[Histonet] RE: bar-coding
Maggie Allen
maggie.allen <@t> nicewareintl.com
Tue Jun 15 12:05:54 CDT 2010
Hello Carol,
Here is some information for you in regards to printing from your LIS. Please contact me at your convenience to discuss you needs in greater detail.
Niceware Healthcare offers patient safety and identification software and services to the healthcare market that are easy to deploy, affordable and fully compliant with current bar code and RFID labeling requirements. Niceware Healthcare provides high value and low maintenance software solutions to meet patient safety goals for patient identification.
The NiceForm module provides very specific identification application options in laboratory environments. For example, NiceForm drives histology cassettes and slide printing systems to uniquely identify specimen in laboratories. NiceWatch adds a separate integration layer connecting NiceLabel applications to your Laboratory Information System (LIS). While NiceForm is used for very specific identification applications, NiceWatch adds a bar code to any specimen requisition form when the LIS is incapable to output the required bar code.
The solution for Laboratory Specimen Identification provides the following benefits:
* Simplified data input and reduced error rate in data processing and label printing
* Bar code and RFID smart label printing from any Laboratory Information System (LIS)
* Easy connectivity and HL7 global messaging from multiple healthcare information systems
* High value but moderately priced
http://healthcare.nicewareintl.com/cgi-bin/site.pl?3208&dwContent_contentID=77
If you are interested in going one step further than bar-code printing and would like to learn more about LabelClinic HTS for tracking, verification and validation feel free to register for our free Webinar on June 22nd and 2:00 PM. https://nicewareintl.webex.com/mw0306lb/mywebex/default.do?service=1&siteurl=nicewareintl&nomenu=true&main_url=%2Fmc0805lb%2Fe.do%3Fsiteurl%3Dnicewareintl%26AT%3DMI%26EventID%3D118014662%26UID%3D1048843472%26Host%3D2e9af6872b3d7a5f4059%26RG%3D1%26FrameSet%3D2
Thank you,
Maggie Allen
Healthcare Business Development Manager
Niceware International, LLC
200 South Executive Drive
Suite 200
Brookfield, Wisconsin 53005
Tel (810) 629-3930
Cell (215) 200-0268
Corporate Numbers :
General: (262) 784-2456
Toll Free: (888) 894-NICE (6423)
Fax: (262) 784-2495
Technical Support: (262) 784-2466
Email: maggie.allen <@t> nicewareintl.com
www.nicewareintl.com
http://healthcare.nicewareintl.com
Maggie Allen
Healthcare Business Development Manager
Niceware International, LLC
200 South Executive Drive
Suite 200
Brookfield, Wisconsin 53005
Tel (810) 629-3930
Cell (215) 200-0268
Corporate Numbers :
General: (262) 784-2456
Toll Free: (888) 894-NICE (6423)
Fax: (262) 784-2495
Technical Support: (262) 784-2466
Email: maggie.allen <@t> nicewareintl.com
www.nicewareintl.com
http://healthcare.nicewareintl.com
FREE Webinars :
June 11, 2010, 10:30AM CST - 11:30AM CST
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, June 15, 2010 12:49 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 79, Issue 15
Send Histonet mailing list submissions to
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When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. Re: Porcine Hemagglutinating Encephalomyelitis Virus IHC
(Thomas Britten)
2. Special Stainer for Sale (Susan Michael)
3. Certification (Joyce Bidwell)
4. Hpylori (Webb, Dorothy L)
5. Thomas Crowell is out of the office. (thomas.crowell <@t> novartis.com)
6. Re: Certification (Brandi Higgins)
7. Re: Porcine Hemagglutinating Encephalomyelitis Virus IHC
(Jan Shivers)
8. RE: bar-coding (Feher, Stephen)
9. Uneven fluorescent staining (Alexia Francisca N??ez Parra)
10. Hard Tissue Forum - August 14th, 2010 in Philadelphia, PA
(Jack Ratliff)
11. Antibody Validation (Teri.Hallada <@t> midmichigan.org)
12. RE Antibody validation (Rena Fail)
13. fume hood (Brandi Higgins)
14. Re: Antibody Validation (Rene J Buesa)
15. Re: fume hood (Rene J Buesa)
16. RE: fume hood (WILLIAM DESALVO)
17. RE: Uneven fluorescent staining (Alexia Francisca N??ez Parra)
18. Re: Antibody Validation (BSullivan <@t> shorememorial.org)
19. RE: Antibody Validation (Jesus Ellin)
20. RE: Antibody Validation - long response (Liz Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Mon, 14 Jun 2010 13:28:29 -0400
From: Thomas Britten <tbritten <@t> aol.com>
Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis
Virus IHC
To: Jan Shivers <shive003 <@t> umn.edu>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <8D607429-70F6-45D5-A21A-29E62E7602EB <@t> aol.com>
Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes
What is this virus.
Sent from my iPhone
On Jun 14, 2010, at 12:21 PM, "Jan Shivers" <shive003 <@t> umn.edu> wrote:
> Does anyone know of a commercial source for unconjugated anti-
> porcine hemagglutinating encephalomyelitis virus that can be used on
> FFPE tissues?
>
> Thanks in advance,
> Jan Shivers
> Senior Scientist
> Histology/IHC/EM Section Head
> Pathology Teaching Program
> University of Minnesota
> Veterinary Diagnostic Laboratory
> 1333 Gortner Ave.
