[Histonet] RE: Explanted prosthetic valves

Goodwin, Diana Goodwd2 <@t> LabCorp.com
Wed Jun 9 14:30:21 CDT 2010


Hi, Rich.

I've worked at institutions with both policies.  At one, we had a detailed Explanted Medical Device Policy which was developed in cooperation with the Risk Management Department.  The other left it up to the surgeon as to whether or not to send the devices to pathology.  Ultimately, it is up to Risk Management to ensure compliance with explanted medical device requirements.

Hope this helps.

Diana Goodwin



________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Wednesday, June 09, 2010 10:40 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 79, Issue 8

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Today's Topics:

   1. Re: block disposal (DKBoyd <@t> chs.net)
   2. Away for instrument training. (Ken_Marissael <@t> vwr.com)
   3. RE: water bath information (Cynthia Pyse)
   4. Refrigerator Mold (Jennifer Anderson)
   5. Waterbath Options (Breeden, Sara)
   6. RE: water bath information (Truscott, Tom)
   7. RE: Refrigerator Mold (Sherwood, Margaret )
   8. RE: Refrigerator Mold (Jennifer MacDonald)
   9. Special Stain Procedures (thisisann <@t> aol.com)
  10. RE: Refrigerator Mold (Weems, Joyce)
  11. Histology Jobs (K.C. Carpenter)
  12. RE: Refrigerator Mold (Weems, Joyce)
  13. Re: Block Disposal (Debbie Dreesen)
  14. Re: Leica Paraplast (Debbie Dreesen)
  15. Oncotech (Marc & Sandy Shaeffer)
  16. Re: RE: [Histonet] block disposal (Steven Hacker)
  17. Re: Oncotech (Brandi Higgins)
  18. RE: Special Stain Procedures (Horn, Hazel V)
  19. RNA FISN on frozen tissue samples (John Shelley)
  20. Re: Special Stain Procedures (Rene J Buesa)
  21. Re: Refrigerator Mold (Rene J Buesa)
  22. Explanted prosthetic valves (Richard Cartun)
  23. Image Analysis (Bliven, Laura)


----------------------------------------------------------------------

Message: 1
Date: Tue, 8 Jun 2010 13:58:52 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] block disposal
To: Joyce Cline <Joyce.Cline <@t> wchsys.org>
Cc: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,
        histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
        <OF68D5DEE8.0B39864A-ON8525773C.0062B530-8525773C.0062C5EB <@t> chs.net>
Content-Type: text/plain;       charset="US-ASCII"

 We "red bag" (biohazard) ours and they are incinerated.

Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va.  23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net





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------------------------------

Message: 2
Date: Tue, 8 Jun 2010 14:50:08 -0400
From: Ken_Marissael <@t> vwr.com
Subject: [Histonet] Away for instrument training.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <OF4E4D5D4E.4729CE16-ON8525773C.006777CC-8525773C.006777CB <@t> vwr.com>
Content-Type: text/plain; charset=US-ASCII


I will be out of the office starting  06/08/2010 and will not return until
06/14/2010.

I will be out of the field beginning June 8th and will return on June 14th.
Please call Customer Service at 1-800-932-5000 for any of your immediate
needs. I will have very limited access to e-mail and v-mail, but will try
to respond as quickly as I can.




------------------------------

Message: 3
Date: Tue, 8 Jun 2010 15:02:08 -0400
From: "Cynthia Pyse" <cpyse <@t> x-celllab.com>
Subject: RE: [Histonet] water bath information
To: "'Cheryl'" <tkngflght <@t> yahoo.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000901cb073d$181d3d40$4857b7c0$@com>
Content-Type: text/plain;       charset="iso-8859-1"

I am also looking for the same type of waterbaths and would appreciate any
information.
Thanks
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, June 08, 2010 12:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] water bath information

Hi Guys-
?
We need new waterbaths and I'm looking for a specific type.? It's blue with
a square glass baking dish inset on the top.? We don't care if they're blue
but the small footprint with the glass insert is the key--
?
Vendors and refurb dealer responses welcome-
?
Thanks!
?
Cheryl Kerry, HT(ASCP)
Houston
kerryc <@t> labcorp.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet






------------------------------

Message: 4
Date: Tue, 8 Jun 2010 12:34:43 -0700
From: Jennifer Anderson <janderson <@t> halozyme.com>
Subject: [Histonet] Refrigerator Mold
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <5F7CC9B788911848A79BC83453A3D65304116DEF5D <@t> Tomlinson.hti.com>
Content-Type: text/plain; charset="us-ascii"

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks of folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage.
I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>



________________________________
The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email.


------------------------------

Message: 5
Date: Tue, 8 Jun 2010 13:34:13 -0600
From: "Breeden, Sara" <sbreeden <@t> nmda.nmsu.edu>
Subject: [Histonet] Waterbath Options
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <4D14F0FC9316DD41972D5F03C070908B02E47148 <@t> nmdamailsvr.nmda.ad.nmsu.edu>

Content-Type: text/plain;       charset="us-ascii"

Okay, time for my two cents' worth.  Water baths - I don't like the
glass-dish-insert kind because water can get beneath the glass dish and
can temp fluctuations. And if the glass insert breaks, it might be
difficult to find a replacement (Pyrex?).  I like the good ol' fashioned
round or rectangular black-lined fill-it-with-water-and-turn-it-on kind.
They hold temp much better and because they are lined with something
like black Teflon (or whatever), they clean like a dream.  I found that
I really do not like the romantic under-tissue lighting.  It's all about
preferences and this is mine...not that you asked,  but that never
stopped me before, did it?  Happy Tuesday!



Sally Breeden, HT(ASCP)

Veterinary Diagnostic Services

New Mexico Department of Agriculture

700 Camino de Salud NE

Albuquerque, NM  87108

505-841-2576





------------------------------

Message: 6
Date: Tue, 8 Jun 2010 12:39:46 -0700
From: "Truscott, Tom" <ttruscot <@t> vetmed.wsu.edu>
Subject: RE: [Histonet] water bath information
To: Cynthia Pyse <cpyse <@t> x-celllab.com>, 'Cheryl'
        <tkngflght <@t> yahoo.com>,  "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <44F1D6D7EB8CC84F92859EE5C4E6ECB401134BAFFD49 <@t> CVMMBX.vetmed.wsu.edu>
Content-Type: text/plain; charset="iso-8859-1"

Hi, The old TBS's are blue, and the new ones are white. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Tuesday, June 08, 2010 12:02 PM
To: 'Cheryl'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] water bath information

I am also looking for the same type of waterbaths and would appreciate any
information.
Thanks
Cindy Pyse, CLT, HT (ASCP)
Histology Supervisor
X-Cell Laboratories
e-mail cpyse <@t> x-celllab.com




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl
Sent: Tuesday, June 08, 2010 12:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] water bath information

Hi Guys-
?
We need new waterbaths and I'm looking for a specific type.? It's blue with
a square glass baking dish inset on the top.? We don't care if they're blue
but the small footprint with the glass insert is the key--
?
Vendors and refurb dealer responses welcome-
?
Thanks!
?
Cheryl Kerry, HT(ASCP)
Houston
kerryc <@t> labcorp.com
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 7
Date: Tue, 8 Jun 2010 16:08:31 -0400
From: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Subject: RE: [Histonet] Refrigerator Mold
To: "Jennifer Anderson" <janderson <@t> halozyme.com>,
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <073AE2BEA1C2BA4A8837AB6C4B943D9703E243C5 <@t> PHSXMB30.partners.org>
Content-Type: text/plain; charset="us-ascii"

By folders, I assume you mean the cardboard ones.  Why not store the slides in
something else, i.e. plastic boxes?



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Tuesday, June 08, 2010 3:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks of
folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that
seems to have worked, but I'm not sure if that is detrimental to slide and
reagent storage.
I have purchased some strong disinfectants also, but I don't know if those
vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>



________________________________
The information transmitted in this email is confidential and is intended only
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to any person other than the intended recipient(s) is not intended in any way to
waive confidentiality or any applicable privilege. Any review, retransmission,
dissemination or other use of, or taking of any action in reliance upon, this
information by individuals or entities other than the intended recipient is
prohibited by Halozyme and may be in violation of applicable laws. If you
received this in error, please contact the sender and delete/destroy this email.
_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it is
addressed. If you believe this e-mail was sent to you in error and the e-mail
contains patient information, please contact the Partners Compliance HelpLine at
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------------------------------

