[Histonet] Re: Histonet Digest, Vol 80, Issue 14
AWeiss <@t> shorememorial.org
AWeiss <@t> shorememorial.org
Tue Jul 13 14:02:09 CDT 2010
I think Sue is upset about sending the e-mail to more than one person. I
sent it to u and to her, do u know what she is talking about?
Andrea J Weiss BST CT (ASCP)
Cytotechnologist
609 653 3577 Ext 4907
aweiss <@t> shorememorial.org
histonet-request@
lists.utsouthwest
ern.edu To
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Histonet Digest, Vol 80, Issue 14
07/13/2010 01:02
PM
Please respond to
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Today's Topics:
1. Difference between Protease and Proteinase-K? (Jennifer Campbell)
2. Phospho-Histone 3 (SER10) antibody (Martha Ward)
3. Re: Difference between Protease and Proteinase-K? (Kathleen Jones)
4. RE: Phospho-Histone 3 (SER10) antibody (Connolly, Brett M)
5. Great Histotech Job in South Carolina (Alisha Dynan)
6. RE: air dry vs heat dry heat (Jeffrey Thompson)
7. RE: air dry or heat dry (Mejia, Maria)
8. BOND III VS ULTRA BENCH MARK (Ade Tunde)
9. anti mouse CD163 (Mauger, Joanne)
10. CORRECT BILLING? (Sara Baldwin/mhhcc.org)
11. Histologist opportunity for Private Dermatology practice in
Phoenix AZ (jcox90 <@t> yahoo.com)
12. Re: CORRECT BILLING? (DKBoyd <@t> chs.net)
13. off-topic "cryosectioning" as security check word (Kim Merriam)
----------------------------------------------------------------------
Message: 1
Date: Mon, 12 Jul 2010 10:07:30 -0700
From: "Jennifer Campbell" <jcampbell <@t> vdxpathology.com>
Subject: [Histonet] Difference between Protease and Proteinase-K?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<5658CBDB9EAE6545ABE50D2563D81BF83CFFD2 <@t> VDXSERVER01.vdxpathology.local>
Content-Type: text/plain; charset="us-ascii"
Hi All,
I would like to try enzyme digestion on my CD31 (for K9 and feline
tissue) which I haven't been able to get to work using citrate buffer pH
6.0 or pH 9.0. I have heard recommendations for both Protease and
Proteinase-K but, do not know which one I should try out. Any feedback
on the differences between the two?
Thanks,
Jennifer Campbell
------------------------------
Message: 2
Date: Mon, 12 Jul 2010 13:10:27 -0400
From: "Martha Ward" <mward <@t> wfubmc.edu>
Subject: [Histonet] Phospho-Histone 3 (SER10) antibody
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<61135F0455D33347B5AAE209B903A30433DEBB14 <@t> EXCHVS2.medctr.ad.wfubmc.edu>
Content-Type: text/plain; charset="us-ascii"
I have been trying without success to get this antibody to work. Is
anyone doing this antibody? We have the Leica Bond Max and would like
to perform it on this platform. I have tried 2 antibodies from Cell
Signaling Technology without any success. Any advice would be greatly
appreciated.
Thanks!
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104
------------------------------
Message: 3
Date: Mon, 12 Jul 2010 14:35:40 -0300
From: "Kathleen Jones" <Kjones <@t> upei.ca>
Subject: Re: [Histonet] Difference between Protease and Proteinase-K?
To: <histonet <@t> lists.utsouthwestern.edu>, "Jennifer Campbell"
<jcampbell <@t> vdxpathology.com>
Message-ID: <4C3B283D0200008B00027834 <@t> grpwise.novell.upei.ca>
Content-Type: text/plain; charset=US-ASCII
Hello Jennifer
I'm not sure what platform you are using, but from my experience, I
obtain beautiful results for CD31 in canine and feline tissue, but the
protocols for each are quite different. For CD31 in canine tissue I use
a tris based buffer and for feline I use protease.
I know this doesn't answer you question, but thought I would pass along
what works for me.
