[Histonet] Phospho-Histone 3 (SER10) antibody

Connolly, Brett M brett_connolly <@t> merck.com
Mon Jul 12 12:58:43 CDT 2010

We have good success with Millipore 06-570 using citrate HIER - also
with the (pSer28) Histone H3 from Epitomics (#1329).

Brett M. Connolly, Ph.D.
Molecular Imaging Team Leader
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
tel. 215-652-2501 fax. 215-993-6803
brett_connolly <@t> merck.com

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Martha
Sent: Monday, July 12, 2010 1:10 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Phospho-Histone 3 (SER10) antibody

I have been trying without success to get this antibody to work.   Is
anyone doing this antibody?  We have the Leica Bond Max and would like
to perform it on this platform.  I have tried 2 antibodies from Cell
Signaling Technology without any success.   Any advice would be greatly
Martha Ward, MT (ASCP) QIHC
Assistant Manager, Molecular Diagnostics Lab
Wake Forest University Baptist Medical Center
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