Message: 8
Date: Tue, 8 Jun 2010 13:42:22 -0700
From: Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
Subject: RE: [Histonet] Refrigerator Mold
To: "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Cc: histonet <@t> lists.utsouthwestern.edu,
        histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
        <OFA24D66A0.C68F3CFD-ON8825773C.0071B0E8-8825773C.0071C1AE <@t> mtsac.edu>
Content-Type: text/plain; charset="US-ASCII"

Bleach can cause corrosion of the metal parts.




"Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/08/2010 01:36 PM

To
"Jennifer Anderson" <janderson <@t> halozyme.com>,
<histonet <@t> lists.utsouthwestern.edu>
cc

Subject
RE: [Histonet] Refrigerator Mold






By folders, I assume you mean the cardboard ones.  Why not store the
slides in
something else, i.e. plastic boxes?



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer
Anderson
Sent: Tuesday, June 08, 2010 3:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have
stacks of
folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and
that
seems to have worked, but I'm not sure if that is detrimental to slide and
reagent storage.
I have purchased some strong disinfectants also, but I don't know if those
vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>



________________________________
The information transmitted in this email is confidential and is intended
only
for the person(s) or entity to which it is addressed. Delivery of this
message
to any person other than the intended recipient(s) is not intended in any
way to
waive confidentiality or any applicable privilege. Any review,
retransmission,
dissemination or other use of, or taking of any action in reliance upon,
this
information by individuals or entities other than the intended recipient
is
prohibited by Halozyme and may be in violation of applicable laws. If you
received this in error, please contact the sender and delete/destroy this
email.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


The information in this e-mail is intended only for the person to whom it
is
addressed. If you believe this e-mail was sent to you in error and the
e-mail
contains patient information, please contact the Partners Compliance
HelpLine at
http://www.partners.org/complianceline . If the e-mail was sent to you in
error
but does not contain patient information, please contact the sender and
properly
dispose of the e-mail.


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 9
Date: Tue, 08 Jun 2010 16:43:26 -0400
From: thisisann <@t> aol.com
Subject: [Histonet] Special Stain Procedures
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <8CCD56260E1D992-1218-2604 <@t> webmail-m094.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"


Does anyone have the following procedures they can share with me:
- Rhodamine (for copper)
- Victoria Blue
Thank you,
Ann





------------------------------

Message: 10
Date: Tue, 8 Jun 2010 17:41:15 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Refrigerator Mold
To: Jennifer Anderson <janderson <@t> halozyme.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C205 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

Scrape off the mold and make us all some control blocks!!
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson [janderson <@t> halozyme.com]
Sent: Tuesday, June 08, 2010 3:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks of folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage.
I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>



________________________________
The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential.  Any unauthorized review, use,
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not the intended recipient, please delete this message, and
reply to the sender regarding the error in a separate email.




------------------------------

Message: 11
Date: 8 Jun 2010 17:42:06 -0400
From: "K.C. Carpenter" <kc <@t> ka-recruiting.com>
Subject: [Histonet] Histology Jobs
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <1116999842.1276033325915.JavaMail.cfservice <@t> sl4app2>
Content-Type: text/plain; charset="utf-8"


Hello Everyone,



I am one of the owners of a healthcare recruiting firm that specializes in placing Lab Professionals and I wanted to see if you'd be interested in learning about potential career advancing job opportunities? We are completely free of charge to candidates and can be a great resource for you in helping you to find your next job. Our clients offer competitive compensation packages and typically assist with relocation expenses. I am currently working on some great job opportunities in various areas across the country.


One position in particular you may be interested in is a 1st shift Histotechnologist job located outside of Indianapolis. This opportunity is with a 250 bed hospital that is consistently ranked as being one of the top 10 employers in Indiana.  My client is offering an above average compensation package, excellent benefits and relocation assistance when necessary. Qualified candidates must be HT or HTL (ASCP) certified with prior experience working in a clinical lab setting.  Please let me know if you're be interested in hearing more about this position and learning if it might be the next step in your career.