> St. Paul, MN 55108
> 612-624-7297
> shive003 <@t> umn.edu
>
> (Confidentiality Notice: This message, together with any
> attachments, is intended only for the use of the individual or
> entity to which it is addressed and may contain confidential or
> privileged information. If you think you have received this message
> in error, please advise the sender and then delete this message and
> any attachments immediately.)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 2
Date: Mon, 14 Jun 2010 13:54:35 -0400
From: Susan Michael <michaels <@t> janelia.hhmi.org>
Subject: [Histonet] Special Stainer for Sale
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <C83BE51B.22E2%michaels <@t> janelia.hhmi.org>
Content-Type: text/plain; charset="US-ASCII"
We have a Leica ST5020 special stainer that has barely been used, and we
are looking to sell it. Please let me know if this is something you may be
interested in.
Susan Michael, HTL (ASCP)
Histology/Anatomy
Janelia Farms Research Campus
19700 Helix Drive
Ashburn, VA 20147
------------------------------
Message: 3
Date: Mon, 14 Jun 2010 11:06:51 -0700 (PDT)
From: Joyce Bidwell <joyceagain <@t> att.net>
Subject: [Histonet] Certification
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <386013.49517.qm <@t> web81107.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I am in Kansas City, MO and I am in the process of getting enrolled with Indiana State University to obtain my HT certificate. Does anyone out there know if #1. It is a requirement in Missouri to have either a license or certificate to cut permanent slides? #2. Are there any other schools that offer the HT certificate training course that is a distance learning class and enrolls any time other that Fall. As it stands now I will have my prerequisits done at the end of next fall but will not be able to start the Indiana State University class until the Fall of 2011. The doctor I am working with now would like me to be able to become certified or "licensed" before then so I can cut permanent slides. Currently I am cutting MOHS tissue only.
Thanks for your input!!
------------------------------
Message: 4
Date: Mon, 14 Jun 2010 13:13:26 -0500
From: "Webb, Dorothy L" <Dorothy.L.Webb <@t> HealthPartners.Com>
Subject: [Histonet] Hpylori
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<65365F35C0F2EF4D846EC3CA73E49C43D7EFEB26AE <@t> HPEMX3.HealthPartners.int>
Content-Type: text/plain; charset="us-ascii"
Does anyone have an abundance of Hpylori tissue in blocks they would be willing to part with or trade for another control block?
We are running very low and haven't had a positive for a while!! Much appreciated!!
Dorothy Webb, HT
Regions Histology Technical Supervisor
651-254-2962
________________________________
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------------------------------
Message: 5
Date: Mon, 14 Jun 2010 14:18:44 -0400
From: thomas.crowell <@t> novartis.com
Subject: [Histonet] Thomas Crowell is out of the office.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OF1A2DF71F.47B6FB4F-ON85257742.006497C2-85257742.006497C2 <@t> ah.novartis.com>
Content-Type: text/plain; charset=US-ASCII
I will be out of the office starting 06/14/2010 and will not return until
06/15/2010.
Please contact Kelly Miner at 617-871-5122 if you have any questions
regarding clinical trial samples.
------------------------------
Message: 6
Date: Mon, 14 Jun 2010 15:21:01 -0400
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: Re: [Histonet] Certification
To: Joyce Bidwell <joyceagain <@t> att.net>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTinIYydHjo_nf3EFI4gtnA21LF3BoMWEGNsoX87n <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello,
I enrolled in the online HT program through Harford Community College in
Maryland. It is completely online. You do have to send in sample slides /
blocks / special stains, but you complete all tests and such online through
blackboard (which I also used in college, you my be familiar with that).
When I enrolled it was a rolling continuous enrollment, and suggested
completion time was 10 montsh but it was completely go at your own pace.
Hope this helps. Feel free to email me if you have any other questions.
You also need to have a certified "mentor", so hopefully your lab has an
ASCP certified tech.
Brandi Higgins, HT(ASCP)
On Mon, Jun 14, 2010 at 2:06 PM, Joyce Bidwell <joyceagain <@t> att.net> wrote:
> I am in Kansas City, MO and I am in the process of getting enrolled with
> Indiana State University to obtain my HT certificate. Does anyone out there
> know if #1. It is a requirement in Missouri to have either a license or
> certificate to cut permanent slides? #2. Are there any other schools that
> offer the HT certificate training course that is a distance learning class
> and enrolls any time other that Fall. As it stands now I will have my
> prerequisits done at the end of next fall but will not be able to start the
> Indiana State University class until the Fall of 2011. The doctor I am
> working with now would like me to be able to become certified or "licensed"
> before then so I can cut permanent slides. Currently I am cutting MOHS
> tissue only.
>
> Thanks for your input!!
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 7
Date: Mon, 14 Jun 2010 14:25:27 -0500
From: "Jan Shivers" <shive003 <@t> umn.edu>
Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis
Virus IHC
To: "Thomas Britten" <tbritten <@t> aol.com>
Cc: histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <E763A97D26104804895A359425A3D8CD <@t> auxs.umn.edu>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=response
Causal agent - a neurotropic coronavirus of young pigs; characterized by
vomiting and wasting disease and/or motor disorders (incoordination,
convulsions ) due to acute encephalomyelitis (which can lead to death within
days). Pigs are the only known natural host of the virus.
Jan Shivers
----- Original Message -----
From: "Thomas Britten" <tbritten <@t> aol.com>
To: "Jan Shivers" <shive003 <@t> umn.edu>
Cc: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, June 14, 2010 12:28 PM
Subject: Re: [Histonet] Porcine Hemagglutinating Encephalomyelitis Virus IHC
> What is this virus.
