[Histonet] Vibrotoming otoliths

[Charles.Scouten <@t> leica-microsystems.com]

Carve a channel in balsa wood, available at hobby shops, and sandwich
the otolith in.  Section wood and all.
 
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
 
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Telephone 630 964 0501
facsimile +1 630 964 0576
www.MyNeuroLab.com <http://www.myneurolab.com/> 
www.leica-microsystems.com <http://www.leica-microsystems.com/> 
 
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From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of jason
christian <jchris36 <@t> gmail.com>
Sent: Wednesday, May 26, 2010 2:27 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Vibrotoming otoliths
 
I am trying to section an otolith from a small minnow species with a 
vibrotome. The bone seems to be very dense and doesn't hold very well
inside 
any mounting media that I have tried. Does anyone have any suggestions
as to 
a media that will section smoothly but would be dense enough to hold the
small bone in the media while its being sectioned. Thank you in advance.
Jason 
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