Below is a list of some of the opportunities we're currently working on. If you do not see an opening in a location in which you live or would like to live, please send me an email me a copy of your resume and let me know where you would be interested in a job. I will then tailor a search for you that is completely confidential.





IN - Columbus - Histotechnologist (1st Shift) *****New Opening*****


GA - Histology Supervisor


NY- Upstate- Histotech (1st Shift)


NY- New York City - Histology Supervisor


NY - New York City - Histotech (3rd shift)


NY - New York City - Grossing Technologist *****New Opening*****




NV -Las Vegas - Histotech (3rd shift)


NV Las Vegas - Histology Supervisor (3rd shift)


CA - Southern - Histotech


GA - Atlanta - Histotech


MA - Histotech




MA - IHC Tech




























If you're interested in learning more about these opportunities or opportunities in a certain geographic location please reply with an updated resume and let me know when a good time to reach you is. If this is not the right time for you to make a career change then please let me know if you can refer someone who is.  We offer a generous referral bonus for anyone you refer to us that we place into any position across the country.




To learn more about us please visit our website at www.ka-recruiting.com.












Sincerely












KC Carpenter



K.A. Recruiting, Inc.


10 Post Office Square, 8th Floor South


Boston, MA 02109


P: (617) 692-2949


F: (617) 507-8009



kc <@t> ka-recruiting.com



www.ka-recruiting.com










------------------------------

Message: 12
Date: Tue, 8 Jun 2010 17:42:01 -0400
From: "Weems, Joyce" <JWeems <@t> sjha.org>
Subject: [Histonet] RE: Refrigerator Mold
To: Jennifer Anderson <janderson <@t> halozyme.com>,
        "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <92AD9B20A6C38C4587A9FEBE3A30E164015DD6C206 <@t> CHEXCMS10.one.ads.che.org>
Content-Type: text/plain; charset="us-ascii"

oops = didn't intend to hit send..

But perhaps try dessicant or baking soda, which would reduce the moisture....
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson [janderson <@t> halozyme.com]
Sent: Tuesday, June 08, 2010 3:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Refrigerator Mold

Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.  We have stacks of folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage.
I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>



________________________________
The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Confidentiality Notice:
This e-mail, including any attachments is the
property of Catholic Health East and is intended
for the sole use of the intended recipient(s).
It may contain information that is privileged and
confidential.  Any unauthorized review, use,
disclosure, or distribution is prohibited. If you are
not the intended recipient, please delete this message, and
reply to the sender regarding the error in a separate email.




------------------------------

Message: 13
Date: Tue, 8 Jun 2010 14:56:50 -0700 (PDT)
From: Debbie Dreesen <ddreesen <@t> sbcglobal.net>
Subject: [Histonet] Re: Block Disposal
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <49117.29704.qm <@t> web81002.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Joyce,
We disposed of of tissues and their containers after 6 weeks on the shelf. Outdated blocks were placed in the same totes used for the tissues and picked up by an outside company for incineration.

Debbie Dreesen, HT(ASCP)



Message: 9
Date: Tue, 8 Jun 2010 10:27:55 -0400
From: Joyce Cline <Joyce.Cline <@t> wchsys.org>
Subject: [Histonet] block disposal
To: "histonet <@t> lists.utsouthwestern.edu"
??? <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
??? <FE2197D76184F4408CFEBD95C28282685BF36C85A9 <@t> WCHSXCHCM.wchsys.org>
Content-Type: text/plain; charset="iso-8859-1"

How does everyone handle disposing of blocks that are more than 10 years old? Are they incinerated, hauled away by a waste management company or just thrown in the trash.

In Maryland I was told they can't be thrown in the regular trash by a state waste official.