>
> Sent from my iPhone
>
> On Jun 14, 2010, at 12:21 PM, "Jan Shivers" <shive003 <@t> umn.edu> wrote:
>
>> Does anyone know of a commercial source for unconjugated anti- porcine
>> hemagglutinating encephalomyelitis virus that can be used on FFPE
>> tissues?
>>
>> Thanks in advance,
>> Jan Shivers
>> Senior Scientist
>> Histology/IHC/EM Section Head
>> Pathology Teaching Program
>> University of Minnesota
>> Veterinary Diagnostic Laboratory
>> 1333 Gortner Ave.
>> St. Paul, MN 55108
>> 612-624-7297
>> shive003 <@t> umn.edu
>>
>> (Confidentiality Notice: This message, together with any attachments, is
>> intended only for the use of the individual or entity to which it is
>> addressed and may contain confidential or privileged information. If you
>> think you have received this message in error, please advise the sender
>> and then delete this message and any attachments immediately.)
>> _______________________________________________
>> Histonet mailing list
>> Histonet <@t> lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
------------------------------
Message: 8
Date: Mon, 14 Jun 2010 17:35:59 -0400
From: "Feher, Stephen" <sfeher <@t> CMC-NH.ORG>
Subject: RE: [Histonet] bar-coding
To: "Carol Bryant" <cbrya <@t> lexclin.com>,
"Histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<73A7ED895EE0C24D9267ED814911DF1912B753FB <@t> exchange.cmc-nh.org>
Content-Type: text/plain; charset="us-ascii"
We have SoftPath 4.3 out print servers use version 4.2. We have Leica's
IPC and IPS cassette and slide printers and print all our cassettes and
slides (we print directly to the slides and do not use paper labels),
including ThinPrep slides for the Imager. Leica's application
specialists got with SoftPath and got it all working. We have no issues
and are printing slides with 2d barcodes. The bar-coded slides work
well on the BondMax IHC stainer and we are working on an interface for
the Dako Artisan Link.
It's all been working well for us. What kind of slide and cassette
printers are you using or considering using?
Steve
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Carol
Bryant
Sent: Monday, June 14, 2010 12:34 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] bar-coding
Does anyone have SoftPath 4.2? If so, do you know if it has the
capability to produce bar-coded specimen labels? I am looking into
various methods to reduce re-entry of case data and eliminate
handwriting of cassettes and slides. I know Ventana's Vantage system
can do this for histology, but we also have cytology processing in our
lab. I would like to find something that would be uniform for both
cytology and histology processing and interface with SoftPath. However,
if SoftPath could do this, even better.
Thank you in advance for any input.
Carol Bryant, CT (ASCP)
Cytology/Histology Manager
Pathology Services
Lexington Clinic
Phone (859) 258-4082
Fax (859) 258-4081
cbrya <@t> lexclin.com
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------------------------------
Message: 9
Date: Mon, 14 Jun 2010 22:09:31 +0000
From: Alexia Francisca N??ez Parra <alexia_fran <@t> hotmail.com>
Subject: [Histonet] Uneven fluorescent staining
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT129-W59EABB2CB4DF792B2ED40E93DC0 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
Hello everyone!!
The Histonet Archives have been always very helpful to me, so I decided to become a member and ask a personal question to the experts.
I am doing NeuN and BrdU double staining in the olfactory bulb, following the protocol described below. I have always had some trouble with the NeuN staining, obtaining sometimes good or no staining at all. I tried to trouble shoot with the intracardial perfusion and even bought a new NeuN antibody (Chemicon). I am having now, however, another problem. I observed an uneven NeuN and BrdU staining. There are some parts of the section that get a really good and bright staining and others that do not get any staining at all (even though they should).
Can anyone give a hint on what to review or change?
Thank you so much!!!
Alexia
1. Intracardial perfusion with cold PBS and cold 4%PFA.
2. The brains are postfixed for 4 hrs in PFA 4% at 4C and immersed in sucrose 30% ON. After that, the brains are embedded in tissue tek and store at -80.
3. Sections of 20um are obtained using a cryostat and collected using positive charged slides.
4.
Allow the slides to reach the RT
5.
Warm slides at 55C x 10'
6.
Outline slides with Immedge pen
7.
Hydrate: PBS 2 x 5', final quick wash with ddH20
8.
Soak slides in 2N HCl for 1 hr at 37C
9.
Neutralize washing the slides with 0.1M Na tretaborate buffer
pH8.5, 3 x 10'
10.
Wash with PBS 2 x 10'
11.
Wash with PBS-T 1 x 10'
12.
Block at RT with 10% of Donkey serum in PBST x 2hrs (60uL on each section)
13.
First antibody: incubate at 4C with first antibody diluited in
PBST with 2.5% Donkey serum ON (16-24hrs)
14.
Wash with PBST 4x10' at RT
15.
Secondary antibody: dilute 1:750 alexa antibodies in PBST with
2.5% Donkey serum x 2 hrs at RT
16.
Wash with PBST 1 x 10', and then with PBS 3x 10'
17.
Mount with Vectashield and seal using transparent nail polish.
_________________________________________________________________
------------------------------
Message: 10
Date: Tue, 15 Jun 2010 00:05:32 -0400
From: Jack Ratliff <ratliffjack <@t> hotmail.com>
Subject: [Histonet] Hard Tissue Forum - August 14th, 2010 in
Philadelphia, PA
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU108-W202577B3C058A0E1234D5DAEDD0 <@t> phx.gbl>
Content-Type: text/plain; charset="Windows-1252"
Attention All Hard Tissue Histologists!!!!