Good Luck
Kathy
Kathleen Jones
Research Technician
Pathology/Microbiology
AVC - UPEI
(902)566-0595
>>> "Jennifer Campbell" <jcampbell <@t> vdxpathology.com> 7/12/2010 2:07 PM
>>>
Hi All,
I would like to try enzyme digestion on my CD31 (for K9 and feline
tissue) which I haven't been able to get to work using citrate buffer
pH
6.0 or pH 9.0. I have heard recommendations for both Protease and
Proteinase-K but, do not know which one I should try out. Any
feedback
on the differences between the two?
Thanks,
Jennifer Campbell
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 4
Date: Mon, 12 Jul 2010 13:58:43 -0400
From: "Connolly, Brett M" <brett_connolly <@t> merck.com>
Subject: RE: [Histonet] Phospho-Histone 3 (SER10) antibody
To: "Martha Ward" <mward <@t> wfubmc.edu>,
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<63EA0607835FBA4689CEA9EA8B482692033ED4D9 <@t> usctmx1141.merck.com>
Content-Type: text/plain; charset="us-ascii"
Martha,
We have good success with Millipore 06-570 using citrate HIER - also
with the (pSer28) Histone H3 from Epitomics (#1329).
Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly <@t> merck.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha
Ward
Sent: Monday, July 12, 2010 1:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Phospho-Histone 3 (SER10) antibody
I have been trying without success to get this antibody to work. Is
anyone doing this antibody? We have the Leica Bond Max and would like
to perform it on this platform. I have tried 2 antibodies from Cell
Signaling Technology without any success. Any advice would be greatly
appreciated.
Thanks!
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
336-716-2104
_______________________________________________
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------------------------------
Message: 5
Date: 12 Jul 2010 15:08:52 -0400
From: Alisha Dynan <alisha <@t> ka-recruiting.com>
Subject: [Histonet] Great Histotech Job in South Carolina
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <468896864.1278961732674.JavaMail.cfservice <@t> SL4APP1>
Content-Type: text/plain; charset="utf-8"
Dear Histonet Members,
I hope you are doing well. I am a recruiter at a highly successful and well
respected Healthcare recruiting firm. I help place Lab Professionals in
permanent positions across the country and I wanted to see if you are
interested in exploring other career opportunities? We are completely free
of charge to candidates and and we work on quite a few histotechnician,
histotechnologist, and cytotechnologist openings across the country. Our
clients typically assist with relocation expenses.
One particular client I am working with is looking for a Histotech for a
hospital lab in South Carolina. They are looking for someone with 1+ year
experience, an HT(ASCP) or HTL(ASCP) certification and someone who is
familiar with histology, IHC, and frozen sections.
Below is a list of some of the opportunities we are currently working on.
If you do not see an opening in a location in which you live or would like
to live, please send me an email me a copy of your resume and let me know
where you would be interested in a job. I will then tailor a search for you
that is completely confidential and free to candidates.
Current HT and HTL Opportunities:
1. Histotech - Indianapolis, IN
2. Histotechnologist - Atlanta, GA
3. Histotech - NYC
4. Histology Supervisor - Las Vegas, NV
5. Grossing Tech - NYC
6. Histotech - MA
7. IHC Technologist - MA
8. Histology Supervisor - GA
9. Histotech - Otsego County, NY
If you're interested in learning more about these opportunities or
opportunities in a certain geographic location please reply with an updated
resume and let me know when a good time to reach you is.
If this is not the right fit for you please let me know who you can
recommend and give me an idea of what types of positions you'd be
interested in hearing about in the future. I cover the entire US and have
am working on Lab positions at all levels. We offer a very generous
referral bonus for anyone you refer to us that we place into any position
across the country.
To view some additional opportunities please visit our website at
www.ka-recruiting.com.
Sincerely,
Alisha (Taylor) Dynan, Founder
K.A. Recruiting, Inc.
Your Partner in Healthcare Recruiting
10 Post Office Square 8th Floor SOUTH
Boston, MA 02109
P: (617) 692-2949
F: (617) 507-8009
alisha <@t> ka-recruiting.com
www.ka-recruiting.com
------------------------------
Message: 6
Date: Mon, 12 Jul 2010 15:10:55 -0600
From: "Jeffrey Thompson" <JefThompson <@t> salud.unm.edu>
Subject: [Histonet] RE: air dry vs heat dry heat
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4C3B307F0200004D000C9F06 <@t> hsc-iagate1.health.unm.edu>
Content-Type: text/plain; charset=US-ASCII
Hello,
Thanks for all the replies. I find them helpful and it may help to resolve
some of our lab 'discussions'.