Joyce Cline, Technical Specialist
Hagerstown Medical Laboratory
301-665-4980
fax 301-665-4941
joyce.cline <@t> wchsys.orgmailto:joyce.cline <@t> wchsys.org



------------------------------

Message: 14
Date: Tue, 8 Jun 2010 15:49:20 -0700 (PDT)
From: Debbie Dreesen <ddreesen <@t> sbcglobal.net>
Subject: [Histonet] Re: Leica Paraplast
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <981456.12795.qm <@t> web81004.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hi Becky,
There were several posts between March 23-31 addressing the quality of the Paraplast if you want to check them out.?We tried trouble-shooting everything we could think of when we noticed a change in the paraffin.?The texture was very different?and it was so?sticky?our sections stuck to the blade no matter how we tried to fix the problem.?It was gritty and caused nicks in the blade after just a few sections. We tried several different blades to make sure it wasn't a problem there or a bad batch.?Different brands as well as both low?and high profile. We?tried the different blades and settings because we had just replaced all our microtomes?and thought maybe we just needed to make some adjustments but we experienced the same problem with each?blade at every setting we tried?and concluded the paraffin was the problem.?The issues?were resolved after we switched back to ordering the EM-400 from Cardinal.


Debbie Dreesen, HT(ASCP)



Message: 3
Date: Thu, 3 Jun 2010 15:20:37 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Paraplast
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AE1A09212F464CE6A03EF4117C4137AB <@t> CHURCH>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
??? reply-type=original

What paraffin is everyone using. We have been a Paraplast user made by
McCormick.? We have noticed black to gray small dusty particles in the
embedding well. I have tried a couple others, but the ribbons compress.? I
have tried the a different angle at 3 with no difference.? I would
appreciate some help from all of you.
Thanks
Becky



------------------------------

Message: 15
Date: Tue, 8 Jun 2010 19:19:43 -0400 (EDT)
From: Marc & Sandy Shaeffer <mshaeffer <@t> cox.net>
Subject: [Histonet] Oncotech
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Cc: arsenn <@t> hsh.org, kbernhisel <@t> precisiontherapeutics.com
Message-ID: <6218769.30833.1276039183117.JavaMail.mshaeffer <@t> 127.0.0.1>
Content-Type: text/plain; charset=UTF-8; format=flowed; delsp=no

Amy Senn asked:

Now that Oncotech is out of business, who is everyone sending their
extreme resistance assays to?

We were just briefed today by a company called Precision Therapeutics
out of Pittsburgh PA that they perform Chemo sensitivity and resistance
tests and the test is called ChemoFx.  Their website is
www.precisiontherapeutics.com.  I have no affiliation with this company.

Precision Therapeutics supplies the shipping container and fluid,
usually overnight delivery with Saturday deliveries allowed.

Marc Shaeffer
Tucson Medical Center
Arizona



------------------------------

Message: 16
Date: Tue, 08 Jun 2010 18:54:11 -0500 (CDT)
From: Steven Hacker <stevenhacker <@t> verizon.net>
Subject: Re: RE: [Histonet] block disposal
To: jstaruk <@t> masshistology.com
Cc: histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <1232490361.717331.1276041251677.JavaMail.root <@t> vms246.mailsrvcs.net>
Content-Type: text/plain; charset="UTF-8"


   Hey, Joyce...

   Contact  some  of  your  vendors...Many  of  them  would  love  to get
   addition   companies  will d   compensate/donate  to  th   difference in future assays.



   Regards,

   Steven Hacker



   Ps...Yes,  I  work  for Biocare Medical and we're always interested in
   bl   prefer  to kn   and   if   they   were  po   information will ever be accepted.<   Jun 8, 2010 01:38:11 PM, jstaruk <@t> masshistology.com wrote:
   <
     We  are  also  s     returning all
     blocks back t     _______________________
     James E. Staruk HT(ASCP)www.masshistology.com
     www.nehorselabs.com
     -----Orig     From: histonet-bounces <@t> lists.utsouthwestern.edu
     [ma     Sherwood,
     M     Sent: Tuesday, June 08, 2010 11:36 AM
     To: Joyce Cline; histo     Subject: RE: [Histonet] block disposal
     <     We  are  a  research laboratory. Our policy, based on communication
     via     Histonet,  is  to keep 2 years worth of paraffin blocks in the lab.
     B     greater than 2 years old up to 10 years old, we send to off-site s     torage.
     Blocks  greater  than 10 years old are destroyed by the storage co     mpany since
     they have them.
     When  we first set up this system last     of blocks
     and the majority were put in our      incinerated.
     Peggy
          From: histonet-bounces <@t> lists.utsouthwestern.e     [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]   On  Behalf  Of
     Joyce     Sent: Tuesday, June 08, 2010 10:28 AM
     To: histonet <@t> lists.utsou     Subject: [Histonet] block disposal
     How  does  everyon     10 years
     old?  Are  they  i     company or just
     thrown in      In  Maryland I was told they can't be thrown in the regula     by a state
     waste official.
     Joyce Cline, Technical Special     Hagerstown Medical Laboratory
     301-665-4980
     fax 301-665-4941 jo     ________________________________
     *****   CONFIDENT     confidential
     information and i     are not
     the  named  addre     copy this
     e-mail.  Please       have received
     this e-mail     ___________________     Histonet mailing list
     Histonet <@t> lists.uts     http://lists.utsouthwestern.edu/mailman/listinfo/histone     The  information in this e-mail is intended only for the person     whom it is
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     http://lists.utsouthw