The Hard Tissue Committee of the National Society for Histotechnology is proud to present a one day Hard Tissue Forum. This first of its kind event will be held Saturday, August 14th, 2010 in Philadelphia, PA, from 8:00 am to 5:00 pm at the Doubletree Hotel Philadelphia. Join us as we seek to further our knowledge and understanding of the histology and analysis of bone and how this information can better serve in the diagnosis of bone related diseases and the efficacy and safety of therapeutic treatments.
Don't miss out on this one time compilation of information solely dedicated to bone as presented from both scientists and histotechnologists actively working in the field. This forum will also be a perfect opportunity to network with professionals within the "hard tissue niche". We hope to see you in August!
Check out the NSH website @ www.nsh.org to download a copy of the brochure or feel free to contact Jack L Ratliff, NSH Hard Tissue Committee Chair @ 317-281-1975 or jratliff <@t> biomimetics.com for more information.
PROGRAM AT A GLANCE
Registration Fees
Member: $119 Non Member: $159
Schedule at Glance:
7:30am-8:00am Registration & Contintental Breakfast
8:00am-9:00am Bone Biology 101 Presented by Damien Laudier,BS,HTL(ASCP),QIHC, Laudier Histology, New York, NY
9:00am-10:30am In Vivo Models for Musculoskeletal Research Presented by Timothy Ganey,PhD, Atlanta Medical Center, Atlanta, GA
10:30am-10:45am Refreshment Break
10:45am-12:15pm Concepts for Specimen Management of Samples for Decalcified Paraffin Processing Presented by Robert A. Skinner,HTL(ASCP), University of Arkansas for Medical Sciences
12:15pm-1:15pm Lunch on Your Own
1:15pm-2:45pm Resin Histology: A Practical Approach for Demonstrating Undemineralized Bone Presented by Jack L. Ratliff, BA, BioMimetic Therapeutics, Inc., Franklin, TN
2:45pm-3:00pm Refreshment Break
3:00pm-4:00pm Skeletal Analysis with MicroCT: What You Need To Know And Why You Need To Know It Presented by Daniel S. Perrien, PhD, Vanderbilt Center for Bone Biology, Nashville, TN
4:00pm-5:00pm Bone Histomorphometry Presented by Damien Laudier,BS, HTL(ASCP),QIHC, Laudier Histology, New York, NY
Travel Arrangements
Lodging
Doubletree Hotel Philadelphia
237 S. Broad Street, Philadelphia, PA 19107
Reservations via Phone: 215.893.1668
Registrants are responsible for hotel / travel arrangements. We have secured a small block of rooms at the discounted group rate of $125.00 plus applicable taxes. If you make reservations via phone please inform the agent that you are with the National Society for Histotechnology Group. Hotel Deadline is July 14, 2010.
Getting to the Forum
For directions, parking rates and airport shuttle information please visit the Doubletree's website. If you are flying the the Forum the Philadelphia International Airport is 9 miles from the hotel. Contact NSH Travel Services for best rate on air fare. Call 877.510.4747 between 8:30am-5:30pm, CT M-F or book on-line at www.nshtvl.com
Cancellation/Substitution Requests
All cancellations must be received in writing by July 14, 2010 to receive a full refund. Cancellations received after July 14, 2010 and no shows to the forum are non-refundable. Substitutions are accepted at any time. We must receive this request in writing. Individuals registering after July 14, 2010 will not be eligible for a refund.
_________________________________________________________________
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------------------------------
Message: 11
Date: Tue, 15 Jun 2010 07:55:28 -0400
From: Teri.Hallada <@t> midmichigan.org
Subject: [Histonet] Antibody Validation
To: histonet <@t> pathology.swmed.edu
Message-ID:
<8839B08E3ED7364E8CBBD53882C984D514102B37 <@t> MAILSRV01.midmichigan.net>
Content-Type: text/plain; charset=us-ascii
I am being questioned by our vendor as to why we need to validate our
automated immunostainer and image analysis instrument. They would like
documentation pertaining to the requirement of validation and the number
of specimens utilized for validation. I am requesting that each
antibody be validated on the instrument against a previously validated
instrument. Additionally, I am requesting that each new lot of antibody
be validated upon receipt against previously ran specimens. This would
also apply to the image analysis antibodies. (Her2 has been validated by
FISH.) The vendor has apparently polled users in the area and this is
not a standard protocol, therefore the request for documentation.
I think it is pretty clearly stated by CAP in the Quality Management In
Anatomic Pathology. Any other suggestions?
Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hallada <@t> midmichigan.org
989.463.1101 ext 3423
___________________________________
Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply.
------------------------------
Message: 12
Date: Tue, 15 Jun 2010 05:56:55 -0700 (PDT)
From: Rena Fail <renafail <@t> bellsouth.net>
Subject: [Histonet] RE Antibody validation
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <513753.56918.qm <@t> web180308.mail.gq1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Teri
New lots of Antibodies recieved by a lab should be validated by that laboratory. Thus proving the new lots produce similar results sa the old under the same set of circumstances. Processing, Retrevial methods, thickness of sections, techniques etc used in your laboratory contribute to the outcome.
Having the Ab validated by the vendor does not verify that it works in your laboratory with your procedures.
