[Histonet] Water collecting at bottom of sections

V. Neubert histonet.nospam <@t> vneubert.com
Fri Jul 23 14:01:07 CDT 2010

I experienced it almost daily and thought this was normal!?

I just dipped the slides gently on a paper towel right after getting 
them on the slide and shaking, for the case I could not just shake it 
off. Indeed, as I remember, the Super Frost Plus slides had to be dipped 
a bit harder to let the water rinse off.

> Hello all,
> > From time to time and depending on what brand of adhesive
> (or charged) slides I am using, I seem to get a "bag" of
> water that drains to the bottom of my sections but doesn't
> drain "out".
> I have been working in microtomy a long time and have had to
> deal with this contingency time and time again, but never
> really have gotten to the bottom of the problem. I spoke
> with a premium manufacturer of such slides and they seemed
> to indicate that it is a problem with the coating, but
> couldn't tell me for sure.
> All I know is that certain brands do this more than others.
> If you know what I mean, you know it is a problem. My bath
> is pure distilled H2O with no gelatin or Sta-on added. It is
> if the adhesive properties are SO good that they will not
> release the water when vertically drained and have to be
> shaken off or cut with a razor blade at bottom to release
> the water.
> Anyway, if anyone has an insight or two on this, I would be
> interested. It seem sthe most challenging issues are ones
> that seem related to some of the most simple tasks that one
> has performed for many years!! Manufacturers understand what
> I mean, but cannot pinpoint the problem for me via phone or
> e-mail.
> Anyone see this and have a chemical/mechanical solution they
> have developed over the years?
> Thanks!
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