[Histonet] Brain Problems
Kimberly.Poole <@t> drdc-rddc.gc.ca
Tue Jul 27 09:27:58 CDT 2010
I am currently using rat brain for histology but something is not working. I follow the protocol below for tissue processing.
27 hours 20 minutes
I embedded my brains and then cut them afterwards. When the brain section hits the water, it looks like a blob of fat on the water. Then it spreads reall, really slowly. If a cut is made to the paraffin the brain bursts open and it falls apart. Am I doing something wrong in the processing stage? Is the tissue dehydrated too much. Any feedback would be greatly appreciated.
Kimberly Poole B.Sc
Casualty Management Section | Section de la gestion des blessés
Defence Research and Development Canada Suffield | Recherche et développement pour la défense Canada Suffield
Medicine Hat, AB, Canada T1A 8K6
kimberly.poole <@t> drdc-rddc.gc.ca <mailto:kimberly.poole <@t> drdc-rddc.gc.ca>
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