Rena Fail
----- Original Message ----
From: "Teri.Hallada <@t> midmichigan.org" <Teri.Hallada <@t> midmichigan.org>
To: histonet <@t> pathology.swmed.edu
Sent: Tue, June 15, 2010 7:55:28 AM
Subject: [Histonet] Antibody Validation
I am being questioned by our vendor as to why we need to validate our
automated immunostainer and image analysis instrument. They would like
documentation pertaining to the requirement of validation and the number
of specimens utilized for validation. I am requesting that each
antibody be validated on the instrument against a previously validated
instrument. Additionally, I am requesting that each new lot of antibody
be validated upon receipt against previously ran specimens. This would
also apply to the image analysis antibodies. (Her2 has been validated by
FISH.) The vendor has apparently polled users in the area and this is
not a standard protocol, therefore the request for documentation.
I think it is pretty clearly stated by CAP in the Quality Management In
Anatomic Pathology. Any other suggestions?
Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hallada <@t> midmichigan.org
989.463.1101 ext 3423
___________________________________
Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply.
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------------------------------
Message: 13
Date: Tue, 15 Jun 2010 09:51:04 -0400
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: [Histonet] fume hood
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<AANLkTimL4gBsGE6C_gbNGAa1qcDfWTVQQ4UEYXxaH2WR <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1
Hello,
Our hospital is doing some renovation and we need to look into new fume
hoods for our new location. Currently we have one fume hood over our
grossing area, and one fume hood in our coverslipping area (two different
rooms). The hospital wants to put our grossing room and histo/cyto rooms
together. I am still going to need two separate hoods. Does anyone have
any experience/knowledge/input about fume hoods? I'm trying to look into
the ductless ones, although I imagine changing the filters will end up being
more expensive over time (I have no idea what would be involved in running a
duct/vent). Also I have seen a benchtop downdraft type that sucks the air
down, and does not have a top. It is advertised as being good for xylene.
Does anyone use this in their coverslipping area? Any input would be
greatly appreciated. I'm pretty clueless on the whole issue. I want to
make sure that what I get will be safe for me and my coworker as we will be
spending most of our day in this room. Any input is appreciated! Thank
You!
Brandi Higgins, BS, HT(ASCP)
------------------------------
Message: 14
Date: Tue, 15 Jun 2010 07:49:34 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Antibody Validation
To: histonet <@t> pathology.swmed.edu, Teri.Hallada <@t> midmichigan.org
Message-ID: <99966.29247.qm <@t> web65716.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Teri:
You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high quality of your work to obtaining the most accurate work for patients' sake.
There is where the difference resides. Ignore your vendor and keep validating your protocols.
René J.
--- On Tue, 6/15/10, Teri.Hallada <@t> midmichigan.org <Teri.Hallada <@t> midmichigan.org> wrote:
From: Teri.Hallada <@t> midmichigan.org <Teri.Hallada <@t> midmichigan.org>
Subject: [Histonet] Antibody Validation
To: histonet <@t> pathology.swmed.edu
Date: Tuesday, June 15, 2010, 7:55 AM
I am being questioned by our vendor as to why we need to validate our
automated immunostainer and image analysis instrument. They would like
documentation pertaining to the requirement of validation and the number
of specimens utilized for validation. I am requesting that each
antibody be validated on the instrument against a previously validated
instrument. Additionally, I am requesting that each new lot of antibody
be validated upon receipt against previously ran specimens. This would
also apply to the image analysis antibodies. (Her2 has been validated by
FISH.) The vendor has apparently polled users in the area and this is
not a standard protocol, therefore the request for documentation.
I think it is pretty clearly stated by CAP in the Quality Management In
Anatomic Pathology. Any other suggestions?
Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hallada <@t> midmichigan.org
989.463.1101 ext 3423
___________________________________
Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 15
Date: Tue, 15 Jun 2010 07:53:03 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] fume hood
To: histonet <@t> lists.utsouthwestern.edu, Brandi Higgins
<brandihiggins <@t> gmail.com>
Message-ID: <422333.48491.qm <@t> web65705.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
Over the counter fumes hood with filters are very effcicient and cheaper than running the vent ducts, especially in a large building.
They also have the advantage that they can be moved to another place if necessary.
Those without a cover are not that efficient.
René J.
--- On Tue, 6/15/10, Brandi Higgins <brandihiggins <@t> gmail.com> wrote:
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: [Histonet] fume hood
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, June 15, 2010, 9:51 AM
Hello,
Our hospital is doing some renovation and we need to look into new fume
hoods for our new location. Currently we have one fume hood over our
grossing area, and one fume hood in our coverslipping area (two different
rooms). The hospital wants to put our grossing room and histo/cyto rooms
together. I am still going to need two separate hoods. Does anyone have
any experience/knowledge/input about fume hoods? I'm trying to look into
the ductless ones, although I imagine changing the filters will end up being
more expensive over time (I have no idea what would be involved in running a
duct/vent). Also I have seen a benchtop downdraft type that sucks the air
down, and does not have a top. It is advertised as being good for xylene.
Does anyone use this in their coverslipping area? Any input would be
greatly appreciated. I'm pretty clueless on the whole issue. I want to
make sure that what I get will be safe for me and my coworker as we will be
spending most of our day in this room. Any input is appreciated! Thank
You!
Brandi Higgins, BS, HT(ASCP)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 16
Date: Tue, 15 Jun 2010 09:19:46 -0600
From: WILLIAM DESALVO <wdesalvo.cac <@t> hotmail.com>
Subject: RE: [Histonet] fume hood
To: <brandihiggins <@t> gmail.com>, histonet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <BLU103-W7BCA6C1D29FDF162855EB91DD0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
There are two units we use in our labs and I suggest looking into the Mopec BF600 Downdraft Workcell. This works great for H&E/FS stain lines and other areas w/ open containers of chemicals. I also suggest considering the Labconco Protector XVS Ventilation Station for counter top hood with easy access, the unit is connected to outside air extraction. There are many other options and you should do a search on the internet for more options.
