[Histonet] PARAPLAST QUALITY
Karen Kay
Karen.Kay <@t> albertahealthservices.ca
Fri Jun 4 12:43:05 CDT 2010
Good Morning,
Our Paraplast has also been produced by McCormick and to this point have not seen any issues. However it is my understanding that Leica(Surgipath) has taken over as supplier and producer of the Paraplast (trademark). This information was relayed to us by our Surgipath representative quite a few months ago.
This past week, I received a phone call from a different Lab supplier indicating to me that there have been issues cropping up with the Paraplast from the new manufacturer.
I did do a search on the Histonet and have not found messages to that effect. It would be interesting and proactive to find out if there indeed have been issues cropping up recently with the Paraplast.
Thanks
Karen J Kay, MLT
Supervisor - Histopathology and Cytology Laboratory
Chinook Regional Hospital-South Zone West - Alberta Health Services
Lethbridge, Alberta, Canada
karen.kay <@t> albertahealthservices.ca
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: June 4, 2010 11:23 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 79, Issue 4
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Today's Topics:
1. outside review. (Tench, Bill)
2. Laryngotracheitis (Charles, Roger)
3. Paraplast (Rebecca Johnson)
4. RE: Paraplast (Baez, Janet)
5. Lab Manager Job Opportunity in San Francisco
(Adam.T.Anthony <@t> kp.org)
6. coverslippers (Gale Limron)
7. RE: coverslippers (Horn, Hazel V)
8. INI-1 (Horn, Hazel V)
9. Re: Paraplast (DKBoyd <@t> chs.net)
10. AW: INI-1 (Kappeler, Andreas (PATHOLOGY))
11. RE: INI-1 (McMahon, Loralee A)
12. Unsubscribe. (McCormick, James)
13. H&E Stainer Validations (Santiago, Albert)
14. Re: Unsubscribe. (Emily Sours)
----------------------------------------------------------------------
Message: 1
Date: Thu, 3 Jun 2010 10:49:45 -0700
From: "Tench, Bill" <Bill.Tench <@t> pph.org>
Subject: [Histonet] outside review.
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <2820431BF953BB4DA3E9E1A5882265FD0286317D <@t> MAIL1.pph.local>
Content-Type: text/plain; charset=us-ascii
I thought I sent this yesterday, but it didn't address properly. I guess things are different in my part of the world in terms of the frequency of this activity compared with others. Please see the rest.
The policy/procedure requiring review of outside slides before definitive treatment (including surgery, radiation therapy, or
chemotherapy) in another institution is a fairly common but frequently unenforced policy in many hospitals other than academic institutions.
The policy may reside in the Pathology Dept, Surgery Dept, or more frequently (and safely) as a standard Hospital policy. Typically, enforceability is improved if the policy is a Hospital policy. There is an ever growing body of evidence that this policy benefits the patient, the hospital, the clinician, and the pathologist. Compliance is always an issue, especially outside of academic centers, who, out of a belief that the community pathologists are prone to mistakes that academic centers never make, insist on review before treatment. "Everyone's benefit" is always a nice "carrot," but unfortunately, without a "stick," compliance may be a challenge. For the surgeons, at least, the risk of cancellation of the case if a TIMELY request for outside review has not been made serves as a useful "stick," the application of which should be applied judiciously (and perhaps with a flack jacket).
Radiation and Chemo cases require either: 1) cooperation of these departments or 2) external review of all cases treated in those departments to determine whether or not case review has occurred. The goal is to, as best as possible, confirm the diagnosis as well as other aspects that may have an impact on the appropriate therapy, and to do so without significant delay in the patient's care. Anything that makes the process easier is a good idea. In my system, the responsibility of the treating physician is to fax to the pathology dept a copy of the report when they have decided to proceed with additional treatment (surgery, rads, etc), and the pathology dept does all the work associated with requesting the slides and insuring a timely review. We advise (and continuously train) the outside office staff that they must provide us this information ASAP because some outside institutions are woefully slow in sending the material to us. (For requests to us from other institutions, we typically send the material to the requesting institution within 24 hours if we don't have to prepare recuts. We re-review all material going elsewhere, and we only send original slides if we have multiple representative levels in our file or we have very limited material-like FNA's, otherwise we recut the case).