Thanks again,
Jeff
------------------------------
Message: 7
Date: Mon, 12 Jul 2010 15:55:33 -0700
From: "Mejia, Maria" <Maria.Mejia <@t> ucsf.edu>
Subject: RE: [Histonet] air dry or heat dry
To: Jeffrey Thompson <JefThompson <@t> salud.unm.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BD7845710F83B94796F4534A6D6EC71C14B2D80008 <@t> EX02.net.ucsf.edu>
Content-Type: text/plain; charset="us-ascii"
Hello Jeff,
Let's see you are sectioning your (fixed) rat brain on the cryostat at
10ums. I'm assuming they are mounted on standard size superfrost
plus slides & since they are fixed sections & even if they were not fixed -
after sectioning why not air-dry these sections standing up under
a fan for 2 hours or longer and store them in plastic slide boxes that hold
either 5, 10, 15, or more slides. Only store enough slides
in each box for a particular IHC run. Since I don't know what you are
staining for - I don't know if storing fixed cryostat sections at -80C is
necessary. This is your protocol.
Seal each plastic box with cryo tape & place inside a zip-loc bag - fold
the bags to the size of box & hold in place with rubber band.
Place boxes inside a labeled 3-inch cryo box & store at -80C. When you are
ready to run IHC, just remove only what you need
from the -80C freezer & quickly return the rest back inside -80C. Even
though these sections are fixed (& for those not fixed), once your
remove slide boxes from -80C freezer - I would not open the box. Let them
equilibriate at room temp on bench top for about 30 mins before
removing from plastic bag & slide box. Then run your IHC protocol.
Regards
Maria Mejia
Histology Supervisor
Department of Neurosurgery
UCSF
San Francisco, CA 94103
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu
[histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffrey Thompson
[JefThompson <@t> salud.unm.edu]
Sent: Friday, July 09, 2010 2:21 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] air dry or heat dry
Hello,
We have an ongoing debate in our lab regarding the relative virtue of heat
drying slides vs RT air drying them.
Tissues: 4% paraformaldehyde perfused rat brains frozen in OCT blocks
Sections: 10 microns on a cryostat (at approx -25 C)
Slides: Superfrost Plus
The slides are stored in slide boxes at -80 C until staining.
When staining the heat dry faction (wanting to avoid icy slides) put their
slide boxes straight out of the freezeer into a 50 C oven for 30 minutes
before taking out slides to stain. then the box goes back to the freezer
until the next round of staining,
The air dry group feels that cooking the antigens repeatedly at 50 C is
problematic so they take the frosty slides out their slide boxes and return
them ASAP to the freezer. The slides to be stained are air dried in the
fume hood for about 30 min.
A third, middle of the road, person takes her slides out of the cold boxes
and then puts the slides in a 60 C oven for 30 minutes.
For all three groups then, the slides are given a PAP pen border to prepare
for IHC and when the pen solution is dry, 10 minutes in acetone and the
remainder of the staining procedure.
So my question is: Who is using the best technique?
Another hotly debated topic is wether it is advisable to put a drop of PBS
on the slide before 'sticking' the section to prevent folds in the
sections..
Any opinions are appreciated.
Thanks,
Jeff
_______________________________________________
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Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 8
Date: Mon, 12 Jul 2010 16:51:03 -0700 (PDT)
From: Ade Tunde <atunde90 <@t> yahoo.com>
Subject: [Histonet] BOND III VS ULTRA BENCH MARK
To: histonet <@t> lists.utsouthwestern.edu
Message-ID: <61269.48112.qm <@t> web46105.mail.sp1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
I need help about these immunostainers, Does anybody has idea about the
best out
of the two?
I want to know the ADVANTAGES AND DISADVANTAGES of each.Thanks GUY
Tunde Ajibade BS HTL(ASCP)QIHC
Pathology lab
Newark Beth Israel Medical Center.