William DeSalvo, B.S., HTL(ASCP)
Production Manager, Sonora Quest laboratories
Chair, NSH QCC
> Date: Tue, 15 Jun 2010 09:51:04 -0400
> From: brandihiggins <@t> gmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] fume hood
>
> Hello,
>
> Our hospital is doing some renovation and we need to look into new fume
> hoods for our new location. Currently we have one fume hood over our
> grossing area, and one fume hood in our coverslipping area (two different
> rooms). The hospital wants to put our grossing room and histo/cyto rooms
> together. I am still going to need two separate hoods. Does anyone have
> any experience/knowledge/input about fume hoods? I'm trying to look into
> the ductless ones, although I imagine changing the filters will end up being
> more expensive over time (I have no idea what would be involved in running a
> duct/vent). Also I have seen a benchtop downdraft type that sucks the air
> down, and does not have a top. It is advertised as being good for xylene.
> Does anyone use this in their coverslipping area? Any input would be
> greatly appreciated. I'm pretty clueless on the whole issue. I want to
> make sure that what I get will be safe for me and my coworker as we will be
> spending most of our day in this room. Any input is appreciated! Thank
> You!
>
> Brandi Higgins, BS, HT(ASCP)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_________________________________________________________________
The New Busy is not the old busy. Search, chat and e-mail from your inbox.
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------------------------------
Message: 17
Date: Tue, 15 Jun 2010 15:28:42 +0000
From: Alexia Francisca N??ez Parra <alexia_fran <@t> hotmail.com>
Subject: RE: [Histonet] Uneven fluorescent staining
To: lita de foro <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <SNT129-W28844ED007E3CE162AF4E893DD0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"
Thank you so much for your answer Anatoli!
- I tried the citrate buffer boiling system and I obtained the same unreliable results with NeuN. Some animals showed a great NeuN staining and others did not. I really thought at that point that my perfusion was the problem and I switched from making my own PFA4% to buying a 16%PFA from Electron Microscopy Sciences, which I think solved the problem. Now I'm obtaining a good NeuN staining but only in some regions of the tissue.
- I am using the Rat monoclonal from Abcam (1:250)
- I'll consider the new method using Edu labeling for new experiment and will try the ProLong Gold antifade, thank you very much for the advice.
Alexia Nunez
University of Maryland, College Park
> Subject: RE: [Histonet] Uneven fluorescent staining
> Date: Tue, 15 Jun 2010 10:26:32 -0400
> From: AGleiberman <@t> cbiolabs.com
> To: alexia_fran <@t> hotmail.com
>
> Alexia,
> 1. HCl treatment could be sometimes problematic - many epitopes are quite sensitive to it, so your NeuN antibody will work not in optimal conditions. You can replace acid treatment with citrate buffer boiling - the same treatment that is used for paraffin sections. It will denature DNA as effectively as HCl treatment.
> 2. You did not mention what antibody you are using for BrdU - the best are rat monoclonal from Abcam (much better than any mouse monoclonal or sheep or goat polyclonal I have tested) - and you can easy combine them with mouse monoclonal against NeuN (I believe, only mouse anti-NeuN exist).
> 3. For future experiments I recommend switch from BrdU labeling to EdU labeling (Click-iT® EdU Alexa Fluor® 488 HCS Assay from Invitrogen) - this technique is more sensitive for studying DNA synthesis than BrdU, staining is faster (30min), does not involve neither denaturation step nor antibody and can be easily combined with any other marker staining. To do antigen staining after EdU visualization, that is simple chemical reaction, you need wash thoroughly your slides and perform your antibody staining. Additional benefit is - much better structure of nuclei because denaturation step sometimes affects them. In addition, because of high sensitivity you can decrease concentration of nucleotide substitute (EdU) 5-10 times in comparison with BrdU that can be crucial for long-term experiments because all these nucleotide analogues (especially BrdU) can affect cell fate in long run.
> 4. Finally, I recommend to replace Vectashield with ProLong Gold antifade (Invitrogen) with or without DAPI. It is better for fluorochrome preservation and it will harden overnight - you don't need any nail polish
>
> Anatoli Gleiberman, PhD
> Director of Histopathology
> Cleveland Biolabs, Inc
> 73 High Street
> Buffalo, NY 14203
> phone:716-849-6810 ext.354
> fax:716-849-6817
> e-mail: AGleiberman <@t> cbiolabs.com
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Alexia Francisca Núñez Parra
> Sent: Monday, June 14, 2010 6:10 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Uneven fluorescent staining
>
>
> Hello everyone!!
> The Histonet Archives have been always very helpful to me, so I decided to become a member and ask a personal question to the experts.
>
> I am doing NeuN and BrdU double staining in the olfactory bulb, following the protocol described below. I have always had some trouble with the NeuN staining, obtaining sometimes good or no staining at all. I tried to trouble shoot with the intracardial perfusion and even bought a new NeuN antibody (Chemicon). I am having now, however, another problem. I observed an uneven NeuN and BrdU staining. There are some parts of the section that get a really good and bright staining and others that do not get any staining at all (even though they should).
>
> Can anyone give a hint on what to review or change?
>
> Thank you so much!!!
