[Histonet] human on human IHC

[andreahooper <@t> rocketmail.com]

Along those same lines I have conjugated primaries to biotin and it works beautifully for human on human IHC. Another alternative if extra amplification is needed is to conjugating to FITC and then using a rabbit-anti-FITC followed by an anti-rabbit polymer. 

Just be aware with human on human controls are imperative as you may get binding to human Fc receptors.

Andrea Hooper


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-----Original Message-----
From: <sgoebel <@t> xbiotech.com>
Sender: histonet-bounces <@t> lists.utsouthwestern.edu
Date: Thu, 15 Jul 2010 09:27:19 
To: Kim Merriam<kmerriam2003 <@t> yahoo.com>
Cc: Histonet<histonet <@t> lists.utsouthwestern.edu>
Subject: RE: [Histonet] human on human IHC


   I  am doing alot with this right now.  The best thing I havefound to
   do   is  to  conjugate your primary antibody with HRP, then just use
   the  direct   method  (no secondary), and it turns out beautifully!!
   You  can  buy thekit to do this from Fisher.  I  can get the exact
   order number if you need it.  Good Luck!!

   
   Sarah Goebel, B.A., HT (ASCP)

   Histotechnician

   XBiotech USA Inc.

   8201 East Riverside Dr. Bldg 4 Suite 100
   Austin, Texas  78744
   (512)386-5107

   -------- Original Message --------
   Subject: [Histonet] human on human IHC
   From: Kim Merriam <[1]kmerriam2003 <@t> yahoo.com>
   Date: Thu, July 15, 2010 8:00 am
   To: Histonet <[2]histonet <@t> lists.utsouthwestern.edu>
   Hi,
   Does   anyone   know  of  a  human-on-human  IHC  kit  similar to  the
   mouse-on-mouse kits
   that are available?
   Thanks,
   Kim
    Kim Merriam, MA, HT(ASCP)QIHC
   Cambridge, MA
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References

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