize the impact on the lab.
I hope that this information is helpful. Thank you for your
participation< Sincerely,
*Kathy Passarelli, MT(ASCP)SBB*
*Technical Specialist*
*Laboratory Accreditation Program*
*College** of American** Pathologists*
*Phone: 1-(800)-323-4040 ext 7486*
*e-mail: [9]**kpassar <@t> cap.org* < On Fri, Jun 18, 2010 at 10:47 AM, McMahon, Loralee A <
[11]Loralee_Mcmahon <@t> urm > I think that CAP means that you need to save the slide that you ran
fr > the previous lot and compare it to the slide that you have stained
wit > new lot number. To see if they are sufficient diagnostic quality.
No > both lot numbers on the machine at the same time and then compare
the< both numb > the same machine.
>
> Although this is my interpretation.
>
> Loralee McMahon, HTL (ASCP)
> Immunohistochemistry Supervisor
> Strong Memorial Hospital
> Department of Surgical Pathology
> (585) 275-7210
>________________________________________
> From: [12]h > [13]histone Pence [
> [14]mpence <@t> grhs.net]
> Sent: Friday, June 18, 2010 12:41 PM
> To: Ellen Yee; Laurie Colbert
> Cc: [15]histonet <@t> l > Subject: RE: [Histonet] New CAP question ANP.22760
>
> I don't think I can do this with the automated system we are
currently > using. Ventana. Does any other Ventana users know if you can do this
i > "parallel"
>
> Mike
> -----Original Message-----
> From: [16]h > [[17]mailto:h Of Ellen
> Yee
> Sent: Thursday, June 17, 2010 7:21 PM
> To: Laurie Colbert
> Cc: [18]histonet <@t> li > Subject: RE: [Histonet] New CAP question ANP.22760
>
>
> Sorry, I should have included it.
>
> ANP.22760 Are new lots of antibody and detection system reagents
test > in parallel with old lots? (NOTE: New lots of primary antibody and
> detection system reagents must be compared to the previous lot using
a > appropriate panel of control tissues.)
>
> Ellen Yee
>_____
>
> From: Laurie Colbert
[[19]mailto:laurie.colbert <@t> huntingtonhospital.com]
> To: Ellen Yee [[20]mailto:eyee <@t> dpmginc > Sent: Thu, 17 Jun 2010 08:47:38 -0700
> Subject: RE: [Histonet] New CAP question ANP.22760
>
> Can you give us the wording of that question/checklist item? Laurie
>
> -----Original Message-----
> From: [21]h > [[22]mailto:h Of Ellen
> Yee
> Sent: Wednesday, June 16, 2010 10:10 PM
> To: [23]histonet <@t> li > Subject: [Histonet] New CAP question ANP.22760
>
> How are IHC labs complying with this question? What is considered an
> appropriate panel of control tissues? What do you stain to test your
> detection systems?
>
> Ellen Yee
>
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8. 3D"mailto://histonet@lists.utsouthwestern.=/
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28. 3D"mailto://Histonet@lists.utsouthwestern.edu"/
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