>
> Alexia
>
> 1. Intracardial perfusion with cold PBS and cold 4%PFA.
> 2. The brains are postfixed for 4 hrs in PFA 4% at 4C and immersed in sucrose 30% ON. After that, the brains are embedded in tissue tek and store at -80.
> 3. Sections of 20um are obtained using a cryostat and collected using positive charged slides.
>
>
>
>
>
>
>
>
>
>
>
>
>
>
> 4.
> Allow the slides to reach the RT
>
>
> 5.
> Warm slides at 55C x 10'
>
> 6.
> Outline slides with Immedge pen
>
> 7.
> Hydrate: PBS 2 x 5', final quick wash with ddH20
>
> 8.
> Soak slides in 2N HCl for 1 hr at 37C
>
> 9.
> Neutralize washing the slides with 0.1M Na tretaborate buffer
> pH8.5, 3 x 10'
>
> 10.
> Wash with PBS 2 x 10'
>
> 11.
> Wash with PBS-T 1 x 10'
>
>
>
> 12.
> Block at RT with 10% of Donkey serum in PBST x 2hrs (60uL on each section)
>
> 13.
> First antibody: incubate at 4C with first antibody diluited in
> PBST with 2.5% Donkey serum ON (16-24hrs)
>
> 14.
> Wash with PBST 4x10' at RT
>
> 15.
> Secondary antibody: dilute 1:750 alexa antibodies in PBST with
> 2.5% Donkey serum x 2 hrs at RT
>
> 16.
> Wash with PBST 1 x 10', and then with PBS 3x 10'
>
>
>
> 17.
> Mount with Vectashield and seal using transparent nail polish.
>
>
>
>
>
> _________________________________________________________________
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information.
_________________________________________________________________
------------------------------
Message: 18
Date: Tue, 15 Jun 2010 11:49:28 -0400
From: BSullivan <@t> shorememorial.org
Subject: Re: [Histonet] Antibody Validation
To: Rene J Buesa <rjbuesa <@t> yahoo.com>
Cc: Teri.Hallada <@t> midmichigan.org,
histonet-bounces <@t> lists.utsouthwestern.edu,
histonet <@t> pathology.swmed.edu
Message-ID:
<OF3D62BE1C.9FF87DB3-ON85257743.00569056-85257743.00571F2C <@t> shorememorial.org>
Content-Type: text/plain; charset=ISO-8859-1
I agree with Rene. All lot to lot and new antibodies need to be checked for
consistency. This is part of your validation process. This even holds true
for pre-dilutes which are already tested by the manufacturer for optimum
results.
Beatrice Sullivan, HT(A.S.C.P.) HTL , AAS, CLSP(N.C.A.)
AP Supervisor
Shore Memorial Hospital
609-653-3590
Rene J Buesa
<rjbuesa <@t> yahoo.co
m> To
Sent by: histonet <@t> pathology.swmed.edu,
histonet-bounces@ Teri.Hallada <@t> midmichigan.org
lists.utsouthwest cc
ern.edu
Subject
Re: [Histonet] Antibody Validation
06/15/2010 10:49
AM
Teri:
You are right about the validations you propose although I am not surprised
that your vendor does not think it is necessary. They are in the business
of selling and you are in the business of assuring the high quality of your
work to obtaining the most accurate work for patients' sake.
There is where the difference resides. Ignore your vendor and keep
validating your protocols.
René J.
--- On Tue, 6/15/10, Teri.Hallada <@t> midmichigan.org
<Teri.Hallada <@t> midmichigan.org> wrote:
From: Teri.Hallada <@t> midmichigan.org <Teri.Hallada <@t> midmichigan.org>
Subject: [Histonet] Antibody Validation
To: histonet <@t> pathology.swmed.edu
Date: Tuesday, June 15, 2010, 7:55 AM
I am being questioned by our vendor as to why we need to validate our
automated immunostainer and image analysis instrument. They would like
documentation pertaining to the requirement of validation and the number
of specimens utilized for validation. I am requesting that each
antibody be validated on the instrument against a previously validated
instrument. Additionally, I am requesting that each new lot of antibody
be validated upon receipt against previously ran specimens. This would
also apply to the image analysis antibodies. (Her2 has been validated by
FISH.) The vendor has apparently polled users in the area and this is
not a standard protocol, therefore the request for documentation.
I think it is pretty clearly stated by CAP in the Quality Management In
Anatomic Pathology. Any other suggestions?
Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hallada <@t> midmichigan.org
989.463.1101 ext 3423
___________________________________
Please note that this email message and any attachments may contain
privileged and confidential information that is protected against use or
disclosure under federal and state law. The information is intended only
for the personal and confidential use of the intended recipient. If the
reader of this message is not the intended recipient or the employee or
agent responsible for delivering it to the intended recipient, you are
hereby notified that you have received this information in error and that
any review, dissemination, distribution, copying or action taken in
reliance on the contents of this communication is strictly prohibited. If
you have received this email in error, please advise by immediate reply.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 19
Date: Tue, 15 Jun 2010 08:57:21 -0700
From: "Jesus Ellin" <JEllin <@t> yumaregional.org>
Subject: RE: [Histonet] Antibody Validation
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,
<histonet <@t> pathology.swmed.edu>, <Teri.Hallada <@t> midmichigan.org>
Message-ID:
<29BE166A2CF48D459853F8EC57CD37E8021C63F5 <@t> EXCHANGECLUSTER.yumaregional.local>
Content-Type: text/plain; charset="us-ascii"
There are other issue that you have to take into consideration with both
instruments, With digital image analysis you also have to look at the
questions that CAP just instituted for this. This includes the machine
being run by someone that can do high complexity testing. This means
that you have to have a tech do this machine, not a TA or Lab aid.