Newark NJ 07111
------------------------------
Message: 9
Date: Tue, 13 Jul 2010 08:45:01 -0400
From: "Mauger, Joanne" <MAUGER <@t> email.chop.edu>
Subject: [Histonet] anti mouse CD163
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<443F5B475A9BF647AB962E834884EBAD278D12780B <@t> EX7CCRPW03V1.chop.edu>
Content-Type: text/plain; charset="us-ascii"
Hi All,
Can anyone reccomend a good CD163 antibody that works in mouse FFPE
tissue?
Thanks,
Jo
Joanne Mauger HT(ASCP)QIHC
Children's Hospital of Philadelphia
________________________________________
------------------------------
Message: 10
Date: Tue, 13 Jul 2010 09:35:05 -0400
From: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
Subject: [Histonet] CORRECT BILLING?
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<OFFBDF0999.1A8EF30D-ON8525775F.004A9FB0-8525775F.004A9FB5 <@t> LocalDomain>
Content-Type: text/plain; charset=ISO-8859-1
HISTONETTERS
Another question for CPT codes on NON-GYNS (Pelvic washing and etc) and a
cell block
do you use 88160 & 88305?
Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin <@t> mhhcc.org
Ph 812-482-0210, 482-0216, Fax 812-482-0232,
Pager 812-481-0897
Confidential information, Authorized use only.
------------------------------
Message: 11
Date: Tue, 13 Jul 2010 07:07:11 -0700 (PDT)
From: jcox90 <@t> yahoo.com
Subject: [Histonet] Histologist opportunity for Private Dermatology
practice in Phoenix AZ
To: "Histonet <@t> Lists. Utsouthwestern. Edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <202375.45513.qm <@t> web56804.mail.re3.yahoo.com>
Content-Type: text/plain; charset=us-ascii
We have an immediate opening for a histologist in our Dermatology practice.
Full
time, excellent pay and benefits, great Doctors, flexible hours. Must be
able to
work independently with little supervision. Please call Jill at
602-481-1424 for
more details or email resume to jcazderm <@t> yahoo.com.
Jill Cox HT (ASCP)
------------------------------
Message: 12
Date: Tue, 13 Jul 2010 10:12:54 -0400
From: DKBoyd <@t> chs.net
Subject: Re: [Histonet] CORRECT BILLING?
To: "Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
Cc: histonet <@t> lists.utsouthwestern.edu,
histonet-bounces <@t> lists.utsouthwestern.edu
Message-ID:
<OFED9340B0.BB8FCBC0-ON8525775F.004DFB91-8525775F.004E15F9 <@t> chs.net>
Content-Type: text/plain; charset="US-ASCII"
We prepare cytospins and charge 88108 for the cytospin preparation and
88305 for the cell block.
Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical
Center I
200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F:
804-765-5582 l dkboyd <@t> chs.net
"Sara Baldwin/mhhcc.org" <sbaldwin <@t> mhhcc.org>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
07/13/2010 09:38 AM
To
<histonet <@t> lists.utsouthwestern.edu>
cc
Subject
[Histonet] CORRECT BILLING?
HISTONETTERS
Another question for CPT codes on NON-GYNS (Pelvic washing and etc) and a
cell block
do you use 88160 & 88305?
Thanks
Pathology Supervisor
Kathy Baldwin, SCT (ASCP)
Memorial Hospital and Health Care Center
sbaldwin <@t> mhhcc.org
Ph 812-482-0210, 482-0216, Fax 812-482-0232,
Pager 812-481-0897
Confidential information, Authorized use only.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 13
Date: Tue, 13 Jul 2010 08:40:04 -0700 (PDT)
From: Kim Merriam <kmerriam2003 <@t> yahoo.com>
Subject: [Histonet] off-topic "cryosectioning" as security check word
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <751686.44717.qm <@t> web50305.mail.re2.yahoo.com>
Content-Type: text/plain; charset="iso-8859-1"
Hi All,
I thought you all would get a kick out of this. I was ordering some
tickets on
ticketmaster and look at the word that popped up for the security check -
it was
"cryosectioning"!
I managed to get a screenshot of it; it is attached. We have infiltrated
main-stream culture!
Kim
Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
------------------------------
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