[Histonet] RES: Histonet Digest, Vol 80, Issue 32

Wed Jul 28 05:28:01 CDT 2010

Vanusa,

Não tenho permissão para mexer no Igecor e não entendi o que deveria ser
arrumado.

Att,

Thalita Ferraz
Produção
+55 12 3203-0612 (DIRETO)
+55 12 3203-0633 (SAC)
www.cipax.com.br
thalita <@t> cipax.com.br

-----Mensagem original-----
De: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Em nome de
histonet-request <@t> lists.utsouthwestern.edu
Enviada em: terça-feira, 27 de julho de 2010 14:07
Para: histonet <@t> lists.utsouthwestern.edu
Assunto: Histonet Digest, Vol 80, Issue 32

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Today's Topics:

   1. Re:  [Sectioning]  Brain Tissue (Nancy Herman)
   2. Re: Powdered reagent expiration dates (Pat Laurie)
   3. Tissue processing protocol for Hamster tissues (abijag )

----------------------------------------------------------------------

Message: 1
Date: Tue, 27 Jul 2010 11:25:54 -0400
From: "Nancy Herman" <Nancy.Herman <@t> inspection.gc.ca>
Subject: [Histonet] Re:  [Sectioning]  Brain Tissue
To: <histonet <@t> lists.utsouthwestern.edu>,	"Margaret Sherwood"
	<MSHERWOOD <@t> PARTNERS.ORG>
Message-ID: <4C4EA622.6DEB.00DD.0 <@t> inspection.gc.ca>
Content-Type: text/plain; charset=US-ASCII

We keep our water bath at 40-41 C.  Sometimes we add some 70% ethanol to the
water bath and this helps to get the wrinkles out (especially for cerebellum
and mice brain).  Most of our tissue is bovine brain but we deal with
multiple other species including mice.  We use McCormick Paraplast Plus
embedding Medium from Fisher.

Nancy Herman
Canadian Food Inspection Agency - Lethbridge Laboratory

>>> "Sherwood, Margaret " <MSHERWOOD <@t> PARTNERS.ORG> 2010/07/27 9:14 am >>>
To All:

We have been experiencing problems sectioning mouse brain.  The sectioning
is
fine, but we have problems in the water bath.  At 45 degrees C (peel-a-way
paraffin-Polysciences), the sections break apart but we don't get wrinkles.
With paraplast extra, we use 36 degrees C, but the sections are wrinkled and
disintegrate.   

I would appreciate hearing from listers as to what you do:  what temperature
is
your water bath, what embedding media do you use, etc?  Any help would be
appreciated!

Thanks!
Peggy

Peggy Sherwood
Lab Associate, Photopathology 
Wellman Center for Photomedicine (EDW 214)
Massachusetts General Hospital
55 Fruit Street
Boston, Massachusetts 02114-2696
617-724-4839 (voice mail)
617-726-6983 (lab)
617-726-1206 (fax)
msherwood <@t> partners.org 

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------------------------------

Message: 2
Date: Tue, 27 Jul 2010 09:00:20 -0700
From: Pat Laurie <foreightl <@t> gmail.com>
Subject: Re: [Histonet] Powdered reagent expiration dates
To: lpwenk <@t> sbcglobal.net
Cc: Histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<AANLkTi=wpvCnpxM50N0yOzKrSzEL4e8Y-U=NrCf4s5iA <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Everyone,

Thanks for your help, based off your explanations, statements and citations,
we will attempt to protest this and have it expunged.

On Mon, Jul 26, 2010 at 4:41 PM, Lee & Peggy Wenk
<lpwenk <@t> sbcglobal.net>wrote:

> See if you can get the following article. Biotech Histochem is published
by
> the Biological Stain Commission. http://www.biologicalstaincommission.org/
>
> Biotech Histochem. 2009 Feb;84(1):11-5.
>
> Stain and dye stability over a 30-year period: a comparison of certified
> dye
> powders by the Biological Stain Commission.
> Penney DP, Frank M, Fagan C, Willis C.
>
> Biological Stain Commission, Department of Pathology & Laboratory
Medicine,
> University of Rochester Medical Center, Rochester, New York 14642-0001,
> USA.
> David_Penney <@t> urmc.rochester.edu
>
> Abstract
> The Biological Stain Commission (BSC) Assay Laboratory has received
> numerous
> inquiries during the past several years regarding the long-term stability
> of
> stain and dye powders, particularly since packaging requirements call for
> expiration dates on reagents. We have conducted a study to examine the
> long-term stability of selected dye powders. We used the standard
> procedures
> of the BSC for testing biological stains for certification to give an
> indication of the long-term chemical stability as well as staining
> performance of the dye powders. An earlier study by Emmel and Stotz
> examined
> the stability of various dye powders after a five-year storage period. The
> present study is a follow-up project covering the same dyes after storage
> for 30 years. The dye samples chosen for the study are the same samples
> used
> in the five-year storage period study and give comparative results for all
> three time periods. The results of this study affirm the generally held
> speculation that dye powders are stable for many years and thus have a
> substantial shelf-life.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> Beaumont Hospital
> Royal Oak, MI 48073
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pat Laurie
> Sent: Monday, July 26, 2010 1:27 PM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Powdered reagent expiration dates
>
>  We were inspected by CAP on friday and we were cited for
>
>
> ANP.21366  *Are reagents and solutions properly labeled, as applicable and
> appropriate, with the following elements?*
>
> * *
>
> 1.      *Content and quantity, concentration or titer*
>
> 2.      *Storage requirements*
>
> 3.      *Date prepared or reconstituted by laboratory*
>
> 4.      *Expiration date*
>
> Specifically that our staining powders didn't have an expiration date
> printed on the bottle. All of our reconsituted reagents which are in use
> were dated with an expiration date properly though. I have always assumed,
> perhaphs incorrectly, that powdered stains never expire.  We have powders
> like Luxol Echt Blau, etc. that were purchased and opened over 40
> years ago.    If so, then these powdered reagents have gone through CAP
> inspections since the beginning and this inspector was the first one to
> find
> this problem.  Is this one that we might protest?
>
> --
> Patrick Laurie HT(ASCP)QIHC
> CellNetix Pathology & Laboratories
> 1124 Columbia Street, Suite 200
> Seattle, WA 98104
> PH: 206-215-5949
> plaurie <@t> cellnetix.com
>  _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>

-- 
Patrick Laurie HT(ASCP)QIHC
CellNetix Pathology & Laboratories
1124 Columbia Street, Suite 200
Seattle, WA 98104
PH: 206-215-5949
plaurie <@t> cellnetix.com

------------------------------

Message: 3
Date: 27 Jul 2010 15:30:26 -0000
From: "abijag " <abijag76 <@t> rediffmail.com>
Subject: [Histonet] Tissue processing protocol for Hamster tissues
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <20100727153026.31966.qmail <@t> f6mail-144-201.rediffmail.com>
Content-Type: text/plain; charset="UTF-8"

Dear Histonetters,

We were asked to process Hamster tissue(Large intestine) for one of our
investigators. I would like to know whether any of our users process hamster
tissues in their labs, if so kindly provide me a protocol for the same. Our
tissue processor is Sakura VIP6.

Thanks for all

Abi jagannath

------------------------------

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End of Histonet Digest, Vol 80, Issue 32
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