There are also issue with consistent calibration of the Image analysis
machines, continued education of the techs, and validating not only
anitbodies but continued validation of the image algorythms.. Do not
listen to vendors, rely on your instinct and validate.
Jesus A Ellin HT/PA ASCP
Department of Pathology/Histology
Yuma Regional Medical Center
2400 South Ave A
Yuma, AZ 85364 - 7170
( Office: (928) 336-1743
( Fax: (928) 336-7319
* Email: jellin <@t> yumaregional.org
______________________________________________________________________
This message is confidential, intended only for the named
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or are not the named recipient(s), please notify the sender
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------------------------------
Message: 20
Date: Tue, 15 Jun 2010 10:01:16 -0600
From: "Liz Chlipala" <liz <@t> premierlab.com>
Subject: RE: [Histonet] Antibody Validation - long response
To: "Rene J Buesa" <rjbuesa <@t> yahoo.com>,
<histonet <@t> pathology.swmed.edu>, <Teri.Hallada <@t> midmichigan.org>
Message-ID:
<EE33BE5C905A3046A7FF8F58A64C8E4B1012D4 <@t> server.PremierLab.local>
Content-Type: text/plain; charset="iso-8859-1"
Bottom line it's not the vendors responsibility to validate their equipment or antibodies in your lab. Some vendors may help you do this, but ultimately the lab needs to validate the equipment and IHC in their lab. The vendors normally calibrate the equipment prior to shipment and once they set the instrument up in your lab, they should be able to provide you with the documentation that states that they calibrated the instrument. Your instruments need to be calibrated prior to being validated.
As far as your scanner goes some vendors can provide validation, but it's at a cost and that cost is not cheap depending upon what you actually want validated. If you are using the scanner and associated algorithms for analysis then you need to validate that separately. There are several steps required to validate a scanner - 1. you validate the scanner 2. if you are using a database to store your images then that also may need to be validated and 3. if you are using algorithms that provide you with data then those algorithms need to be validated.
For example prior to running a validation protocol on a tissue processor its needs to be calibrated for temperature. All of your major equipment needs to be on a calibration schedule. We calibrate all of our instruments once a year and validation is completed only once unless we change the instrument location or how we use the instrument. Pipettors are calibrated every 6 months. All instruments are validated it may just be a one pager for the basic lab equipment but instruments like the tissue processor, slide staining, IHC stainer and scanner require written protocols some of these are 80 pages in length and go into great detail.
The same goes for your antibodies. Antibodies are validated initially with 25 tissue samples (10 strongly positive tissues, 10 moderate to weakly positive tissues and 5 tissues that have no reactivity) This type of validation is required for routine antibodies, prognostic markers such as Her-2, ER and PR require additional tissue samples. New lots require 3 tissue samples one strongly positive on moderate to weakly positive and one negative. If you change the antibody source or detection system or retrieval it needs to be validated again - This information comes from the paper Standarization of Immunohistochemistry from CAP its available on line - I have a copy if you need it. There are also new guidelines for ER/PR and a new article on validation of ER/PR in the June issue of Archives of pathology from CAP.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Tuesday, June 15, 2010 8:50 AM
To: histonet <@t> pathology.swmed.edu; Teri.Hallada <@t> midmichigan.org
Subject: Re: [Histonet] Antibody Validation
Teri:
You are right about the validations you propose although I am not surprised that your vendor does not think it is necessary. They are in the business of selling and you are in the business of assuring the high quality of your work to obtaining the most accurate work for patients' sake.
There is where the difference resides. Ignore your vendor and keep validating your protocols.
René J.
--- On Tue, 6/15/10, Teri.Hallada <@t> midmichigan.org <Teri.Hallada <@t> midmichigan.org> wrote:
From: Teri.Hallada <@t> midmichigan.org <Teri.Hallada <@t> midmichigan.org>
Subject: [Histonet] Antibody Validation
To: histonet <@t> pathology.swmed.edu
Date: Tuesday, June 15, 2010, 7:55 AM
I am being questioned by our vendor as to why we need to validate our
automated immunostainer and image analysis instrument. They would like
documentation pertaining to the requirement of validation and the number
of specimens utilized for validation. I am requesting that each
antibody be validated on the instrument against a previously validated
instrument. Additionally, I am requesting that each new lot of antibody
be validated upon receipt against previously ran specimens. This would
also apply to the image analysis antibodies. (Her2 has been validated by
FISH.) The vendor has apparently polled users in the area and this is
not a standard protocol, therefore the request for documentation.
I think it is pretty clearly stated by CAP in the Quality Management In
Anatomic Pathology. Any other suggestions?
Teresa Hallada BS, MT/CT (ASCP)
Pathology Lead
MidMichigan Health - Gratiot
teri.hallada <@t> midmichigan.org
989.463.1101 ext 3423
___________________________________
Please note that this email message and any attachments may contain privileged and confidential information that is protected against use or disclosure under federal and state law. The information is intended only for the personal and confidential use of the intended recipient. If the reader of this message is not the intended recipient or the employee or agent responsible for delivering it to the intended recipient, you are hereby notified that you have received this information in error and that any review, dissemination, distribution, copying or action taken in reliance on the contents of this communication is strictly prohibited. If you have received this email in error, please advise by immediate reply.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
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End of Histonet Digest, Vol 79, Issue 15
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