Dr Bill Tench
Assoc Dir. Lab Services
Chief Cytology
Palomar Medical Center
555 E. Valley Parkway
Escondido, Ca 92025
Voice: 760-739-3037
Fax: 760-739-2604
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Message: 2
Date: Thu, 3 Jun 2010 14:42:07 -0400
From: "Charles, Roger" <rcharles <@t> state.pa.us>
Subject: [Histonet] Laryngotracheitis
To: "Histonet (histonet <@t> lists.utsouthwestern.edu)"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<3809C163DC1DA54AA534B3C7794D07B65A8F32BAB1 <@t> ENHBGMBX01.PA.LCL>
Content-Type: text/plain; charset="us-ascii"
Hello,
Is there someone in the veterinary field doing IHC or FA (ffpe or fresh tissue) for infectious laryngotracheitis? I'm searching for a protocol and an antibody.
Thanks so much
Roger
Roger Charles
Microbiologist II
PA Veterinary Laboratory
717-787-8808
------------------------------
Message: 3
Date: Thu, 3 Jun 2010 15:20:37 -0500
From: "Rebecca Johnson" <raj <@t> bluemarble.net>
Subject: [Histonet] Paraplast
To: "histonet" <Histonet <@t> lists.utsouthwestern.edu>
Message-ID: <AE1A09212F464CE6A03EF4117C4137AB <@t> CHURCH>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
reply-type=original
What paraffin is everyone using. We have been a Paraplast user made by
McCormick. We have noticed black to gray small dusty particles in the
embedding well. I have tried a couple others, but the ribbons compress. I
have tried the a different angle at 3 with no difference. I would
appreciate some help from all of you.
Thanks
Becky
------------------------------
Message: 4
Date: Thu, 3 Jun 2010 13:52:51 -0700
From: "Baez, Janet" <jbaez <@t> interscopepath.com>
Subject: RE: [Histonet] Paraplast
To: "Rebecca Johnson" <raj <@t> bluemarble.net>, "histonet"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9E956D8FEB06C2408B08AC16498325E90F83DF <@t> scopemx1.interscope.com>
Content-Type: text/plain; charset="us-ascii"
We use Richard Allan Scientific Paraffin 3 (item #8335) works great for
embedding and infiltrating.
Janet
Interscope Pathology
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rebecca
Johnson
Sent: Thursday, June 03, 2010 1:21 PM
To: histonet
Subject: [Histonet] Paraplast
What paraffin is everyone using. We have been a Paraplast user made by
McCormick. We have noticed black to gray small dusty particles in the
embedding well. I have tried a couple others, but the ribbons compress.
I
have tried the a different angle at 3 with no difference. I would
appreciate some help from all of you.
Thanks
Becky
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Thu, 3 Jun 2010 16:44:04 -0700
From: Adam.T.Anthony <@t> kp.org
Subject: [Histonet] Lab Manager Job Opportunity in San Francisco
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
<OFA2F30AF4.6B1AE883-ON88257737.007E0603-88257737.00825C0F <@t> kp.org>
Content-Type: text/plain; charset="US-ASCII"
A position is opening up in July as Lab Manager of the Pathology
Department at the Kaiser Permanente San Francisco Medical Center. This is
your chance to be part of an exciting department in a wonderful
organization. Generous compensation package with great benefits await
qualified applicants. Interested candidates should email me for more
details (please include a recent resume).
Regards,
Adam Anthony
Manager, Pathology & Regional Immunohistochemistry
Kaiser Permanente Medical Center
350 St. Joseph's St.
San Francisco, CA 94115
email: adam.t.anthony <@t> kp.org
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Message: 6
Date: Fri, 4 Jun 2010 06:46:18 -0400
From: Gale Limron <GaleL <@t> unionhospital.org>
Subject: [Histonet] coverslippers
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<D7B0000361AAAF4A8895E5918634850A032A4FD785 <@t> uhexg-3.unionhospital.org>
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We are currently evaluating coverslippers and I'd appreciate any info/opinions on the following:
Hacker HCM 6000
TBS SHUR/Mount
Thermo Scientific (Shandon) ClearVue
Tissue-Tek Glas g2
Leica CV5030
We will be looking at stainers within a couple of years so this is something we need to consider also.......
