[Histonet] question for a friend

[Andrea Grantham]

A friend is asking this question and we were looking for responses  
telling what others would do in this situation:


  I am hoping to submit some brains for paraffin-embedded sectioning
soon, but we started intending to go the frozen-section route, so I
have some questions as to the feasibility of paraffin-embedding with
our storage conditions.  We began by fixing our brain-tissue with
paraformaldehyde (transcardial-perfusion).  Then, we stored in
paraformaldehyde for 4 hours before switching to 10% sucrose
overnight.  The next day we switched to 30% sucrose and stored
overnight (both sucrose storage at 4 degrees Celsius).  The brains
were then switched over to a cryoprotectant (which contains PBS,
sucrose, polyvinylpyrrolidone, and ethylene glycol) and stored at -20
degrees Celsius.  The brains have been in the cryoprotectant for
about a week, now.  Do you see anything in these storage conditions
that would prevent you from being able to paraffin-embed and section
these brains?  Thank you much.



Thanks,
Andi Grantham





Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cell Biology and Anatomy
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724

algranth <@t> email.arizona.edu
Tel: 520.626.4415     Fax: 520.626.2097

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