[Histonet] RE: Re water problem

Goins, Tresa TGoins <@t> mt.gov
Mon Jul 26 10:39:05 CDT 2010


Thanks for the great tip Steve - it works great!


Tresa Goins
Veterinary Diagnostic Lab
Department of Livestock
Bozeman, Montana



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Steven Weston
Sent: Sunday, July 25, 2010 9:50 PM
To: histonet list
Subject: [Histonet] Re water problem

We had this problem when we started using APTS coated slides and left out adhesive from the water bath. A simple solution I have found is to add a single drop of triton x100 (or similar detergent) to my full water bath and mix before heating the water. This reduces the surface tension of the water and allows it to run off the slide.
Regards
Steve weston
Menzies Research Institute
Hobart Tasmania, Australia
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