Thanks,
Gale
Gale Limron
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext. 2562
This e-mail is intended only for the person or entity to which it is addressed and may contain information that is privileged, confidential or otherwise protected from disclosure. Dissemination, distribution or copying of this e-mail or the information herein by anyone other than the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, is prohibited. If you received this message in error, please delete without copying and kindly e-mail a reply to inform us of the mistake in delivery.
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Message: 7
Date: Fri, 4 Jun 2010 06:53:10 -0500
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: RE: [Histonet] coverslippers
To: "Gale Limron" <GaleL <@t> unionhospital.org>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C6317D83896 <@t> EMAIL.archildrens.org>
Content-Type: text/plain; charset="US-ASCII"
We love our CV5030 glass coverslipper. It integrates with their stainer
for a seamless flow. It's so nice to put the slides on to stain and
have them coverslipped when they come off.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gale
Limron
Sent: Friday, June 04, 2010 5:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] coverslippers
We are currently evaluating coverslippers and I'd appreciate any
info/opinions on the following:
Hacker HCM 6000
TBS SHUR/Mount
Thermo Scientific (Shandon) ClearVue
Tissue-Tek Glas g2
Leica CV5030
We will be looking at stainers within a couple of years so this is
something we need to consider also.......
Thanks,
Gale
Gale Limron
Histology Supervisor
Union Hospital
659 Boulevard
Dover, Ohio 44622
330-343-3311 ext. 2562
This e-mail is intended only for the person or entity to which it is
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Message: 8
Date: Fri, 4 Jun 2010 06:55:39 -0500
From: "Horn, Hazel V" <HornHV <@t> archildrens.org>
Subject: [Histonet] INI-1
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9AE8AA9E1F644B4AA6C155FB6FD51C6317D83897 <@t> EMAIL.archildrens.org>
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We are having problems with this immunostain. If your lab is
successfully using this antibody will you please help me out?
Where do you purchase your antibody? Is it RTU? What type of antigen
retrieval? Incubation time?
Thanks.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
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The information contained in this message may be privileged and confidential
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Message: 9
Date: Fri, 4 Jun 2010 09:08:43 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] Paraplast
To: "Rebecca Johnson" <raj <@t> bluemarble.net>
Cc: histonet <Histonet <@t> lists.utsouthwestern.edu>,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFE6529BB7.61929396-ON85257738.00482152-85257738.004835C8 <@t> chs.net>
Content-Type: text/plain; charset="US-ASCII"
We use Richard Allen, Type 6 for both impregnation and embedding.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
"Rebecca Johnson" <raj <@t> bluemarble.net>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
06/03/2010 04:42 PM
To
"histonet" <Histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] Paraplast
What paraffin is everyone using. We have been a Paraplast user made by
McCormick. We have noticed black to gray small dusty particles in the
embedding well. I have tried a couple others, but the ribbons compress. I
have tried the a different angle at 3 with no difference. I would
appreciate some help from all of you.
Thanks
Becky
_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 10
Date: Fri, 4 Jun 2010 15:28:00 +0200
From: "Kappeler, Andreas (PATHOLOGY)"
<andreas.kappeler <@t> pathology.unibe.ch>
Subject: [Histonet] AW: INI-1
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4EAFACC375BF974C8A57D33444CEE288049AEFDD64 <@t> AAI-MBX3.campus.unibe.ch>
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Hi Hazel
We use INI-1, clone 25/BAF47, BD Transduction 612110. Working conc. is 2.5 mg Ig/l (1:100), HIER at high pH with a somewhat strange buffer (800 mM urea, 100 mM Tris, pH 9.5), but you may try EDTA-based HIER solutions, pH 8.8-9.5. Incubation at RT, 60 min. Visualization with polymer-based HRP system/DAB. Hope this helps.
Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland
P.S. when asking about advice on antibodies, try to be as specific as possible: clone, Ig-conc and/or dilution, retrieval protocols tried, visualization systems tried. Helps o narrow down possilbe problems.
________________________________________
Von: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] im Auftrag von Horn, Hazel V [HornHV <@t> archildrens.org]
Gesendet: Freitag, 4. Juni 2010 13:55
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] INI-1
We are having problems with this immunostain. If your lab is
successfully using this antibody will you please help me out?
Where do you purchase your antibody? Is it RTU? What type of antigen
retrieval? Incubation time?
Thanks.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
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------------------------------
Message: 11
Date: Fri, 4 Jun 2010 09:36:16 -0400
From: "McMahon, Loralee A" <Loralee_Mcmahon <@t> URMC.Rochester.edu>
Subject: [Histonet] RE: INI-1
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<C27AA2A01CEF31469813089E226F582E025DCFA5B6 <@t> URMCMS7.urmc-sh.rochester.edu>
Content-Type: text/plain; charset="us-ascii"
We have been using an INI-1 from Cell Marque. With a 1/50 dilution and a low pH heat retrieval. Although I accidently had a slide go through the higher pH heat retrieval and I didn't see a big difference in the staining.
I use the Dako system so it was a 30 minute incubation with a linker added.
Hope that helps.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V [HornHV <@t> archildrens.org]
Sent: Friday, June 04, 2010 7:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] INI-1
We are having problems with this immunostain. If your lab is
successfully using this antibody will you please help me out?
Where do you purchase your antibody? Is it RTU? What type of antigen
retrieval? Incubation time?
Thanks.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's Way Slot 820
Little Rock, AR 72202
phone 501.364.4240
fax 501.364.3155
visit us on the web at: www.archildrens.org
******************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************************
The information contained in this message may be privileged and confidential
and protected from disclosure. If the reader of this message is not the
intended recipient, or an employee or agent responsible for delivering this
message to the intended recipient, you are hereby notified that any
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Thank you.
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Message: 12
Date: Fri, 4 Jun 2010 10:03:49 -0500
From: "McCormick, James" <JMcCormick <@t> schosp.org>
Subject: [Histonet] Unsubscribe.
To: "Histonet <@t> lists.utsouthwestern.edu"
<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<0CA58D7DD405854899D129A03276B133443D48970E <@t> EXCHCCRMB.schosp.org>
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Please unsubscribe my email from your list.
Thank you,
James B. McCormick, M.D.
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Message: 13
Date: Fri, 4 Jun 2010 11:30:43 -0400
From: "Santiago, Albert" <Albert.Santiago <@t> uphs.upenn.edu>
Subject: [Histonet] H&E Stainer Validations
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BB0F4D2041EED9458AD95A538DBC4CAE075D112F <@t> uphsmbx6.UPHS.UPENN.EDU>
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Hello fellow Histonetters, I'm going to Demo a new H&E stainer and I was
wondering if anyone knows if there's a standard H&E Validation protocol
or if it just varies from lab to lab? Thanks for your help.... :-)
Albert Santiago, HT(ASCP)
Laboratory Manager
Dermatopathology
215-662-6759/6539-office
215-520-3797-BB
215-662-7520-fax
We will be relocating, as of 6/14/2010
our lab will be located
at 3020 Market St. Ste. 201
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Message: 14
Date: Fri, 4 Jun 2010 11:54:52 -0400
From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] Unsubscribe.
To: "McCormick, James" <JMcCormick <@t> schosp.org>,
histonet <@t> lists.utsouthwestern.edu
Message-ID:
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No.
Towns are like people. Old ones often have character, the new ones are
interchangeable.
--Wallace Stegner, Angle of Repose
On Fri, Jun 4, 2010 at 11:03 AM, McCormick, James <JMcCormick <@t> schosp.org>wrote:
> Please unsubscribe my email from your list.
>
> Thank you,
> James B. McCormick, M.D.
>
> ________________________________
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