------------------------------

Message: 17
Date: Tue, 8 Jun 2010 19:59:31 -0400
From: Brandi Higgins <brandihiggins <@t> gmail.com>
Subject: Re: [Histonet] Oncotech
To: "Senn, Amy R" <arsenn <@t> hsh.org>
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
        <AANLkTim5SS5SRHDBos3i7TCnretk-IqV4seGI1KgHily <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Hello,

Many of our surgeons who previously requested we send specimens to Oncotech
switched to Precision Therapeutics about a year or so ago (although some
still used Oncotech at times).  We have used Precision with no problems.
They even supplied us with a refrigerator to store the specimen collection
kits which need to be refrigerated, and our reps have been very helpful
overall.  Hope this helps.

Brandi Higgins, HT(ASCP)
On Mon, Jun 7, 2010 at 9:27 AM, Senn, Amy R <arsenn <@t> hsh.org> wrote:

> Hello Histonet,
>
>
>
>
>
> Now that Oncotech is out of business, who is everyone sending their
> extreme resistance assays to?
>
>
>
>
>
> Thanks,
>
> Amy, HT
>
> Camp Hill, PA
>
>
>
>
>
>
>
>
>
>
>
> Attention:  This Message is intended only for the use of the individual or
> entity to which it is addressed, and may contain information that is
> privileged, confidential and exempt from disclosure under applicable law. If
> the reader of this message is not the intended recipient, you are hereby
> notified that any dissemination or copying of this message or the taking of
> any action in reliance on the contents of this message is strictly
> prohibited. If you have received this message in error, please notify us
> immediately and destroy the original message. Thank you.
> _______________________________________________
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> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


------------------------------

Message: 18
Date: Wed, 9 Jun 2010 07:07:39 -0500
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] Special Stain Procedures
To: <thisisann <@t> aol.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <9AE8AA9E1F644B4AA6C155FB6FD51C6317D838AA <@t> EMAIL.archildrens.org>
Content-Type: text/plain;       charset="US-ASCII"

For copper we use the kit from American Master Tech.  It works really
well.

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3155

visit us on the web at:    www.archildrens.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
thisisann <@t> aol.com
Sent: Tuesday, June 08, 2010 3:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Special Stain Procedures


Does anyone have the following procedures they can share with me:
- Rhodamine (for copper)
- Victoria Blue
Thank you,
Ann



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------------------------------

Message: 19
Date: Wed, 9 Jun 2010 08:21:50 -0400
From: John Shelley <jshelley <@t> burnham.org>
Subject: [Histonet] RNA FISN on frozen tissue samples
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
        <CEDED70E7CD1FD418B1525ABD333554F7BDD444052 <@t> LN-MAIL07.ln.burnham.org>
Content-Type: text/plain; charset="us-ascii"

Hi Histonetters,

I have a huge favor to ask, I have been asked to start up work on frozen tissue samples to perform RNA FISH. I am looking for some individuals how are doing this or attempting to do this and can help shed some light on this undertaking. We successfully were able to get this protocol to work on cultures cells form a few different lines but know that the transition may be much more difficult in human tissue samples. Again any help or advise anyone can give would be greatly appreciated. If you want to contact me offline that would be fine. Thanks in advance for any help.

John


------------------------------

Message: 20
Date: Wed, 9 Jun 2010 06:01:44 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Special Stain Procedures
To: histonet <@t> lists.utsouthwestern.edu, thisisann <@t> aol.com
Message-ID: <929438.23665.qm <@t> web65704.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Neither procedure is reliable. Use Tim's method (sulfur dioxide).
Ren? J.

--- On Tue, 6/8/10, thisisann <@t> aol.com <thisisann <@t> aol.com> wrote:


From: thisisann <@t> aol.com <thisisann <@t> aol.com>
Subject: [Histonet] Special Stain Procedures
To: histonet <@t> lists.utsouthwestern.edu
Date: Tuesday, June 8, 2010, 4:43 PM



Does anyone have the following procedures they can share with me:
- Rhodamine (for copper)
- Victoria Blue
Thank you,
Ann



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 21
Date: Wed, 9 Jun 2010 06:08:21 -0700 (PDT)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] Refrigerator Mold
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,    Jennifer Anderson
        <janderson <@t> halozyme.com>
Message-ID: <316872.16387.qm <@t> web65706.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

You should never use an open container with bleach in any place where you keep slides, because bleach will affect the antigens in the sections and affect histochemical reactions as well.
Carboard folders will catch fungus spores outside the refrigerator that eventually will develop into fungi, as in your case. Carboard folders are not a good option to store slides in the humid environment of a refrigerator.
You should put your slides in plastic boxes, either in large ones (100 slides each) or small ones (25 slides).
You will be able alto to store more slides in less space.
Ren? J.
?
?

--- On Tue, 6/8/10, Jennifer Anderson <janderson <@t> halozyme.com> wrote:


From: Jennifer Anderson <janderson <@t> halozyme.com>
Subject: [Histonet] Refrigerator Mold
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Tuesday, June 8, 2010, 3:34 PM


Hello and Greetings.

I am looking for a solution to our refrigerator mold problem.? We have stacks of folders in our refrigerators, and they get moldy with time.
I have placed a small open container of bleach on the bottom shelf, and that seems to have worked, but I'm not sure if that is detrimental to slide and reagent storage.
I have purchased some strong disinfectants also, but I don't know if those vapors will harm slides or reagents.

Thanks so much for all of your continued insight and advice!

Jennifer M. Anderson, Scientist
Halozyme Therapeutics, Inc.
11388 Sorrento Valley Road
San Diego, CA 92121
858-704-8333 (office)
janderson <@t> halozyme.com<mailto:janderson <@t> halozyme.com>



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The information transmitted in this email is confidential and is intended only for the person(s) or entity to which it is addressed. Delivery of this message to any person other than the intended recipient(s) is not intended in any way to waive confidentiality or any applicable privilege. Any review, retransmission, dissemination or other use of, or taking of any action in reliance upon, this information by individuals or entities other than the intended recipient is prohibited by Halozyme and may be in violation of applicable laws. If you received this in error, please contact the sender and delete/destroy this email.
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------------------------------

Message: 22
Date: Wed, 09 Jun 2010 09:41:50 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: [Histonet] Explanted prosthetic valves
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4C0F61DE.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII

For those of you who work in hospitals:

What is your policy on explanted prosthetic valves?  Discard or send to pathology?  Thanks.

Richard

Richard W. Cartun, Ph.D.
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax





------------------------------

Message: 23
Date: Wed, 09 Jun 2010 08:47:09 -0500
From: "Bliven, Laura" <bliven.laura <@t> marshfieldclinic.org>
Subject: [Histonet] Image Analysis
To: "histonet <@t> lists.utsouthwestern.edu"
        <histonet <@t> lists.utsouthwestern.edu>,    "ihcrg <@t> googlegroups.com"
        <ihcrg <@t> googlegroups.com>
Message-ID: <201006091347.o59DlBca021717 <@t> spamfilt>
Content-Type: text/plain; charset="iso-8859-1"

What software systems is everyone in clinical settings using for image analysis of markers such as p53, MIB1, Estrogen, Progesterone, HER2 IHC, possibly and HER2 FISH for starters? What characteristics do you like and not like about your system?
Thanks for time and feedback. Feedback is so valuable in everyone's job, no matter where you work.
Laura

Laura Bliven, AAS, HT(ASCP), QIHC
Histology Lab/IHC
Marshfield Laboratories
1000 N. Oak Ave.
Marshfield, WI 54449



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