[Histonet] RE: Histonet Digest, Vol 79, Issue 13

[Barone, Carol]

We use a modified Gomori's one step....with excellent results on frozen
muscle. You can find the protocol in Muscle Pathology and
Histochemistry, Harvey Sarnat. It is an excellent stain...and if you
have a limited amount of tissue, it gives you the most information on a
single slide....(and if the tissue was quickly... and properly
frozen....will include fiber typing). 

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Subject: Histonet Digest, Vol 79, Issue 13

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Today's Topics:

   1. Re: Can you do Masson's trichrome on frozen sections
      (Robert Richmond)
   2. Ken Marissael is out of the office (Ken_Marissael <@t> vwr.com)
   3. Jaclyn Bhamornsiri is out of the office
      (Jaclyn_Bhamornsiri <@t> vwr.com)


----------------------------------------------------------------------

Message: 1
Date: Sat, 12 Jun 2010 13:59:58 -0400
From: Robert Richmond <rsrichmond <@t> gmail.com>
Subject: [Histonet] Re: Can you do Masson's trichrome on frozen
	sections
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTinZ1bqagomkhGjWs3Lwh1CqNVtjsEhKKzH7u3Oe <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

The Engel-Cunningham variant of the Gomori trichrome stain was developed
for skeletal muscle frozen sections, and is a fairly simple one-step
trichrome stain once you get it working. I've never used it for any
other tissue. I think it's commercially available in kit form.

Bob Richmond
Samurai Pathologist
Knoxville TN



------------------------------

Message: 2
Date: Sat, 12 Jun 2010 14:01:13 -0400
From: Ken_Marissael <@t> vwr.com
Subject: [Histonet] Ken Marissael is out of the office
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	
<OF94ACC4C9.D004A782-ON85257740.0062FD0B-85257740.0062FD0B <@t> vwr.com>
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting 06/12/2010 and will not return
until 06/13/2010





------------------------------

Message: 3
Date: Sat, 12 Jun 2010 14:01:14 -0400
From: Jaclyn_Bhamornsiri <@t> vwr.com
Subject: [Histonet] Jaclyn Bhamornsiri is out of the office
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	
<OF0E1637B9.DB60F492-ON85257740.0062FD75-85257740.0062FD75 <@t> vwr.com>
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting 06/11/2010 and will not return
until 06/12/2010





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End of Histonet Digest, Vol 79, Issue 13
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