[Histonet] Re: cryojane question

[Johnson, Teri]

To Kim,

What thickness are you using to cut these? I'm simply amazed you're having trouble keeping them on using the 4x slides and 2 flashes. Have you tried using the 1/2x or even the 1x slides? Did they wash worse using these?

Are they fixed at any time, or fresh frozen and sectioned?


To Emily,

I don't think the UV flash would affect the ISH at all.


To Liz,

We share your experience with it. The undecalcified bone and other hard tissues are very tricky to work with, and the quality of our efforts really do vary from day to day. Using this to section cartilage and decalcified bone should be pretty easy and satisfying.


Best wishes,

Teri Johnson, HT(ASCP)QIHC
Managing Director, Histology Facility
Stowers Institute for Medical Research
Kansas City, MO