[Histonet] H&E Stainer Validations

[Rene J Buesa]

Albert:
In histology there are only very few standards. That is why CAP has a check list when inspecting histolabs.
If you are doing a "validation", get as many different tissue sections as you can. Stain them "as usual" (namely using the method you are trying to substitute).
Stain identical sections with your method or instruyment. Label them just by numbers, and give them to the pathologist for a blind evaluation.
They should answer just one questions: is either section within each pair better than the other (telling which one) or are they equally useful for diagnosis.
The results analyze with Chi a square test where the "theoretical" frequency is P=0.50.
As "simple" as that.
René J. 

--- On Fri, 6/4/10, Santiago, Albert <Albert.Santiago <@t> uphs.upenn.edu> wrote:


From: Santiago, Albert <Albert.Santiago <@t> uphs.upenn.edu>
Subject: [Histonet] H&E Stainer Validations
To: histonet <@t> lists.utsouthwestern.edu
Date: Friday, June 4, 2010, 11:30 AM


Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was
wondering if anyone knows if there's a standard H&E Validation protocol
or if it just varies from lab to lab? Thanks for your help.... :-)

Albert Santiago, HT(ASCP)
Laboratory Manager
Dermatopathology
215-662-6759/6539-office
215-520-3797-BB
215-662-7520-fax
We will be relocating, as of 6/14/2010 
our lab will be located
at 3020 Market St. Ste. 201



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