[Histonet] New CAP question ANP.22760

[BSullivan <@t> shorememorial.org]

Regarding this question. This is my take on this. You always have to check
Lot to Lot antibodies and make sure you have consistent results. We have a
ledger where our new lots are recorded when received into the lab. There is
also a place for all the necessary information about the antibody, the lot
number , open date, Q.C. date and the antibody's expiration date. Once the
antibody is opened it is Q.C'd on tissue that we have used in the past for
control material. The control is checked and it is noted if the results are
favorable or not. If there are favorable results it is put into production.

Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590


                                                                           
             "McMahon, Loralee                                             
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             u>                        Yee <eyee <@t> dpmginc.com>, Laurie      
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                                       <histonet <@t> lists.utsouthwestern.edu> 
             06/18/2010 01:47                                      Subject 
             PM                        RE: [Histonet] New CAP question     
                                       ANP.22760                           
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           
                                                                           




I think that CAP means that you need to save the slide that you ran from
the previous lot and compare it to the slide that you have stained with the
new lot number.  To see if they are sufficient diagnostic quality.  Not put
both lot numbers on the machine at the same time and then compare the
slides?   We run Dako machines and it would be tricky to put both numbers
on the same machine.

Although this is my interpretation.

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mike Pence
[mpence <@t> grhs.net]
Sent: Friday, June 18, 2010 12:41 PM
To: Ellen Yee; Laurie Colbert
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] New CAP question ANP.22760

I don't think I can do this with the automated system we are currently
using. Ventana. Does any other Ventana users know if you can do this in
"parallel"

Mike
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ellen
Yee
Sent: Thursday, June 17, 2010 7:21 PM
To: Laurie Colbert
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] New CAP question ANP.22760


Sorry, I should have included it.

ANP.22760  Are new lots of antibody and detection system reagents tested
in parallel with old lots?  (NOTE: New lots of primary antibody and
detection system reagents must be compared to the previous lot using an
appropriate panel of control tissues.)

Ellen Yee
      _____

  From: Laurie Colbert [mailto:laurie.colbert <@t> huntingtonhospital.com]
To: Ellen Yee [mailto:eyee <@t> dpmginc.com]
Sent: Thu, 17 Jun 2010 08:47:38 -0700
Subject: RE: [Histonet] New CAP question ANP.22760

Can you give us the wording of that question/checklist item? Laurie

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Ellen
Yee
Sent: Wednesday, June 16, 2010 10:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] New CAP question ANP.22760

How are IHC labs complying with this question? What is considered an
appropriate panel of control tissues? What do you stain to test your
detection systems?

Ellen Yee

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