[Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question

Liz Chlipala liz <@t> premierlab.com
Fri Jul 30 13:03:12 CDT 2010


We have not used that antibody but we have used the following antibody
from Serotec with good success.

Protocol:  Mouse Neutrophil Immunohistochemical Stain
Clone: 7/4
Vendor: Serotec
Catalog Number: MCA771
Species: Mouse
Isotype: Rat IgG2a


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949 
fax (303) 682-9060
Ship to Address:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Connolly, Brett M
Sent: Friday, July 30, 2010 11:34 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] FW: anti-Ly-6G (aka GR-1 ) IHC question 

Resending - 

> Does anyone have experience with any anti-mouse LY-6G antibodies on
> FFPE mouse tissue for staining neutrophils??
> Looking for antibodies and protocol suggestions.
> Thanks,
> Brett
> Brett M. Connolly, Ph.D.
> Molecular Imaging Team Leader
> Merck & Co., Inc.
> PO Box 4, WP-44K
> West Point, PA 19486
> brett_connolly <@t> merck.com
> T- 215-652-2501
> F- 